Photo of Maria Pajunen

Maria Pajunen

Adjunct professor in Molecular Microbiology

  • PL 21 (Haartmaninkatu 3)

    00014

    Finland

20002018
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Publications 2000 2018

2018

Mu transpososome activity-profiling yields hyperactive MuA variants for highly efficient genetic and genome engineering

Rasila, T. S., Pulkkinen, E., Kiljunen, S., Haapa-Paananen, S., Pajunen, M. I., Salminen, A., Paulin, L., Vihinen, M., Rice, P. A. & Savilahti, H., 18 May 2018, In : Nucleic Acids Research. 46, 9, p. 4649-4661 12 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
File
2017

Internalization of a polysialic acid-binding Escherichia coli bacteriophage into eukaryotic neuroblastoma cells

Lehti, T. A., Pajunen, M. I., Skog, M. S. & Finne, J., 4 Dec 2017, In : Nature Communications. 8, 12 p., 1915.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
File

Several Hfq-dependent alterations in physiology of Yersinia enterocolitica O: 3 are mediated by derepression of the transcriptional regulator RovM

Leskinen, K., Pajunen, M. I., Varjosalo, M., Fernandez-Carrasco, H., Bengoechea, J. A. & Skurnik, M., Mar 2017, In : Molecular Microbiology. 103, 6, p. 1065-1091 27 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access

Transposon insertion mutagenesis for archaeal gene discovery

Kiljunen, S. J., Pajunen, M. I. & Savilahti, H. T., 2017, In Vitro Mutagenesis: Methods and Protocols, Methods in Molecular Biology. Reeves, A. (ed.). Springer , Vol. 1498. p. 309-320 11 p.

Research output: Chapter in Book/Report/Conference proceedingChapterScientificpeer-review

2016

Expression of neural cell adhesion molecule and polysialic acid in human bone marrow-derived mesenchymal stromal cells

Skog, M. S., Nystedt, J., Korhonen, M., Anderson, H., Lehti, T. A., Pajunen, M. I. & Finne, J., 2016, In : Stem Cell Research & Therapy. 7, 12 p., 113.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
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Yersinia enterocolitica-Specific Infection by Bacteriophages TG1 and phi R1-RT Is Dependent on Temperature-Regulated Expression of the Phage Host Receptor OmpF

Leon-Velarde, C. G., Happonen, L., Pajunen, M., Leskinen, K., Kropinski, A. M., Mattinen, L., Rajtor, M., Zur, J., Smith, D., Chen, S., Nawaz, A., Johnson, R. P., Odumeru, J. A., Griffiths, M. W. & Skurnik, M., Sep 2016, In : Applied and Environmental Microbiology. 82, 17, p. 5340-5353 14 p.

Research output: Contribution to journalArticleScientificpeer-review

2014

Generation of comprehensive transposon insertion mutant library for the model archaeon, Haloferax volcanii, and its use for gene discovery

Kiljunen, S., Pajunen, M. I., Dilks, K., Storf, S., Pohlschroder, M. & Savilahti, H., 9 Dec 2014, In : BMC Biology. 12, 14 p., 103.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
File
2012

Construction and Screening of the Transposon Insertion Library of Yersinia enterocolitica (YeO3-R1)

Pajunen, M., Pinta, E. M. & Skurnik, M., 2012, (Bio-Protocol)

Research output: Book/ReportBookProfessional

Identification of three oligo-/polysaccharide-specific ligases in Yersinia enterocolitica: Y. enterocolitica oligo-/polysaccharide ligases

Pinta, E. M., Li, Z., Batzilla, J., Pajunen, M., Kasanen, T., Rabsztyn, K., Rakin, A. & Skurnik, M., Jan 2012, In : Molecular Microbiology. 83, 1, p. 125-136 12 p.

Research output: Contribution to journalArticleScientificpeer-review

Metabolism of Vertebrate Amino Sugars with N-Glycolyl Groups: Resistance of α-2-8-linked N-glycolylneuraminic acid to enzymatic cleavage

Davies, L. R. L., Pearce, O. M. T., Tessier, M. B., Assar, S., Smutova, V., Pajunen, M., Sumida, M., Sato, C., Kitajima, K., Finne, J., Gagneux, P., Pshezhetsky, A., Woods, R. & Varki, A., 17 Aug 2012, In : Journal of Biological Chemistry. 287, 34, p. 28917-28931 15 p.

