Characterization of vB_ApiM_fHyAci03, a novel lytic bacteriophage that infects clinical Acinetobacter strains

Elsi Pulkkinen, Anu Wicklund, Joseph M. O. Oduor, Mikael Skurnik, Saija Kiljunen

Research output: Contribution to journalArticleScientificpeer-review

Abstract

We present here the isolation and characterization of Acinetobacter pittii phage vB_ApiM_fHyAci03 (fHyAci03), which belongs to the family Myoviridae. The fHyAci03 genome was found to be 165,975 bp in length and predicted to contain 255 genes. While the whole genome was 92.4% identical to Acinetobacter baumannii phage KARL-1, phylogenetic analysis based on phage long distal tail fiber amino acid sequences assigned fHyAci03 and KARL-1 to different subclusters, reflecting their different host species. Together with phylogenetic analysis, genome comparisons indicated that fHyAci03 is a novel member of the subfamily Tevenvirinae. Host range experiments revealed that fHyAci03 could infect two clinical strains of Acinetobacter nosocomialis and six clinical strains of A. pittii. Thus, fHyAci03 is a novel lytic phage that infects clinical Acinetobacter strains and represents a potential new candidate to be used in phage therapy.
Original languageEnglish
JournalArchives of Virology
Volume164
Issue number8
Pages (from-to)2197-2199
Number of pages3
ISSN1432-8798
DOIs
Publication statusPublished - Aug 2019
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1182 Biochemistry, cell and molecular biology
  • 3111 Biomedicine

Cite this

@article{a2d39fcd341c41a697963bb701bfdfed,
title = "Characterization of vB_ApiM_fHyAci03, a novel lytic bacteriophage that infects clinical Acinetobacter strains",
abstract = "We present here the isolation and characterization of Acinetobacter pittii phage vB_ApiM_fHyAci03 (fHyAci03), which belongs to the family Myoviridae. The fHyAci03 genome was found to be 165,975 bp in length and predicted to contain 255 genes. While the whole genome was 92.4{\%} identical to Acinetobacter baumannii phage KARL-1, phylogenetic analysis based on phage long distal tail fiber amino acid sequences assigned fHyAci03 and KARL-1 to different subclusters, reflecting their different host species. Together with phylogenetic analysis, genome comparisons indicated that fHyAci03 is a novel member of the subfamily Tevenvirinae. Host range experiments revealed that fHyAci03 could infect two clinical strains of Acinetobacter nosocomialis and six clinical strains of A. pittii. Thus, fHyAci03 is a novel lytic phage that infects clinical Acinetobacter strains and represents a potential new candidate to be used in phage therapy.",
keywords = "1182 Biochemistry, cell and molecular biology, 3111 Biomedicine",
author = "Elsi Pulkkinen and Anu Wicklund and Oduor, {Joseph M. O.} and Mikael Skurnik and Saija Kiljunen",
year = "2019",
month = "8",
doi = "10.1007/s00705-019-04284-z",
language = "English",
volume = "164",
pages = "2197--2199",
journal = "Archives of Virology",
issn = "0304-8608",
publisher = "SPRINGER WIEN",
number = "8",

}

Characterization of vB_ApiM_fHyAci03, a novel lytic bacteriophage that infects clinical Acinetobacter strains. / Pulkkinen, Elsi; Wicklund, Anu; Oduor, Joseph M. O.; Skurnik, Mikael; Kiljunen, Saija.

In: Archives of Virology, Vol. 164, No. 8, 08.2019, p. 2197-2199.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Characterization of vB_ApiM_fHyAci03, a novel lytic bacteriophage that infects clinical Acinetobacter strains

AU - Pulkkinen, Elsi

AU - Wicklund, Anu

AU - Oduor, Joseph M. O.

AU - Skurnik, Mikael

AU - Kiljunen, Saija

PY - 2019/8

Y1 - 2019/8

N2 - We present here the isolation and characterization of Acinetobacter pittii phage vB_ApiM_fHyAci03 (fHyAci03), which belongs to the family Myoviridae. The fHyAci03 genome was found to be 165,975 bp in length and predicted to contain 255 genes. While the whole genome was 92.4% identical to Acinetobacter baumannii phage KARL-1, phylogenetic analysis based on phage long distal tail fiber amino acid sequences assigned fHyAci03 and KARL-1 to different subclusters, reflecting their different host species. Together with phylogenetic analysis, genome comparisons indicated that fHyAci03 is a novel member of the subfamily Tevenvirinae. Host range experiments revealed that fHyAci03 could infect two clinical strains of Acinetobacter nosocomialis and six clinical strains of A. pittii. Thus, fHyAci03 is a novel lytic phage that infects clinical Acinetobacter strains and represents a potential new candidate to be used in phage therapy.

AB - We present here the isolation and characterization of Acinetobacter pittii phage vB_ApiM_fHyAci03 (fHyAci03), which belongs to the family Myoviridae. The fHyAci03 genome was found to be 165,975 bp in length and predicted to contain 255 genes. While the whole genome was 92.4% identical to Acinetobacter baumannii phage KARL-1, phylogenetic analysis based on phage long distal tail fiber amino acid sequences assigned fHyAci03 and KARL-1 to different subclusters, reflecting their different host species. Together with phylogenetic analysis, genome comparisons indicated that fHyAci03 is a novel member of the subfamily Tevenvirinae. Host range experiments revealed that fHyAci03 could infect two clinical strains of Acinetobacter nosocomialis and six clinical strains of A. pittii. Thus, fHyAci03 is a novel lytic phage that infects clinical Acinetobacter strains and represents a potential new candidate to be used in phage therapy.

KW - 1182 Biochemistry, cell and molecular biology

KW - 3111 Biomedicine

U2 - 10.1007/s00705-019-04284-z

DO - 10.1007/s00705-019-04284-z

M3 - Article

VL - 164

SP - 2197

EP - 2199

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 8

ER -