A baseline study of vicine–convicine levels in faba bean (Vicia faba L.) germplasm

Khalil Khamassi, Faycal Ben Jeddi, Doug Hobbs, Jose Irigoyen, Fred Stoddard, Donal M O'Sullivan, Huw Jones

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Vicine and convicine are anti-nutritional compounds that accumulate in the cotyledons of faba beans. When humans consume beans with high levels of these compounds, it can cause a condition called favism in individuals harbouring a deficiency in the activity of their glucose-6-phosphate dehydrogenase. When faba beans are used in animal feeds, there can be effects on performance. These concerns have resulted in increasing interest within plant breeding in developing low vicine and convicine faba bean germplasm. In order to facilitate this objective, we developed a rapid and robust screening method for vicine and convicine, capable of distinguishing between faba beans that are either high (wild type) or low in vicine and convicine. In the absence of reliable commercial reference materials, we report an adaptation of a previously published method where a biochemical assay and spectral data were used to confirm the identity of our analytes, vicine and convicine. This method could be readily adopted in other facilities and open the way to the efficient exploitation of diverse germplasm in regions where faba beans play a significant role in human nutrition. We screened a collection of germplasm of interest to a collaborative plant breeding rogramme
developing between the National Institute for Agricultural Botany in the UK and L’Institut Nationale d’Agronomie de Tunisie in Tunisia. We report the results obtained and discuss the prospects for developing molecular markers for the low vicine and convicine trait.
Original languageEnglish
JournalPlant Genetic Resources
Volume11
Issue number03
Pages (from-to)250-257
Number of pages8
ISSN1479-2621
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 4111 Agronomy
  • faba bean
  • HPLC
  • Vicia
  • vicine-convicine

Cite this

Khamassi, K., Ben Jeddi, F., Hobbs, D., Irigoyen, J., Stoddard, F., O'Sullivan, D. M., & Jones, H. (2013). A baseline study of vicine–convicine levels in faba bean (Vicia faba L.) germplasm. Plant Genetic Resources, 11(03), 250-257. https://doi.org/10.1017/S1479262113000105
Khamassi, Khalil ; Ben Jeddi, Faycal ; Hobbs, Doug ; Irigoyen, Jose ; Stoddard, Fred ; O'Sullivan, Donal M ; Jones, Huw. / A baseline study of vicine–convicine levels in faba bean (Vicia faba L.) germplasm. In: Plant Genetic Resources. 2013 ; Vol. 11, No. 03. pp. 250-257.
@article{db8e6292724842979232feaaf91534b7,
title = "A baseline study of vicine–convicine levels in faba bean (Vicia faba L.) germplasm",
abstract = "Vicine and convicine are anti-nutritional compounds that accumulate in the cotyledons of faba beans. When humans consume beans with high levels of these compounds, it can cause a condition called favism in individuals harbouring a deficiency in the activity of their glucose-6-phosphate dehydrogenase. When faba beans are used in animal feeds, there can be effects on performance. These concerns have resulted in increasing interest within plant breeding in developing low vicine and convicine faba bean germplasm. In order to facilitate this objective, we developed a rapid and robust screening method for vicine and convicine, capable of distinguishing between faba beans that are either high (wild type) or low in vicine and convicine. In the absence of reliable commercial reference materials, we report an adaptation of a previously published method where a biochemical assay and spectral data were used to confirm the identity of our analytes, vicine and convicine. This method could be readily adopted in other facilities and open the way to the efficient exploitation of diverse germplasm in regions where faba beans play a significant role in human nutrition. We screened a collection of germplasm of interest to a collaborative plant breeding rogrammedeveloping between the National Institute for Agricultural Botany in the UK and L’Institut Nationale d’Agronomie de Tunisie in Tunisia. We report the results obtained and discuss the prospects for developing molecular markers for the low vicine and convicine trait.",
keywords = "4111 Agronomy, faba bean, HPLC, Vicia, vicine-convicine",
author = "Khalil Khamassi and {Ben Jeddi}, Faycal and Doug Hobbs and Jose Irigoyen and Fred Stoddard and O'Sullivan, {Donal M} and Huw Jones",
year = "2013",
doi = "10.1017/S1479262113000105",
language = "English",
volume = "11",
pages = "250--257",
journal = "Plant Genetic Resources",
issn = "1479-2621",
publisher = "Cambridge University Press",
number = "03",

