Research output per year
Research output per year
Viikinkaari 9, Biocenter 1, 6th floor, room 6215
00014 University of Helsinki
Finland
Research activity per year
Novel purification and analysis methods for macromolecular complexes.
Asymmetric flow field-flow fractionation (AF4) is a subtechnique of field-flow fractionation. It separates molecules according to differences in their diffusion coefficients, which in turn reflects their size and shape. AF4 is applicable to a broad range of molecular masses and sizes and molecules with sizes that range from 2 nm to 50 μm can be analyzed.
My research focuses on the development of optimized methods to purify large macromolecular complexes such as ribosomes (a complex of 80 ribosomal proteins and three ribosomal RNAs), viral assemblies as well as intact viruses of various sizes and morphologies. Furthermore, my research aims to use AF4 for detailed analysis of large macromolecular complexes. This can be achieved by analyzing complexes that have been treated with various chemicals, enzymes, temperatures etc.
I am combining the AF4 purification to other methods that include monolithic anion chromatography and centrifugation methods to enable large-scale purification of macromolecular complexes to detailed biochemical and structural analysis.
My previous research included studies on two different plant viruses (Cocksfoot mottle virus and Potato virus A) and applied projects on heterologous protein production in plants.
Research output: Contribution to journal › Article › Scientific › peer-review
Research output: Chapter in Book/Report/Conference proceeding › Chapter › Scientific › peer-review
Research output: Contribution to journal › Article › Scientific › peer-review
Research output: Contribution to journal › Article › Scientific › peer-review
Research output: Chapter in Book/Report/Conference proceeding › Entry for encyclopedia / dictionary › Scientific › peer-review
Butcher, S. (Principal Investigator), Poranen, M. (Co-Principal Investigator), Varjosalo, M. (Principal Investigator), Kajander, T. (Co-Principal Investigator), Oksanen, H. M. (Participant), Eskelin , K. (Participant), Lehtiö, L. (Principal Investigator), Salminen, T. A. (Participant) & Papageorgiou, A. C. (Participant)
01/01/2022 → 31/12/2026
Project: Research Council of Finland: Research infrastructure
Heinlein, M. (Project manager), Poranen, M. (Project manager), Kogel, K.-H. (Principal Investigator), Patel, A. (Principal Investigator), Eskelin , K. (Participant), Korhonen, S. M. (Participant), Levanova, A. (Participant), Westerholm, T. T. (Participant) & Zaytseva, A. (Participant)
01/06/2021 → 31/05/2025
Project: Ministry funding
Butcher, S. (Principal Investigator), Poranen, M. (Co-Principal Investigator), Kajander, T. (Co-Principal Investigator), Iwai, H. (Co-Principal Investigator), Varjosalo, M. (Co-Principal Investigator), Lehtiö, L. (Co-Principal Investigator), Rouvinen, J. (Co-Principal Investigator), Salminen, T. A. (Co-Principal Investigator), Papageorgiou, A. C. (Co-Principal Investigator), Oksanen, H. M. (Participant) & Eskelin , K. (Participant)
01/01/2021 → 31/12/2024
Project: Research Council of Finland: Research infrastructure
Poranen, M. (Project manager), Butcher, S. (Principal Investigator), Iwai, H. (Principal Investigator), Kajander, T. (Principal Investigator), Oksanen, H. M. (Participant) & Eskelin , K. (Participant)
01/01/2021 → 31/12/2024
Project: Other project
Butcher, S. (Project manager), Kajander, T. (Principal Investigator), Lehtiö, L. (Principal Investigator), Määttä, J. A. E. (Principal Investigator), Papageorgiou, A. (Principal Investigator), Rouvinen, J. (Principal Investigator), Salminen, T. (Principal Investigator), Iwai, H. (Principal Investigator), Poranen, M. (Principal Investigator), Eskelin , K. (Participant) & Oksanen, H. M. (Participant)
01/01/2020 → 31/12/2022
Project: Other project
Eskelin , K. (Host)
Activity: Hosting a visitor types › Non-academic visit at UH
Eskelin , K. (Speaker)
Activity: Talk or presentation types › Oral presentation
Oksanen, H. M. (Host), Poranen, M. (Host) & Eskelin , K. (Host)
Activity: Hosting a visitor types › Academic visit at UH
Oksanen, H. M. (Host), Butcher, S. (Host), Poranen, M. (Host) & Eskelin , K. (Host)
Activity: Hosting a visitor types › Academic visit at UH
Eskelin , K. (Reviewer)
Activity: Publication peer-review and editorial work types › Peer review of manuscripts
Poranen, M. M. (Manager), Oksanen, H. M. (Other) & Eskelin, K. J. (Other)
Faculty of Biological and Environmental SciencesFacility/equipment: Core Facility
Butcher, S. (Manager), Oksanen, H. M. (Other), Poranen, M. (Other), Eskelin , K. (Other), Kajander, T. (Other), Permi, P. (Other), Lehtiö, L. (Other), Rouvinen, J. (Other), Salminen, T. A. (Other) & Papageorgiou, A. (Other)
Faculty of Biological and Environmental SciencesFacility/equipment: Coordination office
Butcher, S. (Manager), Oksanen, H. M. (Other), Poranen, M. (Other), Eskelin , K. (Other), Permi, P. (Other), Varjosalo, M. (Other), Lehtiö, L. (Other) & Rouvinen, J. (Other)
Faculty of Biological and Environmental SciencesFacility/equipment: Coordination office