A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana

RW  Nowell; B Elsworth; Vicencio Oostra; Bas J. Zwaan; Christopher West Wheat; Marjo Anna Kaarina Saastamoinen; Ilik Saccheri; AE Van't Hof; BR Wasik; H Connahs; S Kumar; RJ Challis; L Aslam; Antonia Monteiro; Paul M. Brakefield; M Blaxter

doi:10.1093/gigascience/gix035

A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana

RW Nowell, B Elsworth, Vicencio Oostra, Bas J. Zwaan, Christopher West Wheat, Marjo Anna Kaarina Saastamoinen, Ilik Saccheri, AE Van't Hof, BR Wasik, H Connahs, S Kumar, RJ Challis, L Aslam, Antonia Monteiro, Paul M. Brakefield, M Blaxter

Life-history Evolution Research Group

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

The mycalesine butterfly Bicyclus anynana, the “Squinting bush brown,” is a model organism in the study of lepidopteran ecology, development, and evolution. Here, we present a draft genome sequence for B. anynana to serve as a genomics resource for current and future studies of this important model species. Seven libraries with insert sizes ranging from 350 bp to 20 kb were constructed using DNA from an inbred female and sequenced using both Illumina and PacBio technology; 128 Gb of raw Illumina data was filtered to 124 Gb and assembled to a final size of 475 Mb (∼×260 assembly coverage). Contigs were scaffolded using mate-pair, transcriptome, and PacBio data into 10 800 sequences with an N50 of 638 kb (longest scaffold 5 Mb). The genome is comprised of 26% repetitive elements and encodes a total of 22 642 predicted protein-coding genes. Recovery of a BUSCO set of core metazoan genes was almost complete (98%). Overall, these metrics compare well with other recently published lepidopteran genomes. We report a high-quality draft genome sequence for
Bicyclus anynana. The genome assembly and annotated gene models are available at LepBase (http://ensembl.lepbase.org/index.html).

Original language	English
Journal	GigaScience
Volume	6
Pages (from-to)	1-7
Number of pages	7
ISSN	2047-217X
DOIs	https://doi.org/10.1093/gigascience/gix035
Publication status	Published - 2017
MoE publication type	A1 Journal article-refereed

Fields of Science

1181 Ecology, evolutionary biology

Access to Document

10.1093/gigascience/gix035Licence: CC BY

Nowell et al. 2017_GIGA SciFinal published version, 948 KBLicence: CC BY

Cite this

Nowell, RW., Elsworth, B., Oostra, V., Zwaan, B. J., Wheat, C. W., Saastamoinen, M. A. K., Saccheri, I., Van't Hof, AE., Wasik, BR., Connahs, H., Kumar, S., Challis, RJ., Aslam, L., Monteiro, A., Brakefield, P. M., & Blaxter, M. (2017). A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana. GigaScience, 6, 1-7. https://doi.org/10.1093/gigascience/gix035

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abstract = "The mycalesine butterfly Bicyclus anynana, the “Squinting bush brown,” is a model organism in the study of lepidopteran ecology, development, and evolution. Here, we present a draft genome sequence for B. anynana to serve as a genomics resource for current and future studies of this important model species. Seven libraries with insert sizes ranging from 350 bp to 20 kb were constructed using DNA from an inbred female and sequenced using both Illumina and PacBio technology; 128 Gb of raw Illumina data was filtered to 124 Gb and assembled to a final size of 475 Mb (∼×260 assembly coverage). Contigs were scaffolded using mate-pair, transcriptome, and PacBio data into 10 800 sequences with an N50 of 638 kb (longest scaffold 5 Mb). The genome is comprised of 26% repetitive elements and encodes a total of 22 642 predicted protein-coding genes. Recovery of a BUSCO set of core metazoan genes was almost complete (98%). Overall, these metrics compare well with other recently published lepidopteran genomes. We report a high-quality draft genome sequence forBicyclus anynana. The genome assembly and annotated gene models are available at LepBase (http://ensembl.lepbase.org/index.html).",

keywords = "1181 Ecology, evolutionary biology",

author = "RW Nowell and B Elsworth and Vicencio Oostra and Zwaan, {Bas J.} and Wheat, {Christopher West} and Saastamoinen, {Marjo Anna Kaarina} and Ilik Saccheri and {Van't Hof}, AE and BR Wasik and H Connahs and S Kumar and RJ Challis and L Aslam and Antonia Monteiro and Brakefield, {Paul M.} and M Blaxter",

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AU - Nowell, RW

AU - Elsworth, B

AU - Oostra, Vicencio

AU - Zwaan, Bas J.

AU - Wheat, Christopher West

AU - Saastamoinen, Marjo Anna Kaarina

AU - Saccheri, Ilik

AU - Van't Hof, AE

AU - Wasik, BR

AU - Connahs, H

AU - Kumar, S

AU - Challis, RJ

AU - Aslam, L

AU - Monteiro, Antonia

AU - Brakefield, Paul M.

AU - Blaxter, M

PY - 2017

Y1 - 2017

N2 - The mycalesine butterfly Bicyclus anynana, the “Squinting bush brown,” is a model organism in the study of lepidopteran ecology, development, and evolution. Here, we present a draft genome sequence for B. anynana to serve as a genomics resource for current and future studies of this important model species. Seven libraries with insert sizes ranging from 350 bp to 20 kb were constructed using DNA from an inbred female and sequenced using both Illumina and PacBio technology; 128 Gb of raw Illumina data was filtered to 124 Gb and assembled to a final size of 475 Mb (∼×260 assembly coverage). Contigs were scaffolded using mate-pair, transcriptome, and PacBio data into 10 800 sequences with an N50 of 638 kb (longest scaffold 5 Mb). The genome is comprised of 26% repetitive elements and encodes a total of 22 642 predicted protein-coding genes. Recovery of a BUSCO set of core metazoan genes was almost complete (98%). Overall, these metrics compare well with other recently published lepidopteran genomes. We report a high-quality draft genome sequence forBicyclus anynana. The genome assembly and annotated gene models are available at LepBase (http://ensembl.lepbase.org/index.html).

AB - The mycalesine butterfly Bicyclus anynana, the “Squinting bush brown,” is a model organism in the study of lepidopteran ecology, development, and evolution. Here, we present a draft genome sequence for B. anynana to serve as a genomics resource for current and future studies of this important model species. Seven libraries with insert sizes ranging from 350 bp to 20 kb were constructed using DNA from an inbred female and sequenced using both Illumina and PacBio technology; 128 Gb of raw Illumina data was filtered to 124 Gb and assembled to a final size of 475 Mb (∼×260 assembly coverage). Contigs were scaffolded using mate-pair, transcriptome, and PacBio data into 10 800 sequences with an N50 of 638 kb (longest scaffold 5 Mb). The genome is comprised of 26% repetitive elements and encodes a total of 22 642 predicted protein-coding genes. Recovery of a BUSCO set of core metazoan genes was almost complete (98%). Overall, these metrics compare well with other recently published lepidopteran genomes. We report a high-quality draft genome sequence forBicyclus anynana. The genome assembly and annotated gene models are available at LepBase (http://ensembl.lepbase.org/index.html).

KW - 1181 Ecology, evolutionary biology

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