A comparative map of macroautophagy and mitophagy in the vertebrate eye

Thomas McWilliams, Prescott Alan R., Beatriz Villarejo-Zori, Graeme Ball, Patricia Boya, Ian G. Ganley

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Photoreception is pivotal to our experience and perception of the natural world; hence the eye is of
prime importance for most vertebrate animals to sense light. Central to visual health is mitochondrial
homeostasis, and the selective autophagic turnover of mitochondria (mitophagy) is predicted to play
a key role here. Despite studies that link aberrant mitophagy to ocular dysfunction, little is known about
the prevalence of basal mitophagy, or its relationship to general autophagy, in the visual system. In this
study, we utilize the mito-QC mouse and a closely related general macroautophagy reporter model to
profile basal mitophagy and macroautophagy in the adult and developing eye. We report that ocular
macroautophagy is widespread, but surprisingly mitophagy does not always follow the same pattern of
occurrence. We observe low levels of mitophagy in the lens and ciliary body, in stark contrast to the high
levels of general MAP1LC3-dependent macroautophagy in these regions. We uncover a striking reversal
of this process in the adult retina, where mitophagy accounts for a larger degree of the macroautophagy
taking place, specifically in the photoreceptor neurons of the outer nuclear layer. We also show the
developmental regulation of autophagy in a variety of ocular tissues. In particular, mitophagy in the
adult mouse retina is reversed in localization during the latter stages of development. Our work thus
defines the landscape of mitochondrial homeostasis in the mammalian eye, and in doing so highlights
the selective nature of autophagy in vivo and the specificity of the reporters used.
Original languageEnglish
JournalAutophagy
Pages (from-to)1
Number of pages13
ISSN1554-8627
DOIs
Publication statusPublished - 21 Feb 2019
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1184 Genetics, developmental biology, physiology
  • 1182 Biochemistry, cell and molecular biology
  • 3111 Biomedicine
  • 3112 Neurosciences

Cite this

McWilliams, Thomas ; Alan R., Prescott ; Villarejo-Zori, Beatriz ; Ball, Graeme ; Boya, Patricia ; Ganley, Ian G. / A comparative map of macroautophagy and mitophagy in the vertebrate eye. In: Autophagy. 2019 ; pp. 1.
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A comparative map of macroautophagy and mitophagy in the vertebrate eye. / McWilliams, Thomas; Alan R., Prescott; Villarejo-Zori, Beatriz ; Ball, Graeme; Boya, Patricia; Ganley, Ian G.

In: Autophagy, 21.02.2019, p. 1.

Research output: Contribution to journalArticleScientificpeer-review

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