Application of array-comparative genomic hybridization analysis in immune-virotherapy approach

A. Vitale, C. Capasso, E. Leggiero, M. Garofalo, L. Kuryk, M. Hirvinen, F. D’Alessio, C. Perrotta, F. Verdesca, A. Ranieri, F. Salvatore, L. Pastore, P. Buono, V. Cerullo, B. Lombardo

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Introduction: oncololytic adenoviruses (OAds), viruses constructed to replicate in tumor cells, improve the outcome of cancer therapy in some cases, such as sarcomas. However, the molecular heterogeneity of tumors requires specific and personalized cancer treatments in order to set up more adequate and effective therapies. Methods: by using the array Comparative Genomic Hybridization (array-CGH), a molecular approach method, we aimed to identify chromosomal aberrations or Copy Number Variants (CNVs) in three different tumor cell lines: HCT116, SW872 and A2058 selected for their Coxsackievirus and Adenovirus receptor (CAR) expression profile. Results: the cells showed several duplications of genes involved in replicative Adenovirus cycle (binding, internalization, escape) in the core transport, and in the escape of the viral DNA from the capsid. Conclusion: in this study, our aim was to identify chromosomal alterations in genes involved in the OAd replication cycle process. Array-CGH method could be useful to design a platform for a screening analysis in order to identify mutations that can contribute to oncolytic virotherapy approach generating a personalized strategy for tumor suppression. © 2020 Biomedia. All rights reserved.
Original languageEnglish
JournalBiochimica Clinica
Volume44
Issue number1
Pages (from-to)61-67
Number of pages7
ISSN0393-0564
Publication statusPublished - 2020
MoE publication typeA1 Journal article-refereed

Fields of Science

  • coxsackie virus and adenovirus receptor
  • virus DNA
  • A2058 cell line
  • Adenoviridae
  • Article
  • chromosome aberration
  • comparative genomic hybridization
  • controlled study
  • copy number variation
  • gene duplication
  • gene expression
  • HCT 116 cell line
  • human
  • human cell
  • nonhuman
  • oncolytic virotherapy
  • SW872 cell line
  • virus attachment
  • virus capsid
  • virus entry
  • 11832 Microbiology and virology

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