A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family

Filip Mollerup, Ville Aumala, Kirsti Maria Parikka, Yan Mathieu, Harry Brumer, Tiina Maija Tenkanen, Emma Master

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Copper radical alcohol oxidases belonging to auxiliary activity family 5, subfamily 2 (AA5_2) catalyze the oxidation of galactose and galactosides, as well as aliphatic alcohols. Despite their broad applied potential, so far very few AA5_2 members have been biochemically characterized. We report the recombinant production and biochemical characterization of an AA5_2 oxidase from Penicillium rubens Wisconsin 54-1255 (PruAA5_2A), which groups within an unmapped clade phylogenetically distant from those comprising AA5_2 members characterized to date. PruAA5_2 preferentially oxidized raffinose over galactose; however, its catalytic efficiency was 6.5 times higher on glycolaldehyde dimer compared to raffinose. Deep sequence analysis of characterized AA5_2 members highlighted amino acid pairs correlated to substrate range and conserved within the family. Moreover, PruAA5_2 activity spans substrate preferences previously reported for AA5 subfamily 1 and 2 members, identifying possible functional overlap across the AA5 family.

Original languageEnglish
Article number0216546
JournalPLoS One
Volume14
Issue number5
Pages (from-to)1-19
Number of pages19
ISSN1932-6203
DOIs
Publication statusPublished - 15 May 2019
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 414 Agricultural biotechnology
  • 1183 Plant biology, microbiology, virology
  • GALACTOSE-OXIDASE
  • GLYOXAL OXIDASE
  • BINDING MODULE
  • ENZYME
  • EXPRESSION
  • MECHANISM
  • OXIDATION
  • AEROGELS
  • GENE

Cite this

Mollerup, Filip ; Aumala, Ville ; Parikka, Kirsti Maria ; Mathieu, Yan ; Brumer, Harry ; Tenkanen, Tiina Maija ; Master, Emma. / A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family. In: PLoS One. 2019 ; Vol. 14, No. 5. pp. 1-19.
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title = "A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family",
abstract = "Copper radical alcohol oxidases belonging to auxiliary activity family 5, subfamily 2 (AA5_2) catalyze the oxidation of galactose and galactosides, as well as aliphatic alcohols. Despite their broad applied potential, so far very few AA5_2 members have been biochemically characterized. We report the recombinant production and biochemical characterization of an AA5_2 oxidase from Penicillium rubens Wisconsin 54-1255 (PruAA5_2A), which groups within an unmapped clade phylogenetically distant from those comprising AA5_2 members characterized to date. PruAA5_2 preferentially oxidized raffinose over galactose; however, its catalytic efficiency was 6.5 times higher on glycolaldehyde dimer compared to raffinose. Deep sequence analysis of characterized AA5_2 members highlighted amino acid pairs correlated to substrate range and conserved within the family. Moreover, PruAA5_2 activity spans substrate preferences previously reported for AA5 subfamily 1 and 2 members, identifying possible functional overlap across the AA5 family.",
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author = "Filip Mollerup and Ville Aumala and Parikka, {Kirsti Maria} and Yan Mathieu and Harry Brumer and Tenkanen, {Tiina Maija} and Emma Master",
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A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family. / Mollerup, Filip; Aumala, Ville; Parikka, Kirsti Maria; Mathieu, Yan; Brumer, Harry; Tenkanen, Tiina Maija; Master, Emma.

In: PLoS One, Vol. 14, No. 5, 0216546, 15.05.2019, p. 1-19.

Research output: Contribution to journalArticleScientificpeer-review

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T1 - A family AA5_2 carbohydrate oxidase from Penicillium rubens displays functional overlap across the AA5 family

AU - Mollerup, Filip

AU - Aumala, Ville

AU - Parikka, Kirsti Maria

AU - Mathieu, Yan

AU - Brumer, Harry

AU - Tenkanen, Tiina Maija

AU - Master, Emma

PY - 2019/5/15

Y1 - 2019/5/15

N2 - Copper radical alcohol oxidases belonging to auxiliary activity family 5, subfamily 2 (AA5_2) catalyze the oxidation of galactose and galactosides, as well as aliphatic alcohols. Despite their broad applied potential, so far very few AA5_2 members have been biochemically characterized. We report the recombinant production and biochemical characterization of an AA5_2 oxidase from Penicillium rubens Wisconsin 54-1255 (PruAA5_2A), which groups within an unmapped clade phylogenetically distant from those comprising AA5_2 members characterized to date. PruAA5_2 preferentially oxidized raffinose over galactose; however, its catalytic efficiency was 6.5 times higher on glycolaldehyde dimer compared to raffinose. Deep sequence analysis of characterized AA5_2 members highlighted amino acid pairs correlated to substrate range and conserved within the family. Moreover, PruAA5_2 activity spans substrate preferences previously reported for AA5 subfamily 1 and 2 members, identifying possible functional overlap across the AA5 family.

AB - Copper radical alcohol oxidases belonging to auxiliary activity family 5, subfamily 2 (AA5_2) catalyze the oxidation of galactose and galactosides, as well as aliphatic alcohols. Despite their broad applied potential, so far very few AA5_2 members have been biochemically characterized. We report the recombinant production and biochemical characterization of an AA5_2 oxidase from Penicillium rubens Wisconsin 54-1255 (PruAA5_2A), which groups within an unmapped clade phylogenetically distant from those comprising AA5_2 members characterized to date. PruAA5_2 preferentially oxidized raffinose over galactose; however, its catalytic efficiency was 6.5 times higher on glycolaldehyde dimer compared to raffinose. Deep sequence analysis of characterized AA5_2 members highlighted amino acid pairs correlated to substrate range and conserved within the family. Moreover, PruAA5_2 activity spans substrate preferences previously reported for AA5 subfamily 1 and 2 members, identifying possible functional overlap across the AA5 family.

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KW - 1183 Plant biology, microbiology, virology

KW - GALACTOSE-OXIDASE

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KW - OXIDATION

KW - AEROGELS

KW - GENE

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