Advances in Biotechnology

Relation between the actinobacterial succession and microbial safety in aerobic waste treatment (composting).

S. Shivaveera Kumar, Nan Hui, Maija Kirsi, Agsar Dayanand, Petri Auvinen, Sirpa Laitinen, Merja Kontro

Research output: Chapter in Book/Report/Conference proceedingChapterProfessional

Abstract

Actinobacteria belong to the normal flora of soil; they degrade biological material in the aerobic waste treatment called composting. Although the actinobacterial succession during the composting may be possible to use as an indicator of microbiologically safe compost, their diversity and succession is not known well enough for this purpose. We studied the actinobacterial diversity and succession in composts ranging from 7 to 578 days using pyrosequencing. The template DNA was isolated from the composts and 16S rDNA was amplified with primers pB and StrepE, followed by pyrosequencing. The indicator bacteria Escherichia coli and Enterococcus spp. were cultivated and Yersinia spp., Campylobacter spp., Bacillus cereus, Salmonella spp. and Streptomyces spp. were quantified by real-time PCR. The results indicated that changes in actinobacteria, opposite to those in indicator and harmful bacteria, could be used as indicators for the absence pathogenic bacteria from compost. Still the order of magnitude of Streptomyces spp. in the compost was such high that their spores might be harmful for humans handling the compost material daily, necessitating their protection.
Original languageEnglish
Title of host publicationAdvances in Biotechnology : Relation between the actinobacterial succession and microbial safety in aerobic waste treatment (composting).
EditorsNeelu N. Nawani, Madhukar Khetmalas, P.N. Razdan, Ashok Pandey
Number of pages12
Place of PublicationNew Delhi
PublisherI.K. International Publishing House Pvt. Ltd.
Publication date2015
ISBN (Print)978-93-84588-09-0
Publication statusPublished - 2015
MoE publication typeD2 Article in professional manuals or guides or professional information systems or text book material

Fields of Science

  • 1172 Environmental sciences

Cite this

Kumar, S. S., Hui, N., Kirsi, M., Dayanand, A., Auvinen, P., Laitinen, S., & Kontro, M. (2015). Advances in Biotechnology: Relation between the actinobacterial succession and microbial safety in aerobic waste treatment (composting). In N. N. Nawani, M. Khetmalas, P. N. Razdan, & A. Pandey (Eds.), Advances in Biotechnology: Relation between the actinobacterial succession and microbial safety in aerobic waste treatment (composting). New Delhi: I.K. International Publishing House Pvt. Ltd..
Kumar, S. Shivaveera ; Hui, Nan ; Kirsi, Maija ; Dayanand, Agsar ; Auvinen, Petri ; Laitinen, Sirpa ; Kontro, Merja. / Advances in Biotechnology : Relation between the actinobacterial succession and microbial safety in aerobic waste treatment (composting). Advances in Biotechnology: Relation between the actinobacterial succession and microbial safety in aerobic waste treatment (composting).. editor / Neelu N. Nawani ; Madhukar Khetmalas ; P.N. Razdan ; Ashok Pandey. New Delhi : I.K. International Publishing House Pvt. Ltd., 2015.
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title = "Advances in Biotechnology: Relation between the actinobacterial succession and microbial safety in aerobic waste treatment (composting).",
abstract = "Actinobacteria belong to the normal flora of soil; they degrade biological material in the aerobic waste treatment called composting. Although the actinobacterial succession during the composting may be possible to use as an indicator of microbiologically safe compost, their diversity and succession is not known well enough for this purpose. We studied the actinobacterial diversity and succession in composts ranging from 7 to 578 days using pyrosequencing. The template DNA was isolated from the composts and 16S rDNA was amplified with primers pB and StrepE, followed by pyrosequencing. The indicator bacteria Escherichia coli and Enterococcus spp. were cultivated and Yersinia spp., Campylobacter spp., Bacillus cereus, Salmonella spp. and Streptomyces spp. were quantified by real-time PCR. The results indicated that changes in actinobacteria, opposite to those in indicator and harmful bacteria, could be used as indicators for the absence pathogenic bacteria from compost. Still the order of magnitude of Streptomyces spp. in the compost was such high that their spores might be harmful for humans handling the compost material daily, necessitating their protection.",
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Kumar, SS, Hui, N, Kirsi, M, Dayanand, A, Auvinen, P, Laitinen, S & Kontro, M 2015, Advances in Biotechnology: Relation between the actinobacterial succession and microbial safety in aerobic waste treatment (composting). in NN Nawani, M Khetmalas, PN Razdan & A Pandey (eds), Advances in Biotechnology: Relation between the actinobacterial succession and microbial safety in aerobic waste treatment (composting).. I.K. International Publishing House Pvt. Ltd., New Delhi.

