Description

This master thesis describes protocols for extracellular vesicles (EV)-depleted cell culture, infection experiments with C. pneumoniae and subsequent EV isolation via differential ultracentrifugation as well sample quality control by bicinchoninic acid (BCA) assay and Western blot. Infectious progeny assay and ompA PCR were used to determine the grade of infection.
PeriodAug 2023Jun 2024