Projects per year
Collaboration: Research Professor Kristiina Kruus, Dr. Harry Boer, VTT, Finland;
FiDiPro Fellow Emma Master, Aalto University, Finland / University of Toronro, Canada
The aim of the ENOX-project is to develop novel oxidative enzymes to selectively create reactive carbonyl and carboxyl functionalities within oligo- and polysaccharides. The target enzymes do not require expensive coenzymes and are able to combine the cheap and clean oxidant O2 with a high specificity and regioselectivity. The work will cover different enzyme types acting specifically on various hydroxyl groups in carbohydrates. The project is divided in four workpackages: 1. Substrate and assay development for HTP screening, 2. Screening of new carbohydrate-acting oxidases, 3. Development of new oxidases through protein engineering, 4. Evaluation of action and selective oxidation of oligo- and polysaccharides, and fibres, which will be carried out in a close collaboration of the consortium partners. The project will result in new biocatalysts for targeted modifications of polysaccharides and fibres, and will contribute to the future refining of biobased polymers to novel products.
|Effective start/end date||01/09/2011 → 31/08/2014|
Fields of Science
- 220 Industrial biotechnology
- 116 Chemical sciences
- 2 Finished
Production of aldehydes and functionalized polysaccharides by galactose oxidase and chemical modifications
01/01/2010 → 31/12/2012
Project: Research project
01/01/2007 → 31/12/2010
Project: Research project
- 3 Article
A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 familyMollerup, F., Aumala, V., Parikka, K. M., Mathieu, Y., Brumer, H., Tenkanen, T. M. & Master, E., 15 May 2019, In: PLoS One. 14, 5, p. 1-19 19 p., 0216546.
Research output: Contribution to journal › Article › Scientific › peer-reviewOpen AccessFile
Andberg, M., Mollerup, F., Parikka, K. M., Koutaniemi, S. M., Boer, H., Juvonen, M. K., Master, E., Tenkanen, T. M. & Kruus, K., 2017, In: Applied and Environmental Microbiology. 83, 20, 17 p., 01383-17.
Research output: Contribution to journal › Article › Scientific › peer-reviewOpen Access
Influence of a family 29 carbohydrate binding module on the activity of galactose oxidase from Fusarium graminearumMollerup, F., Parikka, K. M., Tenkanen, T. M. & Master, E., 2016, In: Biochimica et Biophysica Acta. General Subjects. 1860, 2, p. 354-362 8 p.
Research output: Contribution to journal › Article › Scientific › peer-review