A comparison of methods to estimate photosynthetic light absorption in leaves with contrasting morphology

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Accurate temporal and spatial measurements of leaf optical traits (i.e., absorption, reflectance and transmittance) are paramount to photosynthetic studies. These optical traits are also needed to couple radiative transfer and physiological models to facilitate the interpretation of optical data. However, estimating leaf optical traits in leaves with complex morphologies remains a challenge. Leaf optical traits can be measured using integrating spheres, either by placing the leaf sample in one of the measuring ports (External Method) or by placing the sample inside the sphere (Internal Method). However, in leaves with complex morphology (e.g., needles), the External Method presents limitations associated with gaps between the leaves, and the Internal Method presents uncertainties related to the estimation of total leaf area. We introduce a modified version of the Internal Method, which bypasses the effect of gaps and the need to estimate total leaf area, by painting the leaves black and measuring them before and after painting. We assess and compare the new method with the External Method using a broadleaf and two conifer species. Both methods yielded similar leaf absorption estimates for the broadleaf, but absorption estimates were higher with the External Method for the conifer species. Factors explaining the differences between methods, their trade-offs and their advantages and limitations are also discussed. We suggest that the new method can be used to estimate leaf absorption in any type of leaf independently of its morphology, and be used to study further the impact of gap fraction in the External Method.
Original languageEnglish
JournalTree Physiology
Volume36
Issue number3
Pages (from-to)368-379
Number of pages12
ISSN0829-318X
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 4112 Forestry
  • conifer
  • integrating sphere
  • reflectance
  • transmittance
  • REVISED MEASUREMENT METHODOLOGY
  • SPECTRAL OPTICAL-PROPERTIES
  • RADIATIVE-TRANSFER MODEL
  • CONIFER NEEDLES
  • INTEGRATING SPHERE
  • REFLECTANCE
  • LEAF
  • TRANSMITTANCE
  • EFFICIENCY
  • PROSPECT

Cite this

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title = "A comparison of methods to estimate photosynthetic light absorption in leaves with contrasting morphology",
abstract = "Accurate temporal and spatial measurements of leaf optical traits (i.e., absorption, reflectance and transmittance) are paramount to photosynthetic studies. These optical traits are also needed to couple radiative transfer and physiological models to facilitate the interpretation of optical data. However, estimating leaf optical traits in leaves with complex morphologies remains a challenge. Leaf optical traits can be measured using integrating spheres, either by placing the leaf sample in one of the measuring ports (External Method) or by placing the sample inside the sphere (Internal Method). However, in leaves with complex morphology (e.g., needles), the External Method presents limitations associated with gaps between the leaves, and the Internal Method presents uncertainties related to the estimation of total leaf area. We introduce a modified version of the Internal Method, which bypasses the effect of gaps and the need to estimate total leaf area, by painting the leaves black and measuring them before and after painting. We assess and compare the new method with the External Method using a broadleaf and two conifer species. Both methods yielded similar leaf absorption estimates for the broadleaf, but absorption estimates were higher with the External Method for the conifer species. Factors explaining the differences between methods, their trade-offs and their advantages and limitations are also discussed. We suggest that the new method can be used to estimate leaf absorption in any type of leaf independently of its morphology, and be used to study further the impact of gap fraction in the External Method.",
keywords = "4112 Forestry, conifer , integrating sphere , reflectance , transmittance , REVISED MEASUREMENT METHODOLOGY , SPECTRAL OPTICAL-PROPERTIES, RADIATIVE-TRANSFER MODEL , CONIFER NEEDLES , INTEGRATING SPHERE , REFLECTANCE , LEAF , TRANSMITTANCE , EFFICIENCY , PROSPECT",
author = "Benat Olascoaga and Alasdair MacArthur and Jon Atherton and Albert Porcar-Castell",
year = "2016",
doi = "10.1093/treephys/tpv133",
language = "English",
volume = "36",
pages = "368--379",
journal = "Tree Physiology",
issn = "0829-318X",
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A comparison of methods to estimate photosynthetic light absorption in leaves with contrasting morphology. / Olascoaga, Benat; MacArthur, Alasdair; Atherton, Jon; Porcar-Castell, Albert.

