Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing

Cedric Woudstra; Tommi Mäklin; Yagmur Derman; Luca Bano; Hanna  Skarin; Christelle  Mazuet; Antti Honkela; Miia Lindström

doi:10.1128/MRA.01364-20

Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing

Cedric Woudstra, Tommi Mäklin, Yagmur Derman, Luca Bano, Hanna Skarin, Christelle Mazuet, Antti Honkela, Miia Lindström

Research output: Contribution to journal › Article › Scientific › peer-review

Abstract

Clostridium botulinum group III is the anaerobic Gram-positive bacterium producing the deadly neurotoxin responsible for animal botulism. Here, we used long-read sequencing to produce four complete genomes from Clostridium botulinum group III neurotoxin types C, D, C/D, and D/C. The protocol for obtaining high-molecular-weight DNA from C. botulinum group III is described.

Original language	English
Article number	e01364-20
Journal	Microbiology Resource Announcements
Volume	10
Issue number	22
Number of pages	4
ISSN	2576-098X
DOIs	https://doi.org/10.1128/MRA.01364-20
Publication status	Published - Jun 2021
MoE publication type	A1 Journal article-refereed

Fields of Science

11832 Microbiology and virology
113 Computer and information sciences
PHAGE

Access to Document

10.1128/MRA.01364-20Licence: CC BY

MRA.01364-20Final published version, 523 KBLicence: CC BY

Cite this

@article{049eb37b5258433084f72dc6655ff934,

title = "Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing",

abstract = "Clostridium botulinum group III is the anaerobic Gram-positive bacterium producing the deadly neurotoxin responsible for animal botulism. Here, we used long-read sequencing to produce four complete genomes from Clostridium botulinum group III neurotoxin types C, D, C/D, and D/C. The protocol for obtaining high-molecular-weight DNA from C. botulinum group III is described.",

keywords = "11832 Microbiology and virology, 113 Computer and information sciences, PHAGE",

author = "Cedric Woudstra and Tommi M{\"a}klin and Yagmur Derman and Luca Bano and Hanna Skarin and Christelle Mazuet and Antti Honkela and Miia Lindstr{\"o}m",

year = "2021",

month = jun,

doi = "10.1128/MRA.01364-20",

language = "English",

volume = "10",

journal = "Microbiology Resource Announcements",

issn = "2576-098X",

publisher = "American Society for Microbiology",

number = "22",

}

TY - JOUR

T1 - Closing Clostridium botulinum Group III Genomes Using Long-Read Sequencing

AU - Woudstra, Cedric

AU - Mäklin, Tommi

AU - Derman, Yagmur

AU - Bano, Luca

AU - Skarin, Hanna

AU - Mazuet, Christelle

AU - Honkela, Antti

AU - Lindström, Miia

PY - 2021/6

Y1 - 2021/6

N2 - Clostridium botulinum group III is the anaerobic Gram-positive bacterium producing the deadly neurotoxin responsible for animal botulism. Here, we used long-read sequencing to produce four complete genomes from Clostridium botulinum group III neurotoxin types C, D, C/D, and D/C. The protocol for obtaining high-molecular-weight DNA from C. botulinum group III is described.

AB - Clostridium botulinum group III is the anaerobic Gram-positive bacterium producing the deadly neurotoxin responsible for animal botulism. Here, we used long-read sequencing to produce four complete genomes from Clostridium botulinum group III neurotoxin types C, D, C/D, and D/C. The protocol for obtaining high-molecular-weight DNA from C. botulinum group III is described.

KW - 11832 Microbiology and virology

KW - 113 Computer and information sciences

KW - PHAGE

U2 - 10.1128/MRA.01364-20

DO - 10.1128/MRA.01364-20

M3 - Article

SN - 2576-098X

VL - 10

JO - Microbiology Resource Announcements

JF - Microbiology Resource Announcements

IS - 22

M1 - e01364-20

ER -