Carbonic anhydrase IX is highly expressed in hereditary nonpolyposis colorectal cancer

Anssi M Niemela, Piritta Hynninen, Jukka-Pekka Mecklin, Teijo Kuopio, Antti Kokko, Lauri A Aaltonen, Anna-Kaisa Parkkila, Silvia Pastorekova, Jaromir Pastorek, Abdul Waheed, William S Sly, Torben F Ørntoft, Mogens Kruhøffer, Hannu Haapasalo, Seppo Parkkila, Antti Kivelä

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    Carbonic anhydrase (CA) II, CA IX, and CA XII are expressed in various neoplasias and have been linked to tumorigenesis. We examined their expression in three different groups of colorectal cancer [i.e., microsatellite stable (MSS), microsatellite instable (MSI), and hereditary nonpolyposis colorectal cancer (HNPCC)]. First, we analyzed gene expression profiles of 113 specimens by a microarray method to study the expression of various CA isozymes in the subgroups of colorectal cancer. The results indicated that mRNAs for CA II and CA XII are down-regulated and CA IX mRNA is up-regulated in all three tumor categories when compared with the normal tissue. The up-regulation of CA IX was greatest in the HNPCC group. For more information, 77 specimens were immunohistochemically stained to study the levels of CA II, CA IX, and CA XII immunohistochemical analyses further confirmed that the subgroups express CA II, CA IX, and CA XII differentially, and the HNPCC tumors express high levels of CA IX Expression of these CAs did not correlate to Dukes stage or grade of differentiation. Our results show that CAs are differentially expressed in the subgroups of colorectal cancer, and CA IX expression seems to be very high in most cases of HNPCC. CA IX could be a potential diagnostic and therapeutic target in HNPCC.
    Original languageEnglish
    JournalCancer Epidemiology, Biomarkers & Prevention
    Volume16
    Issue number9
    Pages (from-to)1760-1766
    Number of pages7
    ISSN1055-9965
    DOIs
    Publication statusPublished - 2007
    MoE publication typeA1 Journal article-refereed

    Fields of Science

    • 311 Basic medicine

    Cite this

    Niemela, Anssi M ; Hynninen, Piritta ; Mecklin, Jukka-Pekka ; Kuopio, Teijo ; Kokko, Antti ; Aaltonen, Lauri A ; Parkkila, Anna-Kaisa ; Pastorekova, Silvia ; Pastorek, Jaromir ; Waheed, Abdul ; Sly, William S ; Ørntoft, Torben F ; Kruhøffer, Mogens ; Haapasalo, Hannu ; Parkkila, Seppo ; Kivelä, Antti. / Carbonic anhydrase IX is highly expressed in hereditary nonpolyposis colorectal cancer. In: Cancer Epidemiology, Biomarkers & Prevention. 2007 ; Vol. 16, No. 9. pp. 1760-1766.
    @article{a9803fd9312142109ab72e452faff938,
    title = "Carbonic anhydrase IX is highly expressed in hereditary nonpolyposis colorectal cancer",
    abstract = "Carbonic anhydrase (CA) II, CA IX, and CA XII are expressed in various neoplasias and have been linked to tumorigenesis. We examined their expression in three different groups of colorectal cancer [i.e., microsatellite stable (MSS), microsatellite instable (MSI), and hereditary nonpolyposis colorectal cancer (HNPCC)]. First, we analyzed gene expression profiles of 113 specimens by a microarray method to study the expression of various CA isozymes in the subgroups of colorectal cancer. The results indicated that mRNAs for CA II and CA XII are down-regulated and CA IX mRNA is up-regulated in all three tumor categories when compared with the normal tissue. The up-regulation of CA IX was greatest in the HNPCC group. For more information, 77 specimens were immunohistochemically stained to study the levels of CA II, CA IX, and CA XII immunohistochemical analyses further confirmed that the subgroups express CA II, CA IX, and CA XII differentially, and the HNPCC tumors express high levels of CA IX Expression of these CAs did not correlate to Dukes stage or grade of differentiation. Our results show that CAs are differentially expressed in the subgroups of colorectal cancer, and CA IX expression seems to be very high in most cases of HNPCC. CA IX could be a potential diagnostic and therapeutic target in HNPCC.",
    keywords = "311 Basic medicine",
    author = "Niemela, {Anssi M} and Piritta Hynninen and Jukka-Pekka Mecklin and Teijo Kuopio and Antti Kokko and Aaltonen, {Lauri A} and Anna-Kaisa Parkkila and Silvia Pastorekova and Jaromir Pastorek and Abdul Waheed and Sly, {William S} and {\O}rntoft, {Torben F} and Mogens Kruh{\o}ffer and Hannu Haapasalo and Seppo Parkkila and Antti Kivel{\"a}",
    year = "2007",
    doi = "10.1158/1055-9965.EPI-07-0080",
    language = "English",
    volume = "16",
    pages = "1760--1766",
    journal = "Cancer Epidemiology, Biomarkers & Prevention",
    issn = "1055-9965",
    publisher = "American Association for Cancer Research",
    number = "9",

