An efflux transporter PbrA and a phosphatase PbrB cooperate in a lead-resistance mechanism in bacteria

Anu Hynninen, Thierry Touzé, Leena Pitkänen, Dominique Mengin-Lecreulx , Marko Petri Juhani Virta

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The gene cluster pbrTRABCD from Cupriavidus metallidurans CH34 is thought to encode a unique, specific resistance mechanism for lead. However, the exact functions of these genes are unknown. In this study we examine the metal specificity and functions of pbrABCD by expressing these genes in different combinations and comparing their ability to restore Pb2+, Zn2+ and Cd2+ resistance in a metal-sensitive C. metallidurans strain DN440. We show that lead resistance in C. metallidurans is achieved through the cooperation of the Zn/Cd/Pb-translocating ATPase PbrA and the undecaprenyl pyrophosphate phosphatase PbrB. While PbrA non-specifically exported Pb2+, Zn2+ and Cd2+, a specific increase in lead resistance was observed when PbrA and PbrB were coexpressed. As a model of action for PbrA and PbrB we propose a mechanism where Pb2+ is exported from the cytoplasm by PbrA and then sequestered as a phosphate salt with the inorganic phosphate produced by PbrB. Similar operons containing genes for heavy metal translocating ATPases and phosphatases were found in several different bacterial species, suggesting that lead detoxification through active efflux and sequestration is a common lead-resistance mechanism.
Original languageEnglish
JournalMolecular Microbiology
Volume74
Issue number2
Pages (from-to)384-394
Number of pages11
ISSN0950-382X
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1182 Biochemistry, cell and molecular biology

Cite this

Hynninen, Anu ; Touzé, Thierry ; Pitkänen, Leena ; Mengin-Lecreulx , Dominique ; Virta, Marko Petri Juhani. / An efflux transporter PbrA and a phosphatase PbrB cooperate in a lead-resistance mechanism in bacteria. In: Molecular Microbiology. 2009 ; Vol. 74, No. 2. pp. 384-394.
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abstract = "The gene cluster pbrTRABCD from Cupriavidus metallidurans CH34 is thought to encode a unique, specific resistance mechanism for lead. However, the exact functions of these genes are unknown. In this study we examine the metal specificity and functions of pbrABCD by expressing these genes in different combinations and comparing their ability to restore Pb2+, Zn2+ and Cd2+ resistance in a metal-sensitive C. metallidurans strain DN440. We show that lead resistance in C. metallidurans is achieved through the cooperation of the Zn/Cd/Pb-translocating ATPase PbrA and the undecaprenyl pyrophosphate phosphatase PbrB. While PbrA non-specifically exported Pb2+, Zn2+ and Cd2+, a specific increase in lead resistance was observed when PbrA and PbrB were coexpressed. As a model of action for PbrA and PbrB we propose a mechanism where Pb2+ is exported from the cytoplasm by PbrA and then sequestered as a phosphate salt with the inorganic phosphate produced by PbrB. Similar operons containing genes for heavy metal translocating ATPases and phosphatases were found in several different bacterial species, suggesting that lead detoxification through active efflux and sequestration is a common lead-resistance mechanism.",
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An efflux transporter PbrA and a phosphatase PbrB cooperate in a lead-resistance mechanism in bacteria. / Hynninen, Anu; Touzé, Thierry; Pitkänen, Leena; Mengin-Lecreulx , Dominique; Virta, Marko Petri Juhani.

In: Molecular Microbiology, Vol. 74, No. 2, 2009, p. 384-394.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - An efflux transporter PbrA and a phosphatase PbrB cooperate in a lead-resistance mechanism in bacteria

AU - Hynninen, Anu

AU - Touzé, Thierry

AU - Pitkänen, Leena

AU - Mengin-Lecreulx , Dominique

AU - Virta, Marko Petri Juhani

PY - 2009

Y1 - 2009

N2 - The gene cluster pbrTRABCD from Cupriavidus metallidurans CH34 is thought to encode a unique, specific resistance mechanism for lead. However, the exact functions of these genes are unknown. In this study we examine the metal specificity and functions of pbrABCD by expressing these genes in different combinations and comparing their ability to restore Pb2+, Zn2+ and Cd2+ resistance in a metal-sensitive C. metallidurans strain DN440. We show that lead resistance in C. metallidurans is achieved through the cooperation of the Zn/Cd/Pb-translocating ATPase PbrA and the undecaprenyl pyrophosphate phosphatase PbrB. While PbrA non-specifically exported Pb2+, Zn2+ and Cd2+, a specific increase in lead resistance was observed when PbrA and PbrB were coexpressed. As a model of action for PbrA and PbrB we propose a mechanism where Pb2+ is exported from the cytoplasm by PbrA and then sequestered as a phosphate salt with the inorganic phosphate produced by PbrB. Similar operons containing genes for heavy metal translocating ATPases and phosphatases were found in several different bacterial species, suggesting that lead detoxification through active efflux and sequestration is a common lead-resistance mechanism.

AB - The gene cluster pbrTRABCD from Cupriavidus metallidurans CH34 is thought to encode a unique, specific resistance mechanism for lead. However, the exact functions of these genes are unknown. In this study we examine the metal specificity and functions of pbrABCD by expressing these genes in different combinations and comparing their ability to restore Pb2+, Zn2+ and Cd2+ resistance in a metal-sensitive C. metallidurans strain DN440. We show that lead resistance in C. metallidurans is achieved through the cooperation of the Zn/Cd/Pb-translocating ATPase PbrA and the undecaprenyl pyrophosphate phosphatase PbrB. While PbrA non-specifically exported Pb2+, Zn2+ and Cd2+, a specific increase in lead resistance was observed when PbrA and PbrB were coexpressed. As a model of action for PbrA and PbrB we propose a mechanism where Pb2+ is exported from the cytoplasm by PbrA and then sequestered as a phosphate salt with the inorganic phosphate produced by PbrB. Similar operons containing genes for heavy metal translocating ATPases and phosphatases were found in several different bacterial species, suggesting that lead detoxification through active efflux and sequestration is a common lead-resistance mechanism.

KW - 1182 Biochemistry, cell and molecular biology

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JF - Molecular Microbiology

SN - 0950-382X

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