}

Khamassi, K, Ben Jeddi, F, Hobbs, D, Irigoyen, J, Stoddard, F, O'Sullivan, DM & Jones, H 2013, 'A baseline study of vicine–convicine levels in faba bean (Vicia faba L.) germplasm' Plant Genetic Resources, vol. 11, no. 03, pp. 250-257. https://doi.org/10.1017/S1479262113000105

A baseline study of vicine–convicine levels in faba bean (Vicia faba L.) germplasm. / Khamassi, Khalil; Ben Jeddi, Faycal; Hobbs, Doug; Irigoyen, Jose; Stoddard, Fred; O'Sullivan, Donal M; Jones, Huw.

In: Plant Genetic Resources, Vol. 11, No. 03, 2013, p. 250-257.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A baseline study of vicine–convicine levels in faba bean (Vicia faba L.) germplasm

AU - Khamassi, Khalil

AU - Ben Jeddi, Faycal

AU - Hobbs, Doug

AU - Irigoyen, Jose

AU - Stoddard, Fred

AU - O'Sullivan, Donal M

AU - Jones, Huw

PY - 2013

Y1 - 2013

N2 - Vicine and convicine are anti-nutritional compounds that accumulate in the cotyledons of faba beans. When humans consume beans with high levels of these compounds, it can cause a condition called favism in individuals harbouring a deficiency in the activity of their glucose-6-phosphate dehydrogenase. When faba beans are used in animal feeds, there can be effects on performance. These concerns have resulted in increasing interest within plant breeding in developing low vicine and convicine faba bean germplasm. In order to facilitate this objective, we developed a rapid and robust screening method for vicine and convicine, capable of distinguishing between faba beans that are either high (wild type) or low in vicine and convicine. In the absence of reliable commercial reference materials, we report an adaptation of a previously published method where a biochemical assay and spectral data were used to confirm the identity of our analytes, vicine and convicine. This method could be readily adopted in other facilities and open the way to the efficient exploitation of diverse germplasm in regions where faba beans play a significant role in human nutrition. We screened a collection of germplasm of interest to a collaborative plant breeding rogrammedeveloping between the National Institute for Agricultural Botany in the UK and L’Institut Nationale d’Agronomie de Tunisie in Tunisia. We report the results obtained and discuss the prospects for developing molecular markers for the low vicine and convicine trait.

AB - Vicine and convicine are anti-nutritional compounds that accumulate in the cotyledons of faba beans. When humans consume beans with high levels of these compounds, it can cause a condition called favism in individuals harbouring a deficiency in the activity of their glucose-6-phosphate dehydrogenase. When faba beans are used in animal feeds, there can be effects on performance. These concerns have resulted in increasing interest within plant breeding in developing low vicine and convicine faba bean germplasm. In order to facilitate this objective, we developed a rapid and robust screening method for vicine and convicine, capable of distinguishing between faba beans that are either high (wild type) or low in vicine and convicine. In the absence of reliable commercial reference materials, we report an adaptation of a previously published method where a biochemical assay and spectral data were used to confirm the identity of our analytes, vicine and convicine. This method could be readily adopted in other facilities and open the way to the efficient exploitation of diverse germplasm in regions where faba beans play a significant role in human nutrition. We screened a collection of germplasm of interest to a collaborative plant breeding rogrammedeveloping between the National Institute for Agricultural Botany in the UK and L’Institut Nationale d’Agronomie de Tunisie in Tunisia. We report the results obtained and discuss the prospects for developing molecular markers for the low vicine and convicine trait.

KW - 4111 Agronomy

KW - faba bean

KW - HPLC

KW - Vicia

KW - vicine-convicine

U2 - 10.1017/S1479262113000105

DO - 10.1017/S1479262113000105

M3 - Article

VL - 11

SP - 250

EP - 257

JO - Plant Genetic Resources

JF - Plant Genetic Resources

SN - 1479-2621

IS - 03

ER -