An animal model of cutaneous cyst development enables the identification of three QTLs including the homologue of a human locus (TRICY1)

I. Cosgarea, B. Koelsch, C. Fischer, K.G. Griewank, L. van den Berg, A. Kutritz, D. Schadendorf, A. Kindler-Röhrborn

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Brief Summary Using inbred BN and LE/Stm rats susceptible and resistant, respectively, to chemically induced cutaneous cyst development we were able to further unveil the genetic architecture of inherited multiple cyst formation. N-methyl-N-nitrosourea-treated (BN x LE) F2 intercross rats proved to develop differential numbers of cutaneous cysts, demonstrating epidermal, trichilemmal and verrucous keratinization types. Male rats developed significantly more cysts per animal than females. QTL interval mapping yielded three loci on rat chromosomes 1, 8 and 11 (Ccd1, Ccd2, Ccd3) linked to cutaneous cyst formation. Ccd2 proved to be homologous to the human TRICY1 region which could further be narrowed down by genome comparison in both species. It contains 11 genes with evidence of expression in human keratinocytes.
Original languageEnglish
JournalJournal of Investigative Dermatology
ISSN0022-202X
DOIs
Publication statusPublished - 27 Apr 2019
MoE publication typeA1 Journal article-refereed

Cite this

Cosgarea, I. ; Koelsch, B. ; Fischer, C. ; Griewank, K.G. ; van den Berg, L. ; Kutritz, A. ; Schadendorf, D. ; Kindler-Röhrborn, A. / An animal model of cutaneous cyst development enables the identification of three QTLs including the homologue of a human locus (TRICY1). In: Journal of Investigative Dermatology. 2019.
@article{6f9deac298c34253a5ba8203dd68b14c,
title = "An animal model of cutaneous cyst development enables the identification of three QTLs including the homologue of a human locus (TRICY1)",
abstract = "Brief Summary Using inbred BN and LE/Stm rats susceptible and resistant, respectively, to chemically induced cutaneous cyst development we were able to further unveil the genetic architecture of inherited multiple cyst formation. N-methyl-N-nitrosourea-treated (BN x LE) F2 intercross rats proved to develop differential numbers of cutaneous cysts, demonstrating epidermal, trichilemmal and verrucous keratinization types. Male rats developed significantly more cysts per animal than females. QTL interval mapping yielded three loci on rat chromosomes 1, 8 and 11 (Ccd1, Ccd2, Ccd3) linked to cutaneous cyst formation. Ccd2 proved to be homologous to the human TRICY1 region which could further be narrowed down by genome comparison in both species. It contains 11 genes with evidence of expression in human keratinocytes.",
author = "I. Cosgarea and B. Koelsch and C. Fischer and K.G. Griewank and {van den Berg}, L. and A. Kutritz and D. Schadendorf and A. Kindler-R{\"o}hrborn",
year = "2019",
month = "4",
day = "27",
doi = "10.1016/j.jid.2019.03.1154",
language = "English",
journal = "Journal of Investigative Dermatology",
issn = "0022-202X",
publisher = "Nature publishing group",

}

An animal model of cutaneous cyst development enables the identification of three QTLs including the homologue of a human locus (TRICY1). / Cosgarea, I.; Koelsch, B.; Fischer, C.; Griewank, K.G.; van den Berg, L.; Kutritz, A.; Schadendorf, D.; Kindler-Röhrborn, A.

In: Journal of Investigative Dermatology, 27.04.2019.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - An animal model of cutaneous cyst development enables the identification of three QTLs including the homologue of a human locus (TRICY1)

AU - Cosgarea, I.

AU - Koelsch, B.

AU - Fischer, C.

AU - Griewank, K.G.

AU - van den Berg, L.

AU - Kutritz, A.

AU - Schadendorf, D.

AU - Kindler-Röhrborn, A.

PY - 2019/4/27

Y1 - 2019/4/27

N2 - Brief Summary Using inbred BN and LE/Stm rats susceptible and resistant, respectively, to chemically induced cutaneous cyst development we were able to further unveil the genetic architecture of inherited multiple cyst formation. N-methyl-N-nitrosourea-treated (BN x LE) F2 intercross rats proved to develop differential numbers of cutaneous cysts, demonstrating epidermal, trichilemmal and verrucous keratinization types. Male rats developed significantly more cysts per animal than females. QTL interval mapping yielded three loci on rat chromosomes 1, 8 and 11 (Ccd1, Ccd2, Ccd3) linked to cutaneous cyst formation. Ccd2 proved to be homologous to the human TRICY1 region which could further be narrowed down by genome comparison in both species. It contains 11 genes with evidence of expression in human keratinocytes.

AB - Brief Summary Using inbred BN and LE/Stm rats susceptible and resistant, respectively, to chemically induced cutaneous cyst development we were able to further unveil the genetic architecture of inherited multiple cyst formation. N-methyl-N-nitrosourea-treated (BN x LE) F2 intercross rats proved to develop differential numbers of cutaneous cysts, demonstrating epidermal, trichilemmal and verrucous keratinization types. Male rats developed significantly more cysts per animal than females. QTL interval mapping yielded three loci on rat chromosomes 1, 8 and 11 (Ccd1, Ccd2, Ccd3) linked to cutaneous cyst formation. Ccd2 proved to be homologous to the human TRICY1 region which could further be narrowed down by genome comparison in both species. It contains 11 genes with evidence of expression in human keratinocytes.

U2 - 10.1016/j.jid.2019.03.1154

DO - 10.1016/j.jid.2019.03.1154

M3 - Article

JO - Journal of Investigative Dermatology

JF - Journal of Investigative Dermatology

SN - 0022-202X

ER -