Research output per year
Research output per year
Personal profile
Description of research and teaching
Novel purification and analysis methods for macromolecular complexes.
Asymmetric flow field-flow fractionation (AF4) is a subtechnique of field-flow fractionation. It separates molecules according to differences in their diffusion coefficients, which in turn reflects their size and shape. AF4 is applicable to a broad range of molecular masses and sizes and molecules with sizes that range from 2 nm to 50 μm can be analyzed.
My research focuses on the development of optimized methods to purify large macromolecular complexes such as ribosomes (a complex of 80 ribosomal proteins and three ribosomal RNAs), viral assemblies as well as intact viruses of various sizes and morphologies. Furthermore, my research aims to use AF4 for detailed analysis of large macromolecular complexes. This can be achieved by analyzing complexes that have been treated with various chemicals, enzymes, temperatures etc.
I am combining the AF4 purification to other methods that include monolithic anion chromatography and centrifugation methods to enable large-scale purification of macromolecular complexes to detailed biochemical and structural analysis.
My previous research included studies on two different plant viruses (Cocksfoot mottle virus and Potato virus A) and applied projects on heterologous protein production in plants.
Fields of Science
- 1183 Plant biology, microbiology, virology
Collaborations and top research areas from the last five years
Recent external collaboration on country/territory level. Dive into details by clicking on the dots or
-
Analysis and purification of ssRNA and dsRNA molecules using asymmetrical flow field flow fractionation
Eskelin , K., Lampi, M., Coustau, C., Imani, J., Kogel, K.-H. & Poranen, M., 8 Nov 2022, In: Journal of Chromatography. A. 1683, 15 p., 463525.Research output: Contribution to journal › Article › Scientific › peer-review
Open AccessFile -
Archaeal viruses: production of virus particles and vesicle-like viruses and purification using asymmetrical flow field-flow fractionation
Eskelin , K. & Oksanen, H. M., 21 Sept 2022, Methods in Molecular Biology - Archaea: methods and protocols. Ferreira-Cerca, S. (ed.). Humana New York, NY, p. 449-465 (Methods in Molecular Biology; vol. 2522).Research output: Chapter in Book/Report/Conference proceeding › Chapter › Scientific › peer-review
Open AccessFile -
Inline-tandem purification of viruses from cell lysate by agarose-based chromatography
Andersson Schönn, M., Eskelin , K., Bamford, D. H., Poranen, M., Unoson, C. & Oksanen, H. M., 1 Mar 2022, In: Journal of Chromatography. B. 1192, 9 p., 123140.Research output: Contribution to journal › Article › Scientific › peer-review
Open AccessFile -
Native RNA purification method for small RNA molecules based on asymmetrical flow field-flow fractionation
Levanova, A., Lampi, M., Kalke, K., Hukkanen, V., Poranen, M. & Eskelin , K., Feb 2022, In: Pharmaceuticals. 15, 2, 22 p., 261.Research output: Contribution to journal › Article › Scientific › peer-review
Open AccessFile -
Isolating, culturing, and purifying viruses with a focus on bacterial and archaeal viruses
Eskelin , K. & Oksanen, H. M., 2021, Encyclopedia of Virology. Zuckerman, M. & Bamford, D. (eds.). 4 ed. Oxford: Academic Press, Vol. 1. p. 162-174Research output: Chapter in Book/Report/Conference proceeding › Entry for encyclopedia / dictionary › Scientific › peer-review
-
Integrated Structural Biology Infrastructure-European Research Infrastructure Consortium (Instruct-ERIC) / Consortium FINStruct and Instruct-ERIC
Butcher, S. (Principal Investigator), Poranen, M. (Co-Principal Investigator), Varjosalo, M. (Principal Investigator), Kajander, T. (Co-Principal Investigator), Oksanen, H. M. (Participant), Eskelin , K. (Participant), Lehtiö, L. (Principal Investigator), Salminen, T. A. (Participant) & Papageorgiou, A. C. (Participant)
