Organization profile

Organisation Profile

Our work aims to understand the structure, assembly and function of biological macromolecule complexes. We embed unstained, unfixed specimens in vitreous ice to preserve their structures. Transmission cryo-electron microscopy is then used to visualize the specimens, but the images recorded are difficult to interpret because they are projections of the specimen degraded by noise. Thus we computationally combine images of the specimen viewed from different angles enabling us to reconstruct a three-dimensional model. Any inherent symmetry present in the structure is also used in this averaging. This method is particularly useful for objects that are too large, unstable or variable to be studied by X-ray crystallography or NMR, such as enveloped viruses.

International and National Collaboration Publications and projects within past five years.

Equipment

Instruct-HiLIFE CryoEM core facility

Sarah Butcher (Manager), Ausra Domanska (Operator), Pasi Laurinmäki (Operator) & Benita Löflund (Operator)

Institute of Biotechnology

Facility/equipment: Core Facility

Projects 2012 2014

Genetic susceptibility for severe influenza infections

Anastasina, M.

01/01/201231/12/2014

Project: Research project

Publications 1997 2019

A 2.8-Angstrom-Resolution Cryo-Electron Microscopy Structure of Human Parechovirus 3 in Complex with Fab from a Neutralizing Antibody

Domanska, A., Flatt, J. W., Jukonen, J., Geraets, J. & Butcher, S. J., Feb 2019, In : Journal of Virology. 93, 4, 12 p., e01597-18.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
File

Adenovirus flow in host cell networks

Flatt, J. W. & Butcher, S. J., Feb 2019, In : Open biology. 9, 2, 11 p., 190012.

Research output: Contribution to journalReview ArticleScientificpeer-review

Open Access
File

A novel druggable interprotomer pocket in the capsid of rhino- and enteroviruses

Abdelnabi, R., Geraets, J. A., Ma, Y., Mirabelli, C., Flatt, J. W., Domanska, A., Delang, L., Jochmans, D., Kumar, T. A., Jayaprakash, V., Sinha, B. N., Leyssen, P., Butcher, S. J. & Neyts, J., Jun 2019, In : PLoS Biology. 17, 6, 17 p., 3000281.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
File

Antibacterial polymer fibres by rosin compounding and melt-spinning

Kanerva, M., Puolakka, A., Takala, T. M., Elert, A. M., Mylläri, V., Jönkkäri, I., Sarlin, E., Seitsonen, J., Ruokolainen, J., Saris, P. & Vuorinen, J., 8 May 2019, In : Materials Today Communications.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
File

Extracellular Albumin and Endosomal Ions Prime Enterovirus Particles for Uncoating That Can Be Prevented by Fatty Acid Saturation

Domanska, A., Butcher, S., Ruokolainen, V., Laajala, M., Palliccia, M. & Marjomäki, V., 13 Aug 2019, In : Journal of Virology. 93, 17, p. 1-19 19 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access

Activities 2000 2019

MicroBeta instrument demo

Sergei Gurianov (Attendee)
3 Jan 2019

Activity: Participating in or organising an event typesOrganisation and participation in conferences, workshops, courses, seminars

ScanVir2019 conference

Ausra Domanska (Speaker: Presenter)
3 Jun 20195 Jun 2019

Activity: Participating in or organising an event typesOrganisation and participation in conferences, workshops, courses, seminars

Workshop: How to stand out as a Presenter?

Maria Anastasina (Attendee)
17 Dec 2018

Activity: Participating in or organising an event typesOrganisation and participation in conferences, workshops, courses, seminars

13th Seili Virus-Cell Interaction Meeting

Ausra Domanska (Speaker: Presenter)
27 Aug 201829 Aug 2018

Activity: Participating in or organising an event typesOrganisation and participation in conferences, workshops, courses, seminars

MMB-401 Seminar in evolution of microbial pathogenesis 2018

Sergei Gurianov (Member of organizing committee)
30 Oct 201814 Dec 2018

Activity: Participating in or organising an event typesOrganisation and participation in conferences, workshops, courses, seminars

Prizes

FEMS Young Scientist Meeting Grant (YSMG)

Violeta Manole (Recipient), 8 Sep 2009

Prize: Prizes and awards

Helsingin Yliopiston HEBIOT-koulutusohjelma 800 euroa

Lotta Happonen (Recipient), 14 Feb 2007

Prize: Prizes and awards

Helsingin Yliopiston Juhlarahaston matka-apuraha 2500 euroa

Lotta Happonen (Recipient), 26 Mar 2009

Prize: Prizes and awards

Maikki Friberg award 2013

Sarah Butcher (Recipient), 5 Jun 2013

Prize: Prizes and awards