CENL1 expression in the rib meristem affects stem elongation and the transition to dormancy in populus

Raili Ruonala, Päivi L. H Rinne, Jaakko Kangasjärvi, Christiaan van der Schoot

Research output: Contribution to journalArticleScientificpeer-review

Abstract

We investigated the short day (SD)-induced transition to dormancy in wild-type hybrid poplar (Populus tremula 3 P. tremuloides) and its absence in transgenic poplar overexpressing heterologous PHYTOCHROME A (PHYA). CENTRORADIALIS-LIKE1 (CENL1), a poplar ortholog of Arabidopsis thaliana TERMINAL FLOWER1 (TFL1), was markedly downregulated in the wild-type apex coincident with SD-induced growth cessation. By contrast, poplar overexpressing a heterologous Avena sativa PHYA construct (P35S:AsPHYA), with PHYA accumulating in the rib meristem (RM) and adjacent tissues but not in the shoot apical meristem (SAM), upregulated CENL1 in the RM area coincident with an acceleration of stem elongation. In SD-exposed heterografts, both P35S:AsPHYA and wild-type scions ceased growth and formed buds, whereas only the wild type assumed dormancy and P35S:AsPHYA showed repetitive flushing. This shows that the transition is not dictated by leaf-produced signals but dependent on RM and SAM properties. In view of this, callose-enforced cell isolation in the SAM, associated with suspension of indeterminate growth during dormancy, may require downregulation of CENL1 in the RM. Accordingly, upregulation of CENL1/TFL1 might promote stem elongation in poplar as well as in Arabidopsis during bolting. Together, the results suggest that the RM is particularly sensitive to photoperiodic signals and that CENL1 in the RM influences transition to dormancy in hybrid poplar.
Original languageEnglish
JournalPlant Cell
Volume20
Issue number1
Pages (from-to)59-74
Number of pages16
ISSN1040-4651
DOIs
Publication statusPublished - 2008
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 311 Basic medicine
  • 318 Medical biotechnology
  • 411 Agriculture and forestry
  • 118 Biological sciences
  • 219 Environmental biotechnology
  • 519 Social and economic geography

Cite this

@article{8f76e99454744ac29a8e9437de45e06e,
title = "CENL1 expression in the rib meristem affects stem elongation and the transition to dormancy in populus",
abstract = "We investigated the short day (SD)-induced transition to dormancy in wild-type hybrid poplar (Populus tremula 3 P. tremuloides) and its absence in transgenic poplar overexpressing heterologous PHYTOCHROME A (PHYA). CENTRORADIALIS-LIKE1 (CENL1), a poplar ortholog of Arabidopsis thaliana TERMINAL FLOWER1 (TFL1), was markedly downregulated in the wild-type apex coincident with SD-induced growth cessation. By contrast, poplar overexpressing a heterologous Avena sativa PHYA construct (P35S:AsPHYA), with PHYA accumulating in the rib meristem (RM) and adjacent tissues but not in the shoot apical meristem (SAM), upregulated CENL1 in the RM area coincident with an acceleration of stem elongation. In SD-exposed heterografts, both P35S:AsPHYA and wild-type scions ceased growth and formed buds, whereas only the wild type assumed dormancy and P35S:AsPHYA showed repetitive flushing. This shows that the transition is not dictated by leaf-produced signals but dependent on RM and SAM properties. In view of this, callose-enforced cell isolation in the SAM, associated with suspension of indeterminate growth during dormancy, may require downregulation of CENL1 in the RM. Accordingly, upregulation of CENL1/TFL1 might promote stem elongation in poplar as well as in Arabidopsis during bolting. Together, the results suggest that the RM is particularly sensitive to photoperiodic signals and that CENL1 in the RM influences transition to dormancy in hybrid poplar.",
keywords = "311 Basic medicine, 318 Medical biotechnology, 411 Agriculture and forestry, 118 Biological sciences, 219 Environmental biotechnology, 519 Social and economic geography",
author = "Raili Ruonala and Rinne, {P{\"a}ivi L. H} and Jaakko Kangasj{\"a}rvi and Schoot, {Christiaan van der}",
year = "2008",
doi = "10.1105/tpc.107.056721",
language = "English",
volume = "20",
pages = "59--74",
journal = "Plant Cell",
issn = "1040-4651",
publisher = "American Society of Plant Biologists",
number = "1",

}

CENL1 expression in the rib meristem affects stem elongation and the transition to dormancy in populus. / Ruonala, Raili; Rinne, Päivi L. H; Kangasjärvi, Jaakko; Schoot, Christiaan van der.