Research output: Contribution to journalArticleScientificpeer-review

2010

Mso1p Regulates Membrane Fusion through Interactions with the Putative N-Peptide-binding Area in Sec1p Domain 1

Weber, M., Chernov, K., Turakainen, H., Wohlfahrt, G., Pajunen, M., Savilahti, H. & Jantti, J., 2010, In : Molecular Biology of the Cell. 21, 8, p. 1362-1374 13 p.

Research output: Contribution to journalArticleScientificpeer-review

Universal platform for quantitative analysis of DNA transposition

Pajunen, M., Rasila, T., Happonen, L., Lamberg, A., Haapa-Paananen, S., Kiljunen, S. J. & Savilahti, H., 2010, In : Mobile DNA. 1, 24, 14 p.

Research output: Contribution to journalArticleScientificpeer-review

2009

Application of Mu in vitro transposition for high-precision mapping of protein-protein interfaces on a yeast two-hybrid platform

Pajunen, M., Poussu, E., Turakainen, H. & Savilahti, H., 2009, In : Methods. 49, 3, p. 255-262 8 p.

Research output: Contribution to journalArticleScientificpeer-review

Critical evaluation of random mutagenesis by error-prone polymerase chain reaction protocols, Escherichia coli mutator strain, and hydroxylamine treatment

Rasila, T. S., Pajunen, M. I. & Savilahti, H., 2009, In : Analytical Biochemistry. 388, 1, p. 71-80 10 p.

Research output: Contribution to journalArticleScientificpeer-review

Generation of single-copy transposon insertions in Clostridium perfringens by electroporation of phage mu DNA transposition complexes

Lanckriet, A., Timbermont, L., Happonen, L. J., Pajunen, M., Pasmans, F., Haesebrouck, F., Ducatelle, R., Savilahti, H. & Van Immerseel, F., 2009, In : Applied and Environmental Microbiology. 75, 9, p. 2638-2642 5 p.

Research output: Contribution to journalArticleScientificpeer-review

2008

Bacteriophage Mu integration in yeast and mammalian genomes

Paatero, A. O., Turakainen, H., Happonen, L. J., Olsson, C., Palomäki, T., Pajunen, M. I., Meng, X., Otonkoski, T., Tuuri, T., Berry, C., Malani, N., Frilander, M. J., Bushman, F. & Savilahti, H., 2008, In : Nucleic Acids Research. 36, 22, p. e148 1 p.

Research output: Contribution to journalArticleScientificpeer-review

Phage therapy

Skurnik, M., Kiljunen, S. & Pajunen, M., 2008, Therapeutic Microbiology: Probiotics and Related Strategies. Versalovic, J. & Wilson, M. (eds.). Washington, DC: ASM Press, p. 373-389 17 p.

Research output: Chapter in Book/Report/Conference proceedingChapterScientificpeer-review

Phage Therapy

Skurnik, M., Kiljunen, S. J. & Pajunen, M., 2008, Therapeutic Microbiology: Probiotics and Related Strategies. Versalovic, J. & Wilson, M. (eds.). 1st ed. ed. Washington, DC: ASM Press, p. 373-389 16 p.

Research output: Chapter in Book/Report/Conference proceedingChapterScientificpeer-review

2007

Biotechnological challenges of phage therapy

Skurnik, M., Pajunen, M. & Kiljunen, S., 2007, In : Biotechnology Letters. 29, 7, p. 995-1003 9 p.

Research output: Contribution to journalArticleScientificpeer-review

High-precision mapping of protein-protein interfaces: an integrated genetic strategy combining en masse mutagenesis and DNA-level parallel analysis on a yeast two-hybrid platform

Pajunen, M., Turakainen, H., Poussu, E., Peränen, J., Vihinen, M. & Savilahti, H., 2007, In : Nucleic Acids Research. 35, 16, e103, [11] s

Research output: Contribution to journalArticleScientificpeer-review

Isolation and characterization of biofilm formation-defective mutants of Staphylococcus aureus

Tu Quoc, P. H., Genevaux, P., Pajunen, M., Savilahti, H. & Georgopoulos, C., 2007, In : Infection and Immunity. 75, 3, p. 1079-1088 10 p.

