Projects per year
Organization profile
Our work aims to understand the structure, assembly and function of biological macromolecule complexes. We embed unstained, unfixed specimens in vitreous ice to preserve their structures. Transmission cryo-electron microscopy is then used to visualize the specimens, but the images recorded are difficult to interpret because they are projections of the specimen degraded by noise. Thus we computationally combine images of the specimen viewed from different angles enabling us to reconstruct a three-dimensional model. Any inherent symmetry present in the structure is also used in this averaging. This method is particularly useful for objects that are too large, unstable or variable to be studied by X-ray crystallography or NMR, such as enveloped viruses.
Fields of Science
- 118 Biological sciences
- virology, structural biology, cryo-electron microscopy
International and National Collaboration
Profiles
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Maria Anastasina
- Molecular and Integrative Biosciences Research Programme - University Researcher
- Institute of Biotechnology
- Macromolecular structure and function
- Doctoral Programme in Microbiology and Biotechnology - Supervisor for doctoral programme
Person: U3 Research and teaching staff, UH
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Sarah Butcher
- Molecular and Integrative Biosciences Research Programme - Professor
- Institute of Biotechnology
- Macromolecular structure and function
- Doctoral Programme in Integrative Life Science - Supervisor for doctoral programme
- Doctoral Programme in Microbiology and Biotechnology - Supervisor for doctoral programme
Person: UH , U4 Research and teaching staff
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Ausra Domanska
- Molecular and Integrative Biosciences Research Programme - University Researcher
- Macromolecular structure and function
Person: U3 Research and teaching staff, UH
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Sergei Gurianov
- Molecular and Integrative Biosciences Research Programme - Postdoctoral Researcher
- Macromolecular structure and function
Person: U2 Teaching and research staff, UH
Equipment
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Instruct-ERIC Centre Finland
Sarah Butcher (Manager), Hanna M Oksanen (Other), Minna Poranen (Other), Katri Eskelin (Other), Perttu Permi (Other), Markku Varjosalo (Other), Lari Lehtiö (Other) & Juha Rouvinen (Other)
Faculty of Biological and Environmental SciencesFacility/equipment: Core Facility
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Instruct-HiLIFE CryoEM core facility
Sarah Butcher (Manager), Ausra Domanska (Operator), Pasi Laurinmäki (Operator) & Behnam Lak (Operator)
Institute of BiotechnologyFacility/equipment: Core Facility
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Structural Biology Finland (FINStruct)
Sarah Butcher (Manager), Hanna M Oksanen (Other), Minna Poranen (Other), Katri Eskelin (Other), Tommi Kajander (Other), Perttu Permi (Other), Lari Lehtiö (Other), Juha Rouvinen (Other), Tiina A Salminen (Other) & Anastassios Papageorgiou (Other)
Faculty of Biological and Environmental SciencesFacility/equipment: Core Facility
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Integrated Structural Biology Infrastructure-European Research Infrastructure Consortium (Instruct-ERIC) / Consortium FINStruct and Instruct-ERIC
Butcher, S., Poranen, M., Varjosalo, M., Kajander, T., Oksanen, H. M., Eskelin , K., Lehtiö, L., Salminen, T. A. & Papageorgiou, A. C.
01/01/2022 → 31/12/2026
Project: Academy of Finland: Research infrastructure
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Structural Biology Finland (FINStruct) & Instruct-ERIC Centre FI / Research Infrastructure Roadmap 2021-2024
Butcher, S., Poranen, M., Kajander, T., Iwai, H., Varjosalo, M., Lehtiö, L., Rouvinen, J., Salminen, T. A., Papageorgiou, A. C., Oksanen, H. M. & Eskelin , K.
01/01/2021 → 31/12/2024
Project: Academy of Finland: Research infrastructure
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Instruct-HiLIFE: Integrated structural cell biology research infrastructure platform, HiLIFE, University of Helsinki
Poranen, M., Butcher, S., Iwai, H., Kajander, T., Oksanen, H. M. & Eskelin , K.
01/01/2021 → 31/12/2024
Project: Other project
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Integrated Structural Biology Infrastructure / Consortium: Instruct-Suomi
Butcher, S., Kajander, T., Lehtiö, L., Määttä, J. A. E., Papageorgiou, A., Rouvinen, J., Salminen, T., Iwai, H., Poranen, M., Eskelin , K. & Oksanen, H. M.