    }

    Niemela, AM, Hynninen, P, Mecklin, J-P, Kuopio, T, Kokko, A, Aaltonen, LA, Parkkila, A-K, Pastorekova, S, Pastorek, J, Waheed, A, Sly, WS, Ørntoft, TF, Kruhøffer, M, Haapasalo, H, Parkkila, S & Kivelä, A 2007, 'Carbonic anhydrase IX is highly expressed in hereditary nonpolyposis colorectal cancer', Cancer Epidemiology, Biomarkers & Prevention, vol. 16, no. 9, pp. 1760-1766. https://doi.org/10.1158/1055-9965.EPI-07-0080

    Carbonic anhydrase IX is highly expressed in hereditary nonpolyposis colorectal cancer. / Niemela, Anssi M; Hynninen, Piritta; Mecklin, Jukka-Pekka; Kuopio, Teijo; Kokko, Antti; Aaltonen, Lauri A; Parkkila, Anna-Kaisa; Pastorekova, Silvia; Pastorek, Jaromir; Waheed, Abdul; Sly, William S; Ørntoft, Torben F; Kruhøffer, Mogens; Haapasalo, Hannu; Parkkila, Seppo; Kivelä, Antti.

    In: Cancer Epidemiology, Biomarkers & Prevention, Vol. 16, No. 9, 2007, p. 1760-1766.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Carbonic anhydrase IX is highly expressed in hereditary nonpolyposis colorectal cancer

    AU - Niemela, Anssi M

    AU - Hynninen, Piritta

    AU - Mecklin, Jukka-Pekka

    AU - Kuopio, Teijo

    AU - Kokko, Antti

    AU - Aaltonen, Lauri A

    AU - Parkkila, Anna-Kaisa

    AU - Pastorekova, Silvia

    AU - Pastorek, Jaromir

    AU - Waheed, Abdul

    AU - Sly, William S

    AU - Ørntoft, Torben F

    AU - Kruhøffer, Mogens

    AU - Haapasalo, Hannu

    AU - Parkkila, Seppo

    AU - Kivelä, Antti

    PY - 2007

    Y1 - 2007

    N2 - Carbonic anhydrase (CA) II, CA IX, and CA XII are expressed in various neoplasias and have been linked to tumorigenesis. We examined their expression in three different groups of colorectal cancer [i.e., microsatellite stable (MSS), microsatellite instable (MSI), and hereditary nonpolyposis colorectal cancer (HNPCC)]. First, we analyzed gene expression profiles of 113 specimens by a microarray method to study the expression of various CA isozymes in the subgroups of colorectal cancer. The results indicated that mRNAs for CA II and CA XII are down-regulated and CA IX mRNA is up-regulated in all three tumor categories when compared with the normal tissue. The up-regulation of CA IX was greatest in the HNPCC group. For more information, 77 specimens were immunohistochemically stained to study the levels of CA II, CA IX, and CA XII immunohistochemical analyses further confirmed that the subgroups express CA II, CA IX, and CA XII differentially, and the HNPCC tumors express high levels of CA IX Expression of these CAs did not correlate to Dukes stage or grade of differentiation. Our results show that CAs are differentially expressed in the subgroups of colorectal cancer, and CA IX expression seems to be very high in most cases of HNPCC. CA IX could be a potential diagnostic and therapeutic target in HNPCC.

    AB - Carbonic anhydrase (CA) II, CA IX, and CA XII are expressed in various neoplasias and have been linked to tumorigenesis. We examined their expression in three different groups of colorectal cancer [i.e., microsatellite stable (MSS), microsatellite instable (MSI), and hereditary nonpolyposis colorectal cancer (HNPCC)]. First, we analyzed gene expression profiles of 113 specimens by a microarray method to study the expression of various CA isozymes in the subgroups of colorectal cancer. The results indicated that mRNAs for CA II and CA XII are down-regulated and CA IX mRNA is up-regulated in all three tumor categories when compared with the normal tissue. The up-regulation of CA IX was greatest in the HNPCC group. For more information, 77 specimens were immunohistochemically stained to study the levels of CA II, CA IX, and CA XII immunohistochemical analyses further confirmed that the subgroups express CA II, CA IX, and CA XII differentially, and the HNPCC tumors express high levels of CA IX Expression of these CAs did not correlate to Dukes stage or grade of differentiation. Our results show that CAs are differentially expressed in the subgroups of colorectal cancer, and CA IX expression seems to be very high in most cases of HNPCC. CA IX could be a potential diagnostic and therapeutic target in HNPCC.

    KW - 311 Basic medicine

    U2 - 10.1158/1055-9965.EPI-07-0080

    DO - 10.1158/1055-9965.EPI-07-0080

    M3 - Article

    VL - 16

    SP - 1760

    EP - 1766

    JO - Cancer Epidemiology, Biomarkers & Prevention

    JF - Cancer Epidemiology, Biomarkers & Prevention

    SN - 1055-9965

    IS - 9

    ER -