Assessment of Immunogenicity and Efficacy of a Zika Vaccine Using Modified Vaccinia Ankara Virus as Carriers.

César López-Camacho, Young Chan Kim, Peter Abbink, Rafael A Larocca, Juha T. Huiskonen, Dan H. Barouch, Arturo Reyes-Sandoval

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Abstract

Zika virus (ZIKV) is an emerging mosquito-borne flavivirus that has spread to more than 70 countries worldwide since 2015. Despite active research, there are currently no licensed vaccines or therapeutics. We have previously reported the development of various adenoviral vectored vaccine candidates (ChAdOx1 ZIKV) with the ability to stimulate effective immunity in mice and provide protection upon a ZIKV challenge model, using a non-adjuvanted single vaccination approach. In this study, we constructed various modified vaccinia Ankara (MVA) viruses to express the ZIKV Envelope (E) with modifications on the precursor membrane (prM) or on the C-terminus envelope transmembrane domain (TM), similar to our ChAdOx1 vaccine candidates. MVA-ZIKV vaccine candidates were evaluated as a non-adjuvanted single vaccination regimen against a ZIKV Brazilian isolate, using viraemia as the correlate of protection. Here, we report the induction of a modest level of anti-ZIKV E antibodies by all MVA vectored vaccines and sub-optimal efficacy in a ZIKV challenge model. Our results indicate the requirement of additional strategies when using MVA-ZIKV vaccines to afford sterile protection upon a non-adjuvanted and single vaccination regime.
Original languageEnglish
Article numberE216
JournalPathogens
Volume8
Issue number4
Number of pages11
ISSN2076-0817
DOIs
Publication statusPublished - 2 Nov 2019
Externally publishedYes
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1182 Biochemistry, cell and molecular biology
  • 3111 Biomedicine

Cite this

López-Camacho, C., Kim, Y. C., Abbink, P., Larocca, R. A., Huiskonen, J. T., Barouch, D. H., & Reyes-Sandoval, A. (2019). Assessment of Immunogenicity and Efficacy of a Zika Vaccine Using Modified Vaccinia Ankara Virus as Carriers. Pathogens, 8(4), [E216]. https://doi.org/10.3390/pathogens8040216