Anthocyanin biosynthesis in gerbera cultivar ‘Estelle’ and its acyanic sport ‘Ivory’

Hany Bashandy, Milla Irene Pietiäinen, Elisabete Carvalho, Kean-Jin Lim, Paula Riitta Elomaa, Stefan Martens, Teemu Heikki Teeri

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Flavonoids in the model ornamental plant Gerbera hybrida consist of flavones, flavonols and anthocyanins. Anthocyanins accumulate in the adaxial epidermis of petals and give the different cultivars their characteristic red and violet colour. Both pelargonidin and cyanidin derivatives are found in gerbera, but none of the cultivars contain delphinidin. ‘Ivory’, a cultivar with white petals, is a sport of the pelargonidin-containing pink cultivar ‘Estelle’, i.e. it originates from an acyanic branch of ‘Estelle’. In this work, four different alleles encoding dihydroflavonol 4-reductase (DFR) were identified in gerbera cultivars. We found that, in contrast to ‘Estelle’ with the functional allele GDFR1–2, ‘Ivory’ carries a mutation in this gene that results in an inactive enzyme. Interestingly, ‘Ivory’ also expresses a second, nonmutated allele (GDFR1–3) in petal epidermi, leading to extractable DFR activity but not to anthocyanin biosynthesis. The second allele encodes a protein identical in amino acid sequence to the DFR of the cyanidin-containing variety ‘President’. Pelargonidin-containing cultivars do not react to the flavonoid 3′-hydroxylase inhibitor tetcyclacis, but cyanidin-containing cultivars lose their colour, instead of starting to synthesise pelargonidins, indicating the specificity of GDFR1-3 for the cyanidin pathway. This explains why petals of ‘Ivory’ are white, even when it has lost only one of the two enzymatically functional DFR forms, and shows that anthocyanin biosynthesis in gerbera is under more complex regulation than earlier thought.
Original languageEnglish
JournalPlanta
Volume242
Issue number3
Pages (from-to)601-611
Number of pages11
ISSN0032-0935
DOIs
Publication statusPublished - 2015
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1181 Ecology, evolutionary biology
  • 4111 Agronomy
  • 414 Agricultural biotechnology

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