Acidipropionibacterium virtanenii sp. nov., isolated from malted barley

Paulina Deptula, Olli-Pekka Aukusti Smolander, Pia Kati Sofia Laine, Richard J. Roberts, Johanna Minnamari Edelmann, Petri Peltola, Vieno Irene Piironen, Lars Göran Paulin, Erna Storgårds, Kirsi Savijoki, Arja Laitila, Petri Olli Viljami Auvinen, Pekka Kristian Varmanen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 %, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278(T) to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278(T) is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol%. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37 degrees C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacterium virtanenii sp. nov. The type strain is JS278(T) (=VTT E-113202(T)=DSM 106790(T)).

Original languageEnglish
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue number10
Pages (from-to)3175-3183
Number of pages9
ISSN1466-5026
DOIs
Publication statusPublished - Oct 2018
MoE publication typeA1 Journal article-refereed

Fields of Science

  • A. acidipropionici
  • A. jensenii
  • Acidipropionibacterium virtanenii
  • BACTERIOCIN
  • CLASSIFICATION
  • FREUDENREICHII
  • GENES
  • GENOMES
  • JENSENII
  • NEIGHBOR-JOINING METHOD
  • PROPIONIBACTERIUM-THOENII
  • RECLASSIFICATION
  • TOOL
  • defence mechanisms
  • malted barley
  • pseudovitamin B12
  • 1183 Plant biology, microbiology, virology

Cite this

@article{2ea10f1550bd4ba1b5be3b5ff89d8648,
title = "Acidipropionibacterium virtanenii sp. nov., isolated from malted barley",
abstract = "A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 {\%}, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278(T) to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278(T) is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol{\%}. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37 degrees C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacterium virtanenii sp. nov. The type strain is JS278(T) (=VTT E-113202(T)=DSM 106790(T)).",
keywords = "A. acidipropionici, A. jensenii, Acidipropionibacterium virtanenii, BACTERIOCIN, CLASSIFICATION, FREUDENREICHII, GENES, GENOMES, JENSENII, NEIGHBOR-JOINING METHOD, PROPIONIBACTERIUM-THOENII, RECLASSIFICATION, TOOL, defence mechanisms, malted barley, pseudovitamin B12, 1183 Plant biology, microbiology, virology",
author = "Paulina Deptula and Smolander, {Olli-Pekka Aukusti} and Laine, {Pia Kati Sofia} and Roberts, {Richard J.} and Edelmann, {Johanna Minnamari} and Petri Peltola and Piironen, {Vieno Irene} and Paulin, {Lars G{\"o}ran} and Erna Storg{\aa}rds and Kirsi Savijoki and Arja Laitila and Auvinen, {Petri Olli Viljami} and Varmanen, {Pekka Kristian}",
year = "2018",
month = "10",
doi = "10.1099/ijsem.0.002965",
language = "English",
volume = "68",
pages = "3175--3183",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "American Society for Microbiology",
number = "10",

}

Acidipropionibacterium virtanenii sp. nov., isolated from malted barley. / Deptula, Paulina; Smolander, Olli-Pekka Aukusti; Laine, Pia Kati Sofia; Roberts, Richard J.; Edelmann, Johanna Minnamari; Peltola, Petri; Piironen, Vieno Irene; Paulin, Lars Göran; Storgårds, Erna; Savijoki, Kirsi; Laitila, Arja; Auvinen, Petri Olli Viljami; Varmanen, Pekka Kristian.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 68, No. 10, 10.2018, p. 3175-3183.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Acidipropionibacterium virtanenii sp. nov., isolated from malted barley

AU - Deptula, Paulina

AU - Smolander, Olli-Pekka Aukusti

AU - Laine, Pia Kati Sofia

AU - Roberts, Richard J.

AU - Edelmann, Johanna Minnamari

AU - Peltola, Petri

AU - Piironen, Vieno Irene

AU - Paulin, Lars Göran

AU - Storgårds, Erna

AU - Savijoki, Kirsi

AU - Laitila, Arja

AU - Auvinen, Petri Olli Viljami

AU - Varmanen, Pekka Kristian

PY - 2018/10

Y1 - 2018/10

N2 - A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 %, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278(T) to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278(T) is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol%. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37 degrees C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacterium virtanenii sp. nov. The type strain is JS278(T) (=VTT E-113202(T)=DSM 106790(T)).

AB - A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 %, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278(T) to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278(T) is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol%. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37 degrees C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacterium virtanenii sp. nov. The type strain is JS278(T) (=VTT E-113202(T)=DSM 106790(T)).

KW - A. acidipropionici

KW - A. jensenii

KW - Acidipropionibacterium virtanenii

KW - BACTERIOCIN

KW - CLASSIFICATION

KW - FREUDENREICHII

KW - GENES

KW - GENOMES

KW - JENSENII

KW - NEIGHBOR-JOINING METHOD

KW - PROPIONIBACTERIUM-THOENII

KW - RECLASSIFICATION

KW - TOOL

KW - defence mechanisms

KW - malted barley

KW - pseudovitamin B12

KW - 1183 Plant biology, microbiology, virology

U2 - 10.1099/ijsem.0.002965

DO - 10.1099/ijsem.0.002965

M3 - Article

VL - 68

SP - 3175

EP - 3183

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 10

ER -