Analysis of phloem trajectory links tissue maturation to cell specialization.

Pawel Roszak; Jungok Heo; Bernhard Blob; Koichi Toyokura; Maria Angels De Luis Balaguer; Winnie Lau; Fiona K Hamey; Jacopo  Cirrone; Xin Wang; Robertas Ursache; Hugo Tavares; Kevin Verstaen; Jos Wendrich; Charles W. Melnyk; Dennis Shasha; Sebastian  Ahnert; Yvan Saeys; Bert De Rybel; Renze Heidstra; Ben  Scheres; Ari Pekka Mähönen; Berthold Gottgens; Rosangela Sozzani; Kenneth David  Birnbaum; Yrjö Helariutta

Analysis of phloem trajectory links tissue maturation to cell specialization.

Pawel Roszak, Jungok Heo, Bernhard Blob, Koichi Toyokura, Maria Angels De Luis Balaguer, Winnie Lau, Fiona K Hamey, Jacopo Cirrone, Xin Wang, Robertas Ursache, Hugo Tavares, Kevin Verstaen, Jos Wendrich, Charles W. Melnyk, Dennis Shasha, Sebastian Ahnert, Yvan Saeys, Bert De Rybel, Renze Heidstra, Ben ScheresAri Pekka Mähönen, Berthold Gottgens, Rosangela Sozzani, Kenneth David Birnbaum, Yrjö Helariutta

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

The mechanisms that allow cells in the plant meristem to coordinate tissue-wide maturation gradients with specialized cell networks are critical for indeterminate growth. Here, we reconstructed the protophloem developmental trajectory of 19 cells from cell birth to terminal differentiation at single cell resolution in the Arabidopsis root. We found that cellular specification is mediated near the stem cell niche by PHLOEM EARLY DOF (PEAR) transcription factors. However, the PEAR dependent differentiation program is repressed by a broad gradient of PLETHORA (PLT) transcription factors, which directly inhibit PEARs' own direct target ALTERED PHLOEM DEVELOPMENT (APL). The dissipation of PLT gradient around 7 cells from the stem cell activates APL expression, and a subsequent transitional network that results in a "seesaw" pattern of mutual inhibition over developmental time. Together, we provide a mechanistic understanding of how morphogen-like maturation gradients interface with cell-type specific transcriptional regulators to stage cellular differentiation.

Original language	English
Journal	bioRxiv : the preprint server for biology
Publication status	Unpublished - 19 Jan 2021
MoE publication type	A1 Journal article-refereed

Cite this

@article{90ce15768db1479085d40a5588525b09,

title = "Analysis of phloem trajectory links tissue maturation to cell specialization.",

abstract = "The mechanisms that allow cells in the plant meristem to coordinate tissue-wide maturation gradients with specialized cell networks are critical for indeterminate growth. Here, we reconstructed the protophloem developmental trajectory of 19 cells from cell birth to terminal differentiation at single cell resolution in the Arabidopsis root. We found that cellular specification is mediated near the stem cell niche by PHLOEM EARLY DOF (PEAR) transcription factors. However, the PEAR dependent differentiation program is repressed by a broad gradient of PLETHORA (PLT) transcription factors, which directly inhibit PEARs' own direct target ALTERED PHLOEM DEVELOPMENT (APL). The dissipation of PLT gradient around 7 cells from the stem cell activates APL expression, and a subsequent transitional network that results in a {"}seesaw{"} pattern of mutual inhibition over developmental time. Together, we provide a mechanistic understanding of how morphogen-like maturation gradients interface with cell-type specific transcriptional regulators to stage cellular differentiation.",

author = "Pawel Roszak and Jungok Heo and Bernhard Blob and Koichi Toyokura and {De Luis Balaguer}, {Maria Angels} and Winnie Lau and Hamey, {Fiona K} and Jacopo Cirrone and Xin Wang and Robertas Ursache and Hugo Tavares and Kevin Verstaen and Jos Wendrich and Melnyk, {Charles W.} and Dennis Shasha and Sebastian Ahnert and Yvan Saeys and {De Rybel}, Bert and Renze Heidstra and Ben Scheres and M{\"a}h{\"o}nen, {Ari Pekka} and Berthold Gottgens and Rosangela Sozzani and Birnbaum, {Kenneth David} and Yrj{\"o} Helariutta",