Advances in Biotechnology : Relation between the actinobacterial succession and microbial safety in aerobic waste treatment (composting). / Kumar, S. Shivaveera; Hui, Nan; Kirsi, Maija; Dayanand, Agsar; Auvinen, Petri; Laitinen, Sirpa; Kontro, Merja.

Advances in Biotechnology: Relation between the actinobacterial succession and microbial safety in aerobic waste treatment (composting).. ed. / Neelu N. Nawani; Madhukar Khetmalas; P.N. Razdan; Ashok Pandey. New Delhi : I.K. International Publishing House Pvt. Ltd., 2015.

Research output: Chapter in Book/Report/Conference proceedingChapterProfessional

TY - CHAP

T1 - Advances in Biotechnology

T2 - Relation between the actinobacterial succession and microbial safety in aerobic waste treatment (composting).

AU - Kumar, S. Shivaveera

AU - Hui, Nan

AU - Kirsi, Maija

AU - Dayanand, Agsar

AU - Auvinen, Petri

AU - Laitinen, Sirpa

AU - Kontro, Merja

PY - 2015

Y1 - 2015

N2 - Actinobacteria belong to the normal flora of soil; they degrade biological material in the aerobic waste treatment called composting. Although the actinobacterial succession during the composting may be possible to use as an indicator of microbiologically safe compost, their diversity and succession is not known well enough for this purpose. We studied the actinobacterial diversity and succession in composts ranging from 7 to 578 days using pyrosequencing. The template DNA was isolated from the composts and 16S rDNA was amplified with primers pB and StrepE, followed by pyrosequencing. The indicator bacteria Escherichia coli and Enterococcus spp. were cultivated and Yersinia spp., Campylobacter spp., Bacillus cereus, Salmonella spp. and Streptomyces spp. were quantified by real-time PCR. The results indicated that changes in actinobacteria, opposite to those in indicator and harmful bacteria, could be used as indicators for the absence pathogenic bacteria from compost. Still the order of magnitude of Streptomyces spp. in the compost was such high that their spores might be harmful for humans handling the compost material daily, necessitating their protection.

AB - Actinobacteria belong to the normal flora of soil; they degrade biological material in the aerobic waste treatment called composting. Although the actinobacterial succession during the composting may be possible to use as an indicator of microbiologically safe compost, their diversity and succession is not known well enough for this purpose. We studied the actinobacterial diversity and succession in composts ranging from 7 to 578 days using pyrosequencing. The template DNA was isolated from the composts and 16S rDNA was amplified with primers pB and StrepE, followed by pyrosequencing. The indicator bacteria Escherichia coli and Enterococcus spp. were cultivated and Yersinia spp., Campylobacter spp., Bacillus cereus, Salmonella spp. and Streptomyces spp. were quantified by real-time PCR. The results indicated that changes in actinobacteria, opposite to those in indicator and harmful bacteria, could be used as indicators for the absence pathogenic bacteria from compost. Still the order of magnitude of Streptomyces spp. in the compost was such high that their spores might be harmful for humans handling the compost material daily, necessitating their protection.

KW - 1172 Environmental sciences

M3 - Chapter

SN - 978-93-84588-09-0

BT - Advances in Biotechnology

A2 - Nawani, Neelu N.

A2 - Khetmalas, Madhukar

A2 - Razdan, P.N.

A2 - Pandey, Ashok

PB - I.K. International Publishing House Pvt. Ltd.

CY - New Delhi

ER -

Kumar SS, Hui N, Kirsi M, Dayanand A, Auvinen P, Laitinen S et al. Advances in Biotechnology: Relation between the actinobacterial succession and microbial safety in aerobic waste treatment (composting). In Nawani NN, Khetmalas M, Razdan PN, Pandey A, editors, Advances in Biotechnology: Relation between the actinobacterial succession and microbial safety in aerobic waste treatment (composting).. New Delhi: I.K. International Publishing House Pvt. Ltd. 2015