We aim at discovering new antimicrobial agents from active waste process sites. We hypothesize that natural compounds of bacterial origin at these sites bear significant antimicrobial activity harnessable for fight against the global antimicrobial resistance. We focus on combating multidrug-resistant, biofilm- forming Staphylococcus aureus. First, waste process sites will be screened for their bacterial activity by using biofilm sensors. The bacteria-active sites will be identified and sampled for isolation of bacterial cells on microwell arrays. The arrays will be brought back to the original active waste process site, to induce cell growth on the microwells. After cell culturing, the arrays will be brought back to laboratory for further testing of bacteria, including microbial identification and dereplication of the chemical profile of crude extracts. Last, the new antibiotic compounds will be identified and their derivatives synthesized prior to their functional characterization.
|Effective start/end date||01/01/2017 → 31/12/2020|