A Key Role for Apoplastic H2O2 in Norway Spruce Phenolic Metabolism

Teresa Pauliina Laitinen, Kris Morreel, Nicolas Delhomme, Adrien Guy Bernard Gauthier, Bastian Schiffthaler, Kaloian Iliev Nickolov, Gunter Brader, Kean-Jin Lim, Teemu Heikki Teeri, Nathaniel R. Street, Wout Boerjan, Anna Kristiina Kärkönen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Apoplastic events such as monolignol oxidation and lignin polymerization are difficult to study in intact trees. To investigate the role of apoplastic H2O2 in gymnosperm phenolic metabolism, an extracellular lignin-forming cell culture of Norway spruce (Picea abies) was used as a research model. Scavenging of apoplastic H2O2 by KI repressed lignin formation, in line with peroxidases activating monolignols for lignin polymerization. Time course analyses coupled to candidate substrate product pair network propagation revealed differential accumulation of low-molecular-weight phenolics, including (glycosylated) oligolignols, (glycosylated) flavonoids and proanthocyanidins, in lignin-forming and H2O2-scavenging cultures, and supported that monolignols are oxidatively coupled not only in the cell wall, but also in the cytoplasm where they are coupled to other monolignols and proanthocyanidins. Dilignol glycoconjugates with reduced structures were found in the culture medium, suggesting that cells are able to transport glycosylated dilignols to the apoplast. Transcriptomic analyses revealed that scavenging of apoplastic H2O2 resulted in remodulation of the transcriptome, with reduced carbon flux into the shikimate pathway propagating down to monolignol biosynthesis. Aggregated co-expression network analysis identified candidate enzymes and transcription factors for monolignol oxidation and apoplastic H2O2 production in addition to potential H2O2 receptors. The results presented indicate that the redox state of the apoplast has a profound influence on cellular metabolism.
Original languageEnglish
JournalPlant Physiology
Volume174
Pages (from-to)1449-1475
Number of pages7
ISSN0032-0889
DOIs
Publication statusPublished - 18 May 2017
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1183 Plant biology, microbiology, virology

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