Phenotypic biosensor-based HTS and mode of action analysis by metabolomics and transcriptomics for enhancing antimicrobial drug discovery against Gram-negative bacteria

Project Details

Description

Infectious diseases are still one of the leading causes of morbidity and mortality worldwide, and the need for new antimicrobials is evident. Novel compounds with potential antimicrobial properties are continuously discovered, for example from natural products, but in many cases found activities are not being characterised beyond the initial discovery phase. This is most of all due to the lack of methods for elucidating the mode of action (MoA). We aim to develop predictive tools for pathway and MoA elucidation and for follow-up studies, and to validate the overall process by studying compounds with unknown MoA. The active agents potentially identified may provide experimental tools or antimicrobial lead compounds for applications for human and/or animal use. Importantly, the project will provide fast and efficient tools to carry initial findings forward towards becoming truly significant new antimicrobial compounds for the fight against microbial diseases.
StatusFinished
Effective start/end date01/09/201431/08/2019

Fields of Science

  • 317 Pharmacy

Research Output

2-Aminopyridine Analogs Inhibit Both Enzymes of the Glyoxylate Shunt in Pseudomonas aeruginosa

McVey, A., Bartlett, S., Kajbaf, M., Pellacani, A., Gatta, V., Tammela, P., Spring, D. R. & Welch, M., 3 Apr 2020, In : International Journal of Molecular Sciences. 21, p. 2490 16 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access

A new cell-based AI-2-mediated quorum sensing interference assay in screening of LsrK-targeted inhibitors

Gatta, V., Tomašič, T., Ilaš, J., Zidar, N., Peterlin Mašič, L., Barančoková, M., Frlan, R., Anderluh, M., Kikelj, D. & Tammela, P., 6 Feb 2020, In : ChemBioChem : a European journal of chemical biology.

Research output: Contribution to journalArticleScientificpeer-review

An optimised series of substituted N-phenylpyrrolamides as DNA gyrase B inhibitors

Benetto Tiz, D., Skok, Ž., Durcik, M., Tomašič, T., Peterlin Mašič, L., Ilaš, J., Draskovits, G., Révész, T., Nyerges, Á., Pál, C., Cruz, C. D., Tammela, P. S. M., Žigon, D., Kikelj, D. & Zidar, N., 1 Apr 2019, In : European Journal of Medicinal Chemistry. 167, p. 269-290 22 p.

Research output: Contribution to journalArticleScientificpeer-review

Open Access
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Activities

Supervision of ERASMUS student (University of Barcelona, Spain)

Polina Ilina (Teacher), CD Cruz (Teacher), Päivi Tammela (Teacher)
12 Sep 201831 Jan 2019

Activity: Other activity typesTypes for other activities - External teaching and subject coordination

Elisa Sassetti

Päivi Tammela (Host)
2 May 20181 Jun 2018

Activity: Hosting a visitor typesAcademic visit at UH

Supervision of ERASMUS student (D'Annunzio University of Chieti-Pescara, Italy)

CD Cruz (Teacher), Päivi Tammela (Teacher)
8 Oct 201818 Dec 2018

Activity: Other activity typesTypes for other activities - External teaching and subject coordination