01/01/2020 → 31/12/2022
Project: Other project
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Genetic susceptibility for severe influenza infections
01/01/2012 → 31/12/2014
Project: Research project
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Simultaneous membrane and RNA binding by Tick-Borne Encephalitis Virus capsid protein
Pulkkinen, L. I. A., Barrass, S. V., Lindgren, M. K., Pace, H., Overby, A. K., Anastasina, M., Bally, M., Lundmark, R. M. & Butcher, S. J. M., Feb 2023, In: PLoS Pathogens. 19, 2, 24 p.Research output: Contribution to journal › Article › Scientific › peer-review
Open AccessFile -
Molecular Organisation of Tick-Borne Encephalitis Virus
Pulkkinen, L. I. A., Barrass, S., Domanska, A., Overby, A. K., Anastasina, M. & Butcher, S. J., Apr 2022, In: Viruses (Basel). 14, 4, 17 p., 792.Research output: Contribution to journal › Article › Scientific › peer-review
Open AccessFile -
SARS-CoV-2 Production, Purification Methods and UV Inactivation for Proteomics and Structural Studies
Plavec, Z., Domanska, A., Liu, X., Laine, P., Paulin, L., Varjosalo, M., Auvinen, P., Wolf, S. G., Anastasina, M. & Butcher, S. J., Sep 2022, In: Viruses-Basel. 14, 9, 12 p., 1989.Research output: Contribution to journal › Article › Scientific › peer-review
Open Access -
Structural Studies Reveal that Endosomal Cations Promote Formation of Infectious Coxsackievirus A9 A-Particles, Facilitating RNA and VP4 Release
Domanska, A., Plavec, Z., Ruokolainen, V., Löflund, B., Marjomaki, V. & Butcher, S., 21 Dec 2022, In: Journal of Virology. 96, 4, 20 p., e0136722.Research output: Contribution to journal › Article › Scientific › peer-review
Open AccessFile -
A comparative analysis of parechovirus protein structures with other picornaviruses
Domanska, A., Guryanov, S. & Butcher, S. J., 28 Jul 2021, In: Open biology. 11, 7, 14 p., 210008.Research output: Contribution to journal › Review Article › peer-review
Open AccessFile
Activities
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Instruct-ERIC course on macromolecular X-ray, electron, and neutron crystallography 2023
Sergei Gurianov (Attendee)
29 May 2023 → 2 Jun 2023Activity: Participating in or organising an event types › Organisation and participation in conferences, workshops, courses, seminars
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Pathogens (Journal)
Ausra Domanska (Reviewer)
18 Jan 2023Activity: Publication peer-review and editorial work types › Peer review of manuscripts
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ITQB NOVA, Lisbon University NOVA
Ausra Domanska (Visiting researcher)
8 Mar 2022 → 13 Mar 2022Activity: Visiting an external institution types › Academic visit to other institution
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Portuguese Centre for Integrated Structural Biology (PCISBIO) meeting
Ausra Domanska (Attendee) & Sarah Butcher (Speaker: Presenter)
11 Mar 2022Activity: Participating in or organising an event types › Organisation and participation in conferences, workshops, courses, seminars
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Catarina Paiva
Hanna M Oksanen (Host), Sarah Butcher (Host), Minna Poranen (Host) & Katri Eskelin (Host)
Aug 2022 → Nov 2022Activity: Hosting a visitor types › Academic visit at UH
Prizes
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FEMS Young Scientist Meeting Grant (YSMG)
Manole, Violeta (Recipient), 8 Sep 2009
Prize: Prizes and awards
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Helsingin Yliopiston HEBIOT-koulutusohjelma 800 euroa
Happonen, Lotta (Recipient), 14 Feb 2007
Prize: Prizes and awards
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Helsingin Yliopiston Juhlarahaston matka-apuraha 2500 euroa
Happonen, Lotta (Recipient), 26 Mar 2009
Prize: Prizes and awards
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Press/Media
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Hong Kong pro-democracy lawmakers to resign as Beijing moves to quash opposition
11/11/2020
1 item of Media coverage
Press/Media: Press / Media
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Structure of SARS-CoV-2 RNA-dependent RNA polymerase published
15/04/2020
1 item of Media coverage
Press/Media: Press / Media
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-UNIVERSITY OF HELSINKI: A new research infrastructure, Instruct Centre Finland, part of Instruct-ERIC, opens to scientists
Sarah Butcher & Hanna M Oksanen
24/02/2020
1 item of Media coverage
Press/Media: Press / Media
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A new research infrastructure, Instruct Centre Finland, part of Instruct-ERIC, opens to scientists
Sarah Butcher & Hanna M Oksanen
21/02/2020
1 item of Media coverage
Press/Media: Press / Media
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Scientists make major breakthrough that could pave the way for cure to common cold
18/12/2019
1 item of Media coverage
Press/Media: Press / Media