Research output: Contribution to journalArticleScientificpeer-review

SNP discovery by mismatch-targeting of Mu transposition

Orsini, L., Pajunen, M., Hanski, I. & Savilahti, H., 2007, In : Nucleic Acids Research. 35, 6, s

Research output: Contribution to journalArticleScientificpeer-review

2005

Generation of transposon insertion mutant libraries for Gram-positive bacteria by electroporation of phage Mu DNA transposition complexes

Pajunen, M. I., Pulliainen, A. T., Finne, J. & Savilahti, H., 2005, In : Microbiology. 151, p. 1209-1218 10 p.

Research output: Contribution to journalArticleScientificpeer-review

Nonessential genes of phage [phi]YeO3-12 include genes involved in adaption to growth on Yersinia enterocolitica serotype O:3

Kiljunen, S., Vilen, H., Pajunen, M., Savilahti, H. & Skurnik, M., 2005, In : Journal of Bacteriology. 187, 4, p. 1405-1414 10 p.

Research output: Contribution to journalArticleScientificpeer-review

Nonessential Genes of Phage φYeO3-12 Include Genes Involved in Adaptation to Growth on Yersinia enterocolitica Serotype O:3

Kiljunen, S., Vilen, H., Pajunen, M., Savilahti, H. & Skurnik, M., 2005, In : Journal of Bacteriology. 187, 4, p. 1405-1414 10 p.

Research output: Contribution to journalArticleScientificpeer-review

Novel mutants of Erwinia carotovora subsp. carotovora defective in the production of plant cell wall degrading enzymes generated by Mu transpososome-mediated insertion mutagenesis

Laasik, E., Ojarand, M., Pajunen, M., Savilahti, H. & Mäe, A., 2005, In : FEMS Microbiology Letters. 243, p. 93-99 7 p.

Research output: Contribution to journalArticleScientificpeer-review

2003

Yersiniophages - Special reference to phi YeO3-12

Pajunen, M. I., Molineux, I. J. & Skurnik, M., 2003, The genus yersinia: entering the functional genomic era. Skurnik, M., Granfors, K. & Bengoechea, J. A. (eds.). Kluwer Academic, p. 233-240 8 p. (Advances in experimental medicine and biology; no. 529).

Research output: Chapter in Book/Report/Conference proceedingConference contributionScientific

2002

Complete nucleotide sequence and likely recombinatorial origin of bacteriophage T3

Pajunen, M. I., Elizondo, M. R., Skurnik, M., Kieleczawa, J. & Molineux, I. J., 2002, In : Journal of Molecular Biology. 319, 5, p. 1115-1132 18 p.

Research output: Contribution to journalArticleScientificpeer-review

Food associated listeriosis, yersiniosis, and botulism: new DNA-based methods for studying the infection routes, controlling the spread and assessing the risks in food production

Korkeala, H., Asplund, K., Autio, T., Björkroth, J., Fredriksson-Ahomaa, M., Hellström, S., Hielm, S., Hyytiä, E., Jalava, K., Korte, T., Lindström, M., Lundén, J., Markkula, A., Nevas, M., Miettinen, M., Oksa, L., Siitonen, A., Hallanvuo, S., Lukinmaa, S., Rinttilä, T. & 4 othersSkurnik, M., Kiljunen, S., Pajunen, M. & Söderholm, L., 2002, SYTTY Finnish Research Programme on Environmental Health 1998-2001: Results. Juuti, S. & Leinonen, H. (eds.). Kuopio: Finnish Research Programme on Environmental Health, p. 276-285 10 p. (Publications of the Finnish Research Programme on Environmental Health - SYTTY; no. 1/2002).

Research output: Chapter in Book/Report/Conference proceedingChapterScientific

2001

Complete Genomic Sequence of the Lytic Bacteriophage φYeO3-12 of Yersinia enterocolitica Serotype O:3

Pajunen, M. I., Kiljunen, S. J., Söderholm, M. E. -L. & Skurnik, M., 2001, In : Journal of Bacteriology. 183, 6, p. 1928-1937 10 p.

Research output: Contribution to journalArticleScientificpeer-review

2000

Bacteriophage φYeO3-12, Specific forYersinia enterocolitica Serotype O:3, Is Related to Coliphages T3 and T7

Pajunen, M., Kiljunen, S. & Skurnik, M., 2000, In : Journal of Bacteriology. 182, 18, p. 5114-5120 7 p.

Research output: Contribution to journalArticleScientificpeer-review