An unusual structure at one end of potato potyvirus particles

Lesley Torrance, Igor A Andreev, Rasa Gabrenaite-Verhovskaya, Graham Cowan, Kristiina Mäkinen, Michael E Taliansky

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The particles of potato virus Y (PVY) and potato virus A (PVA) potyviruses are helically constructed filaments that are thought to contain a single type of coat protein subunit. Examination of negatively stained virions by electron microscopy reveals flexuous rod-shaped particles with no obvious terminal particles incorporate additional minor protein components, which form stable complexes that mediate particle disassembly, movement or transmission by vectors. Some of this information has been obtained using imaging techniques such as atomic force microscopy. The particles of PVY and PVA were examined by atomic force microscopy and immunogold labelling electron microscopy. Our results show that some of the potyvirus particles contain a protruding tip at one end of the virus particles, which is presumably associated with the 5'-end of viral RNA. The lip contains the virus-encoded proteins genome-linked protein and helper-component proteinase. The composition and possible roles of the terminal tip structures in virus biology are discussed. (c) 2005 Elsevier Ltd. All rights reserved.
Original languageEnglish
JournalJournal of Molecular Biology
Volume357
Issue number1
Pages (from-to)1-8
Number of pages8
ISSN0022-2836
DOIs
Publication statusPublished - 2006
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 414 Agricultural biotechnology
  • kasvitaudit
  • 411 Agriculture and forestry

Cite this

Torrance, L., Andreev, I. A., Gabrenaite-Verhovskaya, R., Cowan, G., Mäkinen, K., & Taliansky, M. E. (2006). An unusual structure at one end of potato potyvirus particles. Journal of Molecular Biology, 357(1), 1-8. https://doi.org/10.1016/j.jmb.2005.12.021
Torrance, Lesley ; Andreev, Igor A ; Gabrenaite-Verhovskaya, Rasa ; Cowan, Graham ; Mäkinen, Kristiina ; Taliansky, Michael E. / An unusual structure at one end of potato potyvirus particles. In: Journal of Molecular Biology. 2006 ; Vol. 357, No. 1. pp. 1-8.
@article{24943981c8c64319afb3092dee53242c,
title = "An unusual structure at one end of potato potyvirus particles",
abstract = "The particles of potato virus Y (PVY) and potato virus A (PVA) potyviruses are helically constructed filaments that are thought to contain a single type of coat protein subunit. Examination of negatively stained virions by electron microscopy reveals flexuous rod-shaped particles with no obvious terminal particles incorporate additional minor protein components, which form stable complexes that mediate particle disassembly, movement or transmission by vectors. Some of this information has been obtained using imaging techniques such as atomic force microscopy. The particles of PVY and PVA were examined by atomic force microscopy and immunogold labelling electron microscopy. Our results show that some of the potyvirus particles contain a protruding tip at one end of the virus particles, which is presumably associated with the 5'-end of viral RNA. The lip contains the virus-encoded proteins genome-linked protein and helper-component proteinase. The composition and possible roles of the terminal tip structures in virus biology are discussed. (c) 2005 Elsevier Ltd. All rights reserved.",
keywords = "414 Agricultural biotechnology, kasvitaudit, kasvitaudit, kasvitaudit, 411 Agriculture and forestry",
author = "Lesley Torrance and Andreev, {Igor A} and Rasa Gabrenaite-Verhovskaya and Graham Cowan and Kristiina M{\"a}kinen and Taliansky, {Michael E}",
year = "2006",
doi = "10.1016/j.jmb.2005.12.021",
language = "English",
volume = "357",
pages = "1--8",
journal = "Journal of Molecular Biology",
issn = "0022-2836",
publisher = "ACADEMIC PRESS INC ELSEVIER SCIENCE",
number = "1",

}

Torrance, L, Andreev, IA, Gabrenaite-Verhovskaya, R, Cowan, G, Mäkinen, K & Taliansky, ME 2006, 'An unusual structure at one end of potato potyvirus particles', Journal of Molecular Biology, vol. 357, no. 1, pp. 1-8. https://doi.org/10.1016/j.jmb.2005.12.021

An unusual structure at one end of potato potyvirus particles. / Torrance, Lesley; Andreev, Igor A; Gabrenaite-Verhovskaya, Rasa; Cowan, Graham; Mäkinen, Kristiina; Taliansky, Michael E.

In: Journal of Molecular Biology, Vol. 357, No. 1, 2006, p. 1-8.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - An unusual structure at one end of potato potyvirus particles

AU - Torrance, Lesley

AU - Andreev, Igor A

AU - Gabrenaite-Verhovskaya, Rasa

AU - Cowan, Graham

AU - Mäkinen, Kristiina

AU - Taliansky, Michael E

PY - 2006

Y1 - 2006

N2 - The particles of potato virus Y (PVY) and potato virus A (PVA) potyviruses are helically constructed filaments that are thought to contain a single type of coat protein subunit. Examination of negatively stained virions by electron microscopy reveals flexuous rod-shaped particles with no obvious terminal particles incorporate additional minor protein components, which form stable complexes that mediate particle disassembly, movement or transmission by vectors. Some of this information has been obtained using imaging techniques such as atomic force microscopy. The particles of PVY and PVA were examined by atomic force microscopy and immunogold labelling electron microscopy. Our results show that some of the potyvirus particles contain a protruding tip at one end of the virus particles, which is presumably associated with the 5'-end of viral RNA. The lip contains the virus-encoded proteins genome-linked protein and helper-component proteinase. The composition and possible roles of the terminal tip structures in virus biology are discussed. (c) 2005 Elsevier Ltd. All rights reserved.

AB - The particles of potato virus Y (PVY) and potato virus A (PVA) potyviruses are helically constructed filaments that are thought to contain a single type of coat protein subunit. Examination of negatively stained virions by electron microscopy reveals flexuous rod-shaped particles with no obvious terminal particles incorporate additional minor protein components, which form stable complexes that mediate particle disassembly, movement or transmission by vectors. Some of this information has been obtained using imaging techniques such as atomic force microscopy. The particles of PVY and PVA were examined by atomic force microscopy and immunogold labelling electron microscopy. Our results show that some of the potyvirus particles contain a protruding tip at one end of the virus particles, which is presumably associated with the 5'-end of viral RNA. The lip contains the virus-encoded proteins genome-linked protein and helper-component proteinase. The composition and possible roles of the terminal tip structures in virus biology are discussed. (c) 2005 Elsevier Ltd. All rights reserved.

KW - 414 Agricultural biotechnology

KW - kasvitaudit

KW - kasvitaudit

KW - kasvitaudit

KW - 411 Agriculture and forestry

U2 - 10.1016/j.jmb.2005.12.021

DO - 10.1016/j.jmb.2005.12.021

M3 - Article

VL - 357

SP - 1

EP - 8

JO - Journal of Molecular Biology

JF - Journal of Molecular Biology

SN - 0022-2836

IS - 1

ER -