In: Plant Cell, Vol. 20, No. 1, 2008, p. 59-74.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - CENL1 expression in the rib meristem affects stem elongation and the transition to dormancy in populus

AU - Ruonala, Raili

AU - Rinne, Päivi L. H

AU - Kangasjärvi, Jaakko

AU - Schoot, Christiaan van der

PY - 2008

Y1 - 2008

N2 - We investigated the short day (SD)-induced transition to dormancy in wild-type hybrid poplar (Populus tremula 3 P. tremuloides) and its absence in transgenic poplar overexpressing heterologous PHYTOCHROME A (PHYA). CENTRORADIALIS-LIKE1 (CENL1), a poplar ortholog of Arabidopsis thaliana TERMINAL FLOWER1 (TFL1), was markedly downregulated in the wild-type apex coincident with SD-induced growth cessation. By contrast, poplar overexpressing a heterologous Avena sativa PHYA construct (P35S:AsPHYA), with PHYA accumulating in the rib meristem (RM) and adjacent tissues but not in the shoot apical meristem (SAM), upregulated CENL1 in the RM area coincident with an acceleration of stem elongation. In SD-exposed heterografts, both P35S:AsPHYA and wild-type scions ceased growth and formed buds, whereas only the wild type assumed dormancy and P35S:AsPHYA showed repetitive flushing. This shows that the transition is not dictated by leaf-produced signals but dependent on RM and SAM properties. In view of this, callose-enforced cell isolation in the SAM, associated with suspension of indeterminate growth during dormancy, may require downregulation of CENL1 in the RM. Accordingly, upregulation of CENL1/TFL1 might promote stem elongation in poplar as well as in Arabidopsis during bolting. Together, the results suggest that the RM is particularly sensitive to photoperiodic signals and that CENL1 in the RM influences transition to dormancy in hybrid poplar.

AB - We investigated the short day (SD)-induced transition to dormancy in wild-type hybrid poplar (Populus tremula 3 P. tremuloides) and its absence in transgenic poplar overexpressing heterologous PHYTOCHROME A (PHYA). CENTRORADIALIS-LIKE1 (CENL1), a poplar ortholog of Arabidopsis thaliana TERMINAL FLOWER1 (TFL1), was markedly downregulated in the wild-type apex coincident with SD-induced growth cessation. By contrast, poplar overexpressing a heterologous Avena sativa PHYA construct (P35S:AsPHYA), with PHYA accumulating in the rib meristem (RM) and adjacent tissues but not in the shoot apical meristem (SAM), upregulated CENL1 in the RM area coincident with an acceleration of stem elongation. In SD-exposed heterografts, both P35S:AsPHYA and wild-type scions ceased growth and formed buds, whereas only the wild type assumed dormancy and P35S:AsPHYA showed repetitive flushing. This shows that the transition is not dictated by leaf-produced signals but dependent on RM and SAM properties. In view of this, callose-enforced cell isolation in the SAM, associated with suspension of indeterminate growth during dormancy, may require downregulation of CENL1 in the RM. Accordingly, upregulation of CENL1/TFL1 might promote stem elongation in poplar as well as in Arabidopsis during bolting. Together, the results suggest that the RM is particularly sensitive to photoperiodic signals and that CENL1 in the RM influences transition to dormancy in hybrid poplar.

KW - 311 Basic medicine

KW - 318 Medical biotechnology

KW - 411 Agriculture and forestry

KW - 118 Biological sciences

KW - 219 Environmental biotechnology

KW - 519 Social and economic geography

U2 - 10.1105/tpc.107.056721

DO - 10.1105/tpc.107.056721

M3 - Article

VL - 20

SP - 59

EP - 74

JO - Plant Cell

JF - Plant Cell

SN - 1040-4651

IS - 1

ER -