A layered solid-state culture system for investigating the fungal growth and decay behaviour on the cellulosic substrate

Mycelium-based materials have attracted growing interest, facilitating their development for various new applications. Finding for suitable fungal species and strains enables further technical breakthroughs in their development and quality control. Here, we developed a model solid-state culture system to screen fungal strains efficiently for mycelium-based materials production. A piece of silicone-coated paper set on the general agar plate allows for isolation of the mycelial mat from the substrate. The mycelial growth and density can be evaluated by weighing the mycelial mat. We used the paper substrate after fungal incubation to investigate the relationship between substrate degradation and the contact time with hyphae. It yielded further insights into the fungal decay. Ten basidiomycetes were assessed for their fungal growth and degradation behaviour of the substrate using this method. Pleurotus floridanus FBCC375 showed a dense and elastic mycelial mat and mild degradation of the substrate. A unique decay behaviour was found in Hypsizygus ulmarius FBCC573 and Trametes versicolor FBCC564. They indicated a positional imbalance in the decay activity within the colony. This simple method is helpful for screening fungal strains and facilitates the further development of mycelium-based materials.