Photo of Katri Eskelin
  • PL 56 (Viikinkaari 9)

    00014

    Finland

  • Viikinkaari 9, Biocenter 1, 6th floor, room 6215

    00014 University of Helsinki

    Finland

20002019

Research output per year

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Personal profile

Description of research and teaching

Novel purification and analysis methods for macromolecular complexes.

Asymmetric flow field-flow fractionation (AF4) is a subtechnique of field-flow fractionation. It separates molecules according to differences in their diffusion coefficients, which in turn reflects their size and shape. AF4 is applicable to a broad range of molecular masses and sizes and molecules with sizes that range from 2 nm to 50 μm can be analyzed.

My research focuses on the development of optimized methods to purify large macromolecular complexes such as ribosomes (a complex of 80 ribosomal proteins and three ribosomal RNAs), viral assemblies as well as intact viruses of various sizes and morphologies. Furthermore, my research aims to use AF4 for detailed analysis of large macromolecular complexes. This can be achieved by analyzing complexes that have been treated with various chemicals, enzymes, temperatures etc.

I am combining the AF4 purification to other methods that include monolithic anion chromatography and centrifugation methods to enable large-scale purification of macromolecular complexes to detailed biochemical and structural analysis.

My previous research included studies on two different plant viruses (Cocksfoot mottle virus and Potato virus A) and applied projects on heterologous protein production in plants.  

Fields of Science

  • 1183 Plant biology, microbiology, virology

International and National Collaboration Publications and projects within past five years.

Publications

Asymmetrical flow field-flow fractionation on virus and virus-like particle applications

Eskelin, K., Poranen, M. & Oksanen, H. M., 2019, In : Microorganisms. 7, 11, 555.

Research output: Contribution to journalReview ArticleScientificpeer-review

Open Access
File
Open Access
File

Asymmetrical flow field-flow fractionation in purification of an enveloped bacteriophage φ 6

Lampi, M., Oksanen, H. M., Meier, F., Moldenhauer, E., Poranen, M. M., Bamford, D. H. & Eskelin, K., 15 Sep 2018, In : Journal of Chromatography. B. 1095, p. 251-257 7 p.

Research output: Contribution to journalArticleScientificpeer-review

Controlled Disassembly and Purification of Functional Viral Subassemblies Using Asymmetrical Flow Field-Flow Fractionation (AF4)

Eskelin, K. J. & Poranen, M. M., Nov 2018, In : Viruses. 10, 11, 13 p., 579.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
File

Halophilic viruses with varying biochemical and biophysical properties are amenable to purification with asymmetrical flow field-flow fractionation

Eskelin, K., Lampi, M., Meier, F., Moldenhauer, E., Bamford, D. H. & Oksanen, H. M., Nov 2017, In : Extremophiles. 21, 6, p. 1119-1132 14 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
File

Projects

Activities

Asymmetrical flow field-flow fractionation and virus applications

Katri Eskelin (Speaker)
24 Feb 2020

Activity: Talk or presentation typesOral presentation

Journal of Chromatography. A (Journal)

Katri Eskelin (Reviewer)
19 Jun 2020

Activity: Publication peer-review and editorial work typesPeer review of manuscripts

20th International Symposium on Field - and Flow-based Separations (FFF2020)

Katri Eskelin (Attendee)
23 Feb 202027 Feb 2020

Activity: Participating in or organising an event typesOrganisation and participation in conferences, workshops, courses, seminars

Journal of Chromatography. A (Journal)

Katri Eskelin (Reviewer)
20 Apr 2020

Activity: Publication peer-review and editorial work typesEditor of research journal

Viruses (Journal)

Katri Eskelin (Reviewer)
27 Apr 2020

Activity: Publication peer-review and editorial work typesPeer review of manuscripts