In: Tree Physiology, Vol. 36, No. 3, 2016, p. 368-379.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A comparison of methods to estimate photosynthetic light absorption in leaves with contrasting morphology

AU - Olascoaga, Benat

AU - MacArthur, Alasdair

AU - Atherton, Jon

AU - Porcar-Castell, Albert

PY - 2016

Y1 - 2016

N2 - Accurate temporal and spatial measurements of leaf optical traits (i.e., absorption, reflectance and transmittance) are paramount to photosynthetic studies. These optical traits are also needed to couple radiative transfer and physiological models to facilitate the interpretation of optical data. However, estimating leaf optical traits in leaves with complex morphologies remains a challenge. Leaf optical traits can be measured using integrating spheres, either by placing the leaf sample in one of the measuring ports (External Method) or by placing the sample inside the sphere (Internal Method). However, in leaves with complex morphology (e.g., needles), the External Method presents limitations associated with gaps between the leaves, and the Internal Method presents uncertainties related to the estimation of total leaf area. We introduce a modified version of the Internal Method, which bypasses the effect of gaps and the need to estimate total leaf area, by painting the leaves black and measuring them before and after painting. We assess and compare the new method with the External Method using a broadleaf and two conifer species. Both methods yielded similar leaf absorption estimates for the broadleaf, but absorption estimates were higher with the External Method for the conifer species. Factors explaining the differences between methods, their trade-offs and their advantages and limitations are also discussed. We suggest that the new method can be used to estimate leaf absorption in any type of leaf independently of its morphology, and be used to study further the impact of gap fraction in the External Method.

AB - Accurate temporal and spatial measurements of leaf optical traits (i.e., absorption, reflectance and transmittance) are paramount to photosynthetic studies. These optical traits are also needed to couple radiative transfer and physiological models to facilitate the interpretation of optical data. However, estimating leaf optical traits in leaves with complex morphologies remains a challenge. Leaf optical traits can be measured using integrating spheres, either by placing the leaf sample in one of the measuring ports (External Method) or by placing the sample inside the sphere (Internal Method). However, in leaves with complex morphology (e.g., needles), the External Method presents limitations associated with gaps between the leaves, and the Internal Method presents uncertainties related to the estimation of total leaf area. We introduce a modified version of the Internal Method, which bypasses the effect of gaps and the need to estimate total leaf area, by painting the leaves black and measuring them before and after painting. We assess and compare the new method with the External Method using a broadleaf and two conifer species. Both methods yielded similar leaf absorption estimates for the broadleaf, but absorption estimates were higher with the External Method for the conifer species. Factors explaining the differences between methods, their trade-offs and their advantages and limitations are also discussed. We suggest that the new method can be used to estimate leaf absorption in any type of leaf independently of its morphology, and be used to study further the impact of gap fraction in the External Method.

KW - 4112 Forestry

KW - conifer

KW - integrating sphere

KW - reflectance

KW - transmittance

KW - REVISED MEASUREMENT METHODOLOGY

KW - SPECTRAL OPTICAL-PROPERTIES

KW - RADIATIVE-TRANSFER MODEL

KW - CONIFER NEEDLES

KW - INTEGRATING SPHERE

KW - REFLECTANCE

KW - LEAF

KW - TRANSMITTANCE

KW - EFFICIENCY

KW - PROSPECT

U2 - 10.1093/treephys/tpv133

DO - 10.1093/treephys/tpv133

M3 - Article

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SP - 368

EP - 379

JO - Tree Physiology

JF - Tree Physiology

SN - 0829-318X

IS - 3

ER -