Photo of Matjaz Barboric
  • PL 63 (Haartmaninkatu 8)

    00014

    Finland

19992016
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Personal profile

Description of research and teaching

Controlling Transcription Elongation by RNA polymerase II: mechanisms, disease and development

We are investigating the fundamental mechanisms of metazoan gene transcription by RNA polymerase II (RNAPII). In particular, we are studying the regulatory factors that modulate RNAPII transcription at the level of elongation, which has been recognized only recently as a major rate-limiting step in gene expression. Our work is focused mainly on the key cyclin-dependent kinases that stimulate RNAPII elongation and phosphorylate the C-terminal domain of the biggest subunit of RNAPII, Rbp1, at serine 2 (Ser2-P CTD). By using biochemistry, genetics and the tools of systems biology, we are aiming to understand mechanisms that enable the function of elongation kinases as well as biological processes that are controlled by them. Because transcription elongation can be intimately connected to pre-mRNA processing in the course of transcription via Ser2-P CTD, we are also studying how elongation kinases affect mRNA maturation events such as alternative splicing. Of note, an important aspect of our work is to uncover how deregulation of RNAPII elongation control contributes to the onset of deadly diseases, including cancer. Finally, revealing the significance of transcription elongation kinases in embryonic development remains a pressing issue in our research group.

Keywords

  • 118 Biological sciences
  • gene expression
  • transcription elongation
  • P-TEFb
  • 7SK snRNP
  • pre-mRNA splicing
  • HIV-1 gene regulation

International and National Collaboration Publications and projects within past five years.

Publications 1999 2016

Cracking the control of RNA polymerase II elongation by 7SK snRNP and P-TEFb

Christiano Quaresma, A. J., Bugai, A. & Barboric, M., 19 Sep 2016, In : Nucleic Acids Research. 44, 16, p. 7527-7539 13 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
File

Influenza virus NS1 protein binds cellular DNA to block transcription of antiviral genes

Anastasina, M., Le May, N., Bugai, A., Fu, Y., Soderholm, S., Gaelings, L., Ohman, T., Tynell, J., Kyttanen, S., Barboric, M., Nyman, T. A., Matikainen, S., Julkunen, I., Butcher, S. J., Egly, J-M. & Kainov, D. E., Nov 2016, In : Biochimica et Biophysica Acta. Gene Regulatory Mechanisms. 1859, 11, p. 1440-1448 9 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
File

The two sides of Tat

Barboric, M. & Fujinaga, K., 19 Jan 2016, In : eLife. 5, 3 p., 12686.

Research output: Contribution to journalArticleScientific

Open Access
File

Ovarian carcinoma CDK12 mutations misregulate expression of DNA repair genes via deficient formation and function of the Cdk12/CycK complex

Ekumi, K., Paculova, H., Lenasi, T., Pospichalova, V., Boesken, C. A., Rybarikova, J., Bryja, V., Geyer, M., Blazek, D. & Barboric, M., 11 Mar 2015, In : Nucleic Acids Research. 43, 5, p. 2575-2589 15 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
File

Mutual relationships between transcription and pre-mRNA processing in the synthesis of mRNA

Lenasi, T. & Barboric, M., 2013, In : Wiley Interdisciplinary Reviews. RNA. 4, 2, p. 139-154 16 p.

Research output: Contribution to journalReview ArticleScientificpeer-review

Projects 2011 2014

Sigrid Juseliuksen Säätiö/Barboric Matjaz 2011

Barboric, M.

Unknown funder

01/06/201130/04/2014

Project: Research project