Abstract
| Original language | English |
|---|---|
| Journal | Journal of General Virology |
| Volume | 87 |
| Issue number | 6 |
| Pages (from-to) | 1395-1407 |
| Number of pages | 13 |
| ISSN | 0022-1317 |
| DOIs | |
| Publication status | Published - 2016 |
| MoE publication type | A1 Journal article-refereed |
Fields of Science
- 1183 Plant biology, microbiology, virology
Cite this
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Ability of minus strands and modified plus strands to act as templates in Semliki Forest virus RNA replication. / Hellström, Kirsi Johanna; Kallio, Katri Anneli; Meriläinen, Hanna-Mari; Jokitalo, Eija Sofia; Ahola, Tero Tapani.
In: Journal of General Virology, Vol. 87, No. 6, 2016, p. 1395-1407.Research output: Contribution to journal › Article › Scientific › peer-review
TY - JOUR
T1 - Ability of minus strands and modified plus strands to act as templates in Semliki Forest virus RNA replication.
AU - Hellström, Kirsi Johanna
AU - Kallio, Katri Anneli
AU - Meriläinen, Hanna-Mari
AU - Jokitalo, Eija Sofia
AU - Ahola, Tero Tapani
PY - 2016
Y1 - 2016
N2 - During virus multiplication, the viral genome is recognized and recruited for replication based on specific cis-acting elements. Here we have dissected the important cis-acting sequence elements in Semliki Forest virus RNA, by using a trans-replication system. Since the viral replicase is expressed from a separate plasmid, the template RNA can be freely modified in this system. We show that the cis-acting element at the beginning of the non-structural protein 1 (nsP1) coding region together with the end of the 3′ untranslated region are the minimal requirements for minus strand synthesis. To achieve a high level of replication, the native 5′ untranslated region was also needed. The virus-induced membranous replication compartments or spherules were only detected when a replication-competent template was present with an active replicase and minus strands were produced. No translation could be detected from the minus strands, suggesting that they are segregated from the cytoplasm. Minus strands could not directly be recruited to initiate the replication process. Thus, there is only one defined pathway for replication, starting with plus strand recognition followed by concomitant spherule formation and minus strand synthesis.
AB - During virus multiplication, the viral genome is recognized and recruited for replication based on specific cis-acting elements. Here we have dissected the important cis-acting sequence elements in Semliki Forest virus RNA, by using a trans-replication system. Since the viral replicase is expressed from a separate plasmid, the template RNA can be freely modified in this system. We show that the cis-acting element at the beginning of the non-structural protein 1 (nsP1) coding region together with the end of the 3′ untranslated region are the minimal requirements for minus strand synthesis. To achieve a high level of replication, the native 5′ untranslated region was also needed. The virus-induced membranous replication compartments or spherules were only detected when a replication-competent template was present with an active replicase and minus strands were produced. No translation could be detected from the minus strands, suggesting that they are segregated from the cytoplasm. Minus strands could not directly be recruited to initiate the replication process. Thus, there is only one defined pathway for replication, starting with plus strand recognition followed by concomitant spherule formation and minus strand synthesis.
KW - 1183 Plant biology, microbiology, virology
U2 - 10.1099/jgv.0.000448
DO - 10.1099/jgv.0.000448
M3 - Article
VL - 87
SP - 1395
EP - 1407
JO - Journal of General Virology
JF - Journal of General Virology
SN - 0022-1317
IS - 6
ER -