Research Output per year
Personal profile
Description of research and teaching
Novel purification and analysis methods for macromolecular complexes.
Asymmetric flow field-flow fractionation (AF4) is a subtechnique of field-flow fractionation. It separates molecules according to differences in their diffusion coefficients, which in turn reflects their size and shape. AF4 is applicable to a broad range of molecular masses and sizes and molecules with sizes that range from 2 nm to 50 μm can be analyzed.
My research focuses on the development of optimized methods to purify large macromolecular complexes such as ribosomes (a complex of 80 ribosomal proteins and three ribosomal RNAs), viral assemblies as well as intact viruses of various sizes and morphologies. Furthermore, my research aims to use AF4 for detailed analysis of large macromolecular complexes. This can be achieved by analyzing complexes that have been treated with various chemicals, enzymes, temperatures etc.
I am combining the AF4 purification to other methods that include monolithic anion chromatography and centrifugation methods to enable large-scale purification of macromolecular complexes to detailed biochemical and structural analysis.
My previous research included studies on two different plant viruses (Cocksfoot mottle virus and Potato virus A) and applied projects on heterologous protein production in plants.
Fields of Science
- 1183 Plant biology, microbiology, virology
International and National Collaboration
Publications and projects within past five years.
Publications
Asymmetrical flow field-flow fractionation on virus and virus-like particle applications
Eskelin, K., Poranen, M. & Oksanen, H. M., 2019, In : Microorganisms. 7, 11, 555.Research output: Contribution to journal › Review Article › Scientific › peer-review
Open Access
File
Ribosome profiles and riboproteomes of healthy and Potato virus A- and Agrobacterium-infected Nicotiana benthamiana plants
Eskelin, K., Varjosalo, M., Ravantti, J. & Makinen, K., 2019, In : Molecular Plant Pathology. 20, 3, p. 392-409 18 p.Research output: Contribution to journal › Article › Scientific › peer-review
Open Access
File
Asymmetrical flow field-flow fractionation in purification of an enveloped bacteriophage φ 6
Lampi, M., Oksanen, H. M., Meier, F., Moldenhauer, E., Poranen, M. M., Bamford, D. H. & Eskelin, K., 15 Sep 2018, In : Journal of Chromatography. B. 1095, p. 251-257 7 p.Research output: Contribution to journal › Article › Scientific › peer-review
Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4)
Eskelin, K. J. & Poranen, M. M., Nov 2018, In : Viruses. 10, 11, 13 p., 579.Research output: Contribution to journal › Article › Scientific › peer-review
Open Access
File
Halophilic viruses with varying biochemical and biophysical properties are amenable to purification with asymmetrical flow field-flow fractionation
Eskelin, K., Lampi, M., Meier, F., Moldenhauer, E., Bamford, D. H. & Oksanen, H. M., Nov 2017, In : Extremophiles. 21, 6, p. 1119-1132 14 p.Research output: Contribution to journal › Article › Scientific › peer-review
Open Access
File
Projects
- 2 Finished
Kasvivirusinfektion eri vaiheiden ymmärtäminen molekyylitasolla osallistuvien isäntäproteiinien tunnistuksen kautta / rahoitus: Jenny ja Antti Wihurin rahasto
Mäkinen, K., Ivanov, K. I., Lõhmus, A., Eskelin , K., Hafren, A. J. & Basic, M.
09/10/2013 → 31/10/2016
Project: Research project
Towards understanding of the early events of potyvirus biology via synchronous infections.
01/01/2009 → 31/12/2013
Project: Research project
Activities
Asymmetrical flow field-flow fractionation and virus applications
Katri Eskelin (Speaker)24 Feb 2020
Activity: Talk or presentation types › Oral presentation
Journal of Chromatography. A (Journal)
Katri Eskelin (Reviewer)19 Jun 2020
Activity: Publication peer-review and editorial work types › Peer review of manuscripts
20th International Symposium on Field - and Flow-based Separations (FFF2020)
Katri Eskelin (Attendee)23 Feb 2020 → 27 Feb 2020
Activity: Participating in or organising an event types › Organisation and participation in conferences, workshops, courses, seminars
Journal of Chromatography. A (Journal)
Katri Eskelin (Reviewer)20 Apr 2020
Activity: Publication peer-review and editorial work types › Editor of research journal
Viruses (Journal)
Katri Eskelin (Reviewer)27 Apr 2020
Activity: Publication peer-review and editorial work types › Peer review of manuscripts
Equipment
Instruct-HiLIFE Biomolecular Complex Purification Core Facility (Biocomplex)
Minna Marjetta Poranen (Manager), Hanna Maarit Oksanen (Other) & Katri Johanna Eskelin (Other)
Faculty of Biological and Environmental SciencesFacility/equipment: Core Facility