year = "2021",

month = jan,

day = "19",

language = "English",

journal = "bioRxiv : the preprint server for biology ",

publisher = "Cold Spring Harbor Laboratory",

}

Roszak, P, Heo, J, Blob, B, Toyokura, K, De Luis Balaguer, MA, Lau, W, Hamey, FK, Cirrone, J, Wang, X, Ursache, R, Tavares, H, Verstaen, K, Wendrich, J, Melnyk, CW, Shasha, D, Ahnert, S, Saeys, Y, De Rybel, B, Heidstra, R, Scheres, B, Mähönen, AP, Gottgens, B, Sozzani, R, Birnbaum, KD & Helariutta, Y 2021, 'Analysis of phloem trajectory links tissue maturation to cell specialization.', bioRxiv : the preprint server for biology .

TY - JOUR

T1 - Analysis of phloem trajectory links tissue maturation to cell specialization.

AU - Roszak, Pawel

AU - Heo, Jungok

AU - Blob, Bernhard

AU - Toyokura, Koichi

AU - De Luis Balaguer, Maria Angels

AU - Lau, Winnie

AU - Hamey, Fiona K

AU - Cirrone, Jacopo

AU - Wang, Xin

AU - Ursache, Robertas

AU - Tavares, Hugo

AU - Verstaen, Kevin

AU - Wendrich, Jos

AU - Melnyk, Charles W.

AU - Shasha, Dennis

AU - Ahnert, Sebastian

AU - Saeys, Yvan

AU - De Rybel, Bert

AU - Heidstra, Renze

AU - Scheres, Ben

AU - Mähönen, Ari Pekka

AU - Gottgens, Berthold

AU - Sozzani, Rosangela

AU - Birnbaum, Kenneth David

AU - Helariutta, Yrjö

PY - 2021/1/19

Y1 - 2021/1/19

N2 - The mechanisms that allow cells in the plant meristem to coordinate tissue-wide maturation gradients with specialized cell networks are critical for indeterminate growth. Here, we reconstructed the protophloem developmental trajectory of 19 cells from cell birth to terminal differentiation at single cell resolution in the Arabidopsis root. We found that cellular specification is mediated near the stem cell niche by PHLOEM EARLY DOF (PEAR) transcription factors. However, the PEAR dependent differentiation program is repressed by a broad gradient of PLETHORA (PLT) transcription factors, which directly inhibit PEARs' own direct target ALTERED PHLOEM DEVELOPMENT (APL). The dissipation of PLT gradient around 7 cells from the stem cell activates APL expression, and a subsequent transitional network that results in a "seesaw" pattern of mutual inhibition over developmental time. Together, we provide a mechanistic understanding of how morphogen-like maturation gradients interface with cell-type specific transcriptional regulators to stage cellular differentiation.

AB - The mechanisms that allow cells in the plant meristem to coordinate tissue-wide maturation gradients with specialized cell networks are critical for indeterminate growth. Here, we reconstructed the protophloem developmental trajectory of 19 cells from cell birth to terminal differentiation at single cell resolution in the Arabidopsis root. We found that cellular specification is mediated near the stem cell niche by PHLOEM EARLY DOF (PEAR) transcription factors. However, the PEAR dependent differentiation program is repressed by a broad gradient of PLETHORA (PLT) transcription factors, which directly inhibit PEARs' own direct target ALTERED PHLOEM DEVELOPMENT (APL). The dissipation of PLT gradient around 7 cells from the stem cell activates APL expression, and a subsequent transitional network that results in a "seesaw" pattern of mutual inhibition over developmental time. Together, we provide a mechanistic understanding of how morphogen-like maturation gradients interface with cell-type specific transcriptional regulators to stage cellular differentiation.

UR - https://www.biorxiv.org/content/10.1101/2021.01.18.427084v1

M3 - Article

JO - bioRxiv : the preprint server for biology

JF - bioRxiv : the preprint server for biology

ER -

Analysis of phloem trajectory links tissue maturation to cell specialization.

Abstract

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