A doubled haploid rye linkage map with a QTL affecting α-amylase activity

Teija Tenhola-Roininen, Ruslan Kalendar, Alan Schulman, Pirjo Tanhuanpää

Research output: Contribution to journalArticleScientificpeer-review

Abstract

A rye doubled haploid (DH) mapping population (Amilo × Voima) segregating for pre-harvest sprouting (PHS) was generated through anther culture of F 1 plants. A linkage map was constructed using DHs, to our knowledge, for the first time in rye. The map was composed of 289 loci: amplified fragment length polymorphism (AFLP), microsatellite, random amplified polymorphic DNA (RAPD), retrotransposon-microsatellite amplified polymorphism (REMAP), inter-retrotransposon amplified polymorphism (IRAP), and sequence-related amplified polymorphism (SRAP) markers, and extended altogether 732 cM (one locus in every 2.5 cM). All of the seven rye chromosomes and four unplaced groups were formed. Distorted segregation of markers (P≤0.05) was detected on all chromosomes. One major quantitative trait locus (QTL) affecting α-amylase activity was found, which explained 16.1% of phenotypic variation. The QTL was localized on the long arm of chromosome 5R. Microsatellites SCM74, RMS1115, and SCM77, nearest to the QTL, can be used for marker-assisted selection as a part of a rye breeding program to decrease sprouting damage.
Original languageEnglish
JournalJournal of Applied Genetics
Volume52
Issue number3
Pages (from-to)299-304
Number of pages6
ISSN1234-1983
DOIs
Publication statusPublished - 2011
MoE publication typeA1 Journal article-refereed

Fields of Science

  • Microsatellite
  • Pre-harvest sprouting
  • Retrotransposon
  • Secale cereale L.
  • Segregation distortion
  • Sequence-related amplified polymorphism
  • SECALE-CEREALE L.
  • SPROUTING RESISTANCE
  • MICROSATELLITE
  • BARLEY
  • WHEAT
  • DNA
  • IDENTIFICATION
  • MARKERS
  • REMAP
  • GRAIN
  • 1182 Biochemistry, cell and molecular biology

Cite this

Tenhola-Roininen, Teija ; Kalendar, Ruslan ; Schulman, Alan ; Tanhuanpää, Pirjo. / A doubled haploid rye linkage map with a QTL affecting α-amylase activity. In: Journal of Applied Genetics. 2011 ; Vol. 52, No. 3. pp. 299-304.
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abstract = "A rye doubled haploid (DH) mapping population (Amilo × Voima) segregating for pre-harvest sprouting (PHS) was generated through anther culture of F 1 plants. A linkage map was constructed using DHs, to our knowledge, for the first time in rye. The map was composed of 289 loci: amplified fragment length polymorphism (AFLP), microsatellite, random amplified polymorphic DNA (RAPD), retrotransposon-microsatellite amplified polymorphism (REMAP), inter-retrotransposon amplified polymorphism (IRAP), and sequence-related amplified polymorphism (SRAP) markers, and extended altogether 732 cM (one locus in every 2.5 cM). All of the seven rye chromosomes and four unplaced groups were formed. Distorted segregation of markers (P≤0.05) was detected on all chromosomes. One major quantitative trait locus (QTL) affecting α-amylase activity was found, which explained 16.1{\%} of phenotypic variation. The QTL was localized on the long arm of chromosome 5R. Microsatellites SCM74, RMS1115, and SCM77, nearest to the QTL, can be used for marker-assisted selection as a part of a rye breeding program to decrease sprouting damage.",
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A doubled haploid rye linkage map with a QTL affecting α-amylase activity. / Tenhola-Roininen, Teija; Kalendar, Ruslan; Schulman, Alan; Tanhuanpää, Pirjo.

In: Journal of Applied Genetics, Vol. 52, No. 3, 2011, p. 299-304.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - A doubled haploid rye linkage map with a QTL affecting α-amylase activity

AU - Tenhola-Roininen, Teija

AU - Kalendar, Ruslan

AU - Schulman, Alan

AU - Tanhuanpää, Pirjo

PY - 2011

Y1 - 2011

N2 - A rye doubled haploid (DH) mapping population (Amilo × Voima) segregating for pre-harvest sprouting (PHS) was generated through anther culture of F 1 plants. A linkage map was constructed using DHs, to our knowledge, for the first time in rye. The map was composed of 289 loci: amplified fragment length polymorphism (AFLP), microsatellite, random amplified polymorphic DNA (RAPD), retrotransposon-microsatellite amplified polymorphism (REMAP), inter-retrotransposon amplified polymorphism (IRAP), and sequence-related amplified polymorphism (SRAP) markers, and extended altogether 732 cM (one locus in every 2.5 cM). All of the seven rye chromosomes and four unplaced groups were formed. Distorted segregation of markers (P≤0.05) was detected on all chromosomes. One major quantitative trait locus (QTL) affecting α-amylase activity was found, which explained 16.1% of phenotypic variation. The QTL was localized on the long arm of chromosome 5R. Microsatellites SCM74, RMS1115, and SCM77, nearest to the QTL, can be used for marker-assisted selection as a part of a rye breeding program to decrease sprouting damage.

AB - A rye doubled haploid (DH) mapping population (Amilo × Voima) segregating for pre-harvest sprouting (PHS) was generated through anther culture of F 1 plants. A linkage map was constructed using DHs, to our knowledge, for the first time in rye. The map was composed of 289 loci: amplified fragment length polymorphism (AFLP), microsatellite, random amplified polymorphic DNA (RAPD), retrotransposon-microsatellite amplified polymorphism (REMAP), inter-retrotransposon amplified polymorphism (IRAP), and sequence-related amplified polymorphism (SRAP) markers, and extended altogether 732 cM (one locus in every 2.5 cM). All of the seven rye chromosomes and four unplaced groups were formed. Distorted segregation of markers (P≤0.05) was detected on all chromosomes. One major quantitative trait locus (QTL) affecting α-amylase activity was found, which explained 16.1% of phenotypic variation. The QTL was localized on the long arm of chromosome 5R. Microsatellites SCM74, RMS1115, and SCM77, nearest to the QTL, can be used for marker-assisted selection as a part of a rye breeding program to decrease sprouting damage.

KW - Microsatellite

KW - Pre-harvest sprouting

KW - Retrotransposon

KW - Secale cereale L.

KW - Segregation distortion

KW - Sequence-related amplified polymorphism

KW - SECALE-CEREALE L.

KW - SPROUTING RESISTANCE

KW - MICROSATELLITE

KW - BARLEY

KW - WHEAT

KW - DNA

KW - IDENTIFICATION

KW - MARKERS

KW - REMAP

KW - GRAIN

KW - 1182 Biochemistry, cell and molecular biology

U2 - 10.1007/s13353-011-0029-1

DO - 10.1007/s13353-011-0029-1

M3 - Article

VL - 52

SP - 299

EP - 304

JO - Journal of Applied Genetics

JF - Journal of Applied Genetics

SN - 1234-1983

IS - 3

ER -