Automatic methodologies to perform loading and release assays of anticancer drugs from mesoporous silicon nanoparticles

Sarah Pereira, Marieta Passos, Alexandra Correia, Ermei Mäkilä, Jarno Salonen, Andrá Araujo, Helder Almeida Santos, M. Lúcia Saraiva

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Two automatic methodologies were developed to perform the loading and release assays of drugs from porous silicon (PSi) nanoparticles. 5-Fluorouracil (5-FU) was selected as a model drug, and different functionalized PSi nanoparticles were used. While for drug loading studies the reproducible characteristics of flow systems were explored regarding mixture and volumes taken, in the drug release flow methodology versatility in accommodating different devices around the valve were tested. Fluorescent properties of 5-FU were used with detection limit of 9×10−4 mg L−1 and a linear response up to 5 mg L−1 The drug loading and release procedures were optimized in sequential injection analysis (SIA) systems obtaining a huge economy regarding the time spent (4 min towards 2 hours). Batch and SIA methods were tested and compared for the different behaviours of the PSi nanoparticles towards both methodologies and no noteworthy differences were obtained with Student's t-test for loading with a calculated t value of 2.04 showing the absence of statistical differences. All the results present a RSD less than 10%. So, the developed automatic methodologies are a viable alternative to load drugs and to study the release profiles from PSi nanoparticles.
Original languageEnglish
JournalTalanta
Volume196
Pages (from-to)277-283
Number of pages7
ISSN0039-9140
DOIs
Publication statusPublished - 1 May 2019
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 5-fluorouracil
  • CARBONIZED POROUS SILICON
  • DELIVERY
  • DISSOLUTION
  • FLUOROMETRIC-DETERMINATION
  • INJECTION SPECTROPHOTOMETRIC DETERMINATION
  • Loading
  • MICROPARTICLES
  • Mesoporous silicon nanoparticles
  • Release
  • SEQUENTIAL INJECTION
  • SURFACE
  • Sequential injection analysis
  • 116 Chemical sciences
  • 221 Nano-technology
  • 317 Pharmacy

Cite this

Pereira, Sarah ; Passos, Marieta ; Correia, Alexandra ; Mäkilä, Ermei ; Salonen, Jarno ; Araujo, Andrá ; Almeida Santos, Helder ; Saraiva, M. Lúcia . / Automatic methodologies to perform loading and release assays of anticancer drugs from mesoporous silicon nanoparticles. In: Talanta. 2019 ; Vol. 196. pp. 277-283.
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abstract = "Two automatic methodologies were developed to perform the loading and release assays of drugs from porous silicon (PSi) nanoparticles. 5-Fluorouracil (5-FU) was selected as a model drug, and different functionalized PSi nanoparticles were used. While for drug loading studies the reproducible characteristics of flow systems were explored regarding mixture and volumes taken, in the drug release flow methodology versatility in accommodating different devices around the valve were tested. Fluorescent properties of 5-FU were used with detection limit of 9×10−4 mg L−1 and a linear response up to 5 mg L−1 The drug loading and release procedures were optimized in sequential injection analysis (SIA) systems obtaining a huge economy regarding the time spent (4 min towards 2 hours). Batch and SIA methods were tested and compared for the different behaviours of the PSi nanoparticles towards both methodologies and no noteworthy differences were obtained with Student's t-test for loading with a calculated t value of 2.04 showing the absence of statistical differences. All the results present a RSD less than 10{\%}. So, the developed automatic methodologies are a viable alternative to load drugs and to study the release profiles from PSi nanoparticles.",
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author = "Sarah Pereira and Marieta Passos and Alexandra Correia and Ermei M{\"a}kil{\"a} and Jarno Salonen and Andr{\'a} Araujo and {Almeida Santos}, Helder and Saraiva, {M. L{\'u}cia}",
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Automatic methodologies to perform loading and release assays of anticancer drugs from mesoporous silicon nanoparticles. / Pereira, Sarah; Passos, Marieta; Correia, Alexandra ; Mäkilä, Ermei; Salonen, Jarno; Araujo, Andrá; Almeida Santos, Helder; Saraiva, M. Lúcia .

In: Talanta, Vol. 196, 01.05.2019, p. 277-283.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Automatic methodologies to perform loading and release assays of anticancer drugs from mesoporous silicon nanoparticles

AU - Pereira, Sarah

AU - Passos, Marieta

AU - Correia, Alexandra

AU - Mäkilä, Ermei

AU - Salonen, Jarno

AU - Araujo, Andrá

AU - Almeida Santos, Helder

AU - Saraiva, M. Lúcia

PY - 2019/5/1

Y1 - 2019/5/1

N2 - Two automatic methodologies were developed to perform the loading and release assays of drugs from porous silicon (PSi) nanoparticles. 5-Fluorouracil (5-FU) was selected as a model drug, and different functionalized PSi nanoparticles were used. While for drug loading studies the reproducible characteristics of flow systems were explored regarding mixture and volumes taken, in the drug release flow methodology versatility in accommodating different devices around the valve were tested. Fluorescent properties of 5-FU were used with detection limit of 9×10−4 mg L−1 and a linear response up to 5 mg L−1 The drug loading and release procedures were optimized in sequential injection analysis (SIA) systems obtaining a huge economy regarding the time spent (4 min towards 2 hours). Batch and SIA methods were tested and compared for the different behaviours of the PSi nanoparticles towards both methodologies and no noteworthy differences were obtained with Student's t-test for loading with a calculated t value of 2.04 showing the absence of statistical differences. All the results present a RSD less than 10%. So, the developed automatic methodologies are a viable alternative to load drugs and to study the release profiles from PSi nanoparticles.

AB - Two automatic methodologies were developed to perform the loading and release assays of drugs from porous silicon (PSi) nanoparticles. 5-Fluorouracil (5-FU) was selected as a model drug, and different functionalized PSi nanoparticles were used. While for drug loading studies the reproducible characteristics of flow systems were explored regarding mixture and volumes taken, in the drug release flow methodology versatility in accommodating different devices around the valve were tested. Fluorescent properties of 5-FU were used with detection limit of 9×10−4 mg L−1 and a linear response up to 5 mg L−1 The drug loading and release procedures were optimized in sequential injection analysis (SIA) systems obtaining a huge economy regarding the time spent (4 min towards 2 hours). Batch and SIA methods were tested and compared for the different behaviours of the PSi nanoparticles towards both methodologies and no noteworthy differences were obtained with Student's t-test for loading with a calculated t value of 2.04 showing the absence of statistical differences. All the results present a RSD less than 10%. So, the developed automatic methodologies are a viable alternative to load drugs and to study the release profiles from PSi nanoparticles.

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KW - DELIVERY

KW - DISSOLUTION

KW - FLUOROMETRIC-DETERMINATION

KW - INJECTION SPECTROPHOTOMETRIC DETERMINATION

KW - Loading

KW - MICROPARTICLES

KW - Mesoporous silicon nanoparticles

KW - Release

KW - SEQUENTIAL INJECTION

KW - SURFACE

KW - Sequential injection analysis

KW - 116 Chemical sciences

KW - 221 Nano-technology

KW - 317 Pharmacy

U2 - 10.1016/j.talanta.2018.12.025

DO - 10.1016/j.talanta.2018.12.025

M3 - Article

VL - 196

SP - 277

EP - 283

JO - Talanta

JF - Talanta

SN - 0039-9140

ER -