Abstract
DNA typing is essential for identifying crime scene evidence and missing and unknown persons. Molecular tags historically have been incorporated into DNA typing reactions to improve result interpretation. Molecular tags like barcodes and unique identifiers are integral to MPS, aiding in sample tracking and error detection. However, these tags do not fully leverage sequence variation to enhance quality control. To address this need, molecular etches, which are synthetic oligonucleotides that serve as an internal molecular information management system, are introduced. Molecular etches encode detailed sample information improving sample workflow history, tracking, contamination detection, and authenticity verification. Validation studies demonstrate the robustness of molecular etches in genomic sequencing, making them a valuable quality tool for forensic DNA analysis.
Original language | English |
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Article number | 103179 |
Journal | Forensic Science International: Genetics |
Volume | 75 |
Number of pages | 7 |
ISSN | 1872-4973 |
DOIs | |
Publication status | Published - Feb 2025 |
MoE publication type | A1 Journal article-refereed |
Bibliographical note
Publisher Copyright:© 2024 The Authors
Fields of Science
- Internal Molecular Information System
- Massively Parallel Sequencing
- Molecular Etches
- Molecular Tags
- Validation Studies
- 319 Forensic science and other medical sciences