Apolipoprotein E activates the low-activity form of human phospholipid transfer protein

Minna T Jänis, Jari Metso, Hilkka Lankinen, Tomas Strandin, Vesa M Olkkonen, Kerry-Anne Rye, Matti Jauhiainen, Christian Ehnholm

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    "Phospholipid transfer protein (PLTP) exists in a high-activity (HA-PLTP) and a low-activity form (LA-PLTP) in the circulation. LA-PLTP is associated with apoA-I while the HA-PLTP complex is enriched with apoE. To study the interaction of PLTP with apolipoproteins, we carried out surface plasmon resonance analyses. These demonstrated a concentration-dependent binding of recombinant human PLTP, which represents an active PLTP form, and LA-PLTP to apoE, apoA-I, and apoA-IV within a nanomolar K-D range. To study whether LA-PLTP can be transformed into an active form, we incubated it in the presence of proteoliposomes containing apoE, apoA-I or apoA-IV. The apoE proteoliposomes induced a concentration-dependent activation of LA-PLTP. ApoA-IV proteoliposomes also activated LA-PLTP in a concentration-dependent manner, whereas apoA-I proteoliposomes had no such effect. These observations suggest that PLTP is capable of interacting with apoE, apoA-L and apoA-IV. and that these interactions regulate PLTP-activity levels in plasma. © 2005 Elsevier Inc. All rights reserved."
    Original languageEnglish
    JournalBiochemical and Biophysical Research Communications
    Volume331
    Issue number1
    Pages (from-to)333-340
    Number of pages8
    ISSN0006-291X
    DOIs
    Publication statusPublished - 2005
    MoE publication typeA1 Journal article-refereed

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