01/01/2022 → 31/12/2026
Project: Research Council of Finland: Research infrastructure
-
SusCrop - ERA-NET, Target-specific RNA-based bioprotectants for sustainable crop production in a changing climate
Heinlein, M. (Project manager), Poranen, M. (Project manager), Kogel, K.-H. (Principal Investigator), Patel, A. (Principal Investigator), Eskelin , K. (Participant), Korhonen, S. M. (Participant), Levanova, A. (Participant), Westerholm, T. T. (Participant) & Zaytseva, A. (Participant)
01/06/2021 → 31/05/2025
Project: Ministry funding
-
Structural Biology Finland (FINStruct) & Instruct-ERIC Centre FI / Research Infrastructure Roadmap 2021-2024
Butcher, S. (Principal Investigator), Poranen, M. (Co-Principal Investigator), Kajander, T. (Co-Principal Investigator), Iwai, H. (Co-Principal Investigator), Varjosalo, M. (Co-Principal Investigator), Lehtiö, L. (Co-Principal Investigator), Rouvinen, J. (Co-Principal Investigator), Salminen, T. A. (Co-Principal Investigator), Papageorgiou, A. C. (Co-Principal Investigator), Oksanen, H. M. (Participant) & Eskelin , K. (Participant)
01/01/2021 → 31/12/2024
Project: Research Council of Finland: Research infrastructure
-
Instruct-HiLIFE: Integrated structural cell biology research infrastructure platform, HiLIFE, University of Helsinki
Poranen, M. (Project manager), Butcher, S. (Principal Investigator), Iwai, H. (Principal Investigator), Kajander, T. (Principal Investigator), Oksanen, H. M. (Participant) & Eskelin , K. (Participant)
01/01/2021 → 31/12/2024
Project: Other project
-
Integrated Structural Biology Infrastructure / Consortium: Instruct-Suomi
Butcher, S. (Project manager), Kajander, T. (Principal Investigator), Lehtiö, L. (Principal Investigator), Määttä, J. A. E. (Principal Investigator), Papageorgiou, A. (Principal Investigator), Rouvinen, J. (Principal Investigator), Salminen, T. (Principal Investigator), Iwai, H. (Principal Investigator), Poranen, M. (Principal Investigator), Eskelin , K. (Participant) & Oksanen, H. M. (Participant)
01/01/2020 → 31/12/2022
Project: Other project
Activities
-
Robert Mildner
Eskelin , K. (Host)
26 Sept 2022 → 28 Sept 2022Activity: Hosting a visitor types › Non-academic visit at UH
-
Structural Biology Platform FINStruct and Instruct-ERIC Centre Finland 2022 Annual Meeting, November 30th, 2022 in
Eskelin , K. (Speaker)
30 Nov 2022Activity: Talk or presentation types › Oral presentation
-
Miguel Cantero Riviejo
Oksanen, H. M. (Host), Poranen, M. (Host) & Eskelin , K. (Host)
Jan 2022 → Jul 2022Activity: Hosting a visitor types › Academic visit at UH
-
Catarina Paiva
Oksanen, H. M. (Host), Butcher, S. (Host), Poranen, M. (Host) & Eskelin , K. (Host)
Aug 2022 → Nov 2022Activity: Hosting a visitor types › Academic visit at UH
-
Membranes (Journal)
Eskelin , K. (Reviewer)
Oct 2022Activity: Publication peer-review and editorial work types › Peer review of manuscripts
Equipment
-
Biomolecular Complex Purification Facility (LS-RIA/ Instruct-HiLIFE)
Poranen, M. M. (Manager), Oksanen, H. M. (Other) & Eskelin, K. J. (Other)
Faculty of Biological and Environmental SciencesFacility/equipment: Core Facility
-
FINStruct Structural Biology Finland
Butcher, S. (Manager), Oksanen, H. M. (Other), Poranen, M. (Other), Eskelin , K. (Other), Kajander, T. (Other), Permi, P. (Other), Lehtiö, L. (Other), Rouvinen, J. (Other), Salminen, T. A. (Other) & Papageorgiou, A. (Other)
Faculty of Biological and Environmental SciencesFacility/equipment: Coordination office
-
Instruct-ERIC Structural Biology European Research Infrastructure Centre Finland
Butcher, S. (Manager), Oksanen, H. M. (Other), Poranen, M. (Other), Eskelin , K. (Other), Permi, P. (Other), Varjosalo, M. (Other), Lehtiö, L. (Other) & Rouvinen, J. (Other)
Faculty of Biological and Environmental SciencesFacility/equipment: Coordination office