A simple phenol-free isolation method for high-quality RNA from bilberry

Research output: Contribution to journalArticleScientificpeer-review

Abstract

High-quality RNA is required for accurate gene expression and transcriptome analysis. The current methods of RNA extraction from berry fruits are either time-consuming or expensive. To simplify the conventional phenol chloroform based RNA extraction method, we modified the protocol with less steps as well as the removal of the use of phenol. In this protocol, the extraction buffer is composed of hexadecyltrimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP), and Dithiothreitol (DTT). The method facilitates efficient removal of polysaccharides and phenolic compounds from both fruit pulp and fruit peel. Additionally, the protocol is phenol free and less toxic than traditional phenol-containing method. High-quality RNA, with RNA Integrity Number value > 8, isolated by this method is applicable for RNA sequencing and qPCR. Only 3-4 working hours are required for one batch of RNA isolation.

Our method replaces the use of phenol-chloroform with chloroform, making the extraction less toxic.

The bilberry friut RNA is of high-quality and purity with less time input. (C) 2021 The Authors. Published by Elsevier B.V.

Original languageEnglish
Article number101481
JournalMethodsX
Volume8
Number of pages5
ISSN2215-0161
DOIs
Publication statusPublished - 9 Aug 2021
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 11831 Plant biology
  • RNA isolation
  • CTAB
  • Fruit pulp
  • Fruit peel
  • Bilberry
  • RIN

Cite this