Co-expression of Nisin Z and Leucocin C as a Basis for Effective Protection Against Listeria monocytogenes in Pasteurized Milk

Y Fu, W Qiao, D Zhu, X Wang, F Liu, H Xu, Per Erik Joakim Saris, Osacar Kuipers, Mingqiang Qiao

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Nisin, an important bacteriocin from Lactococcus lactis subsp., is primarily active against various Gram-positive bacteria. Leucocin C, produced by Leuconostoc carnosum 4010, is a class IIa bacteriocin used to inhibit the growth of Listeria monocytogenes. Because two bacteriocins have different modes of action, the combined use of them could be a potential strategy for effective inhibition of foodborne pathogens. In this study, L. lactis N8-r-lecCI (N8 harboring lecCI gene) coexpressing nisin–leucocin C was constructed based on the food-grade carrier L. lactis N8. Production of both bacteriocins was stably maintained. Antimicrobial measurements showed that the recombinant strain is effectively against Listeria monocytogenes and Staphylococcus aureus and moderately against Salmonella enterica serovar Enteritidis and Escherichia coli because of its stronger antibacterial activity than the parental strain, this result first demonstrated that the co-expression of nisin and leucocin C results in highly efficient antimicrobial activity. The checkerboard assay showed that the antibacterial activity of L. lactis N8-r-lecCI supernatant was enhanced in the presence of low concentration of EDTA. Analysis of the scanning electron microscope image showed the biggest cellular morphology change in L. monocytogenes treated with a mixture of EDTA and L. lactis N8-r-lecCI supernatant. The practical effect was verified in pasteurized milk through time-kill assay. The L. lactis N8-r-lecCI strain expressing both nisin and leucocin C has a promising application prospect in pasteurized milk processing and preservation because of its strong antibacterial activity.
Original languageEnglish
Article number547
JournalFrontiers in Microbiology
Volume2018 / 9
Pages (from-to)1 - 11
Number of pages11
ISSN1664-302X
DOIs
Publication statusPublished - 23 Mar 2018
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 416 Food Science
  • 1183 Plant biology, microbiology, virology
  • nisin
  • Leucocin C
  • Lactococcus lactis
  • Lactic acid bacteria
  • food
  • safety
  • risk assessment
  • Escherichia coli

Cite this

Fu, Y ; Qiao, W ; Zhu, D ; Wang, X ; Liu, F ; Xu, H ; Saris, Per Erik Joakim ; Kuipers, Osacar ; Qiao, Mingqiang. / Co-expression of Nisin Z and Leucocin C as a Basis for Effective Protection Against Listeria monocytogenes in Pasteurized Milk. In: Frontiers in Microbiology. 2018 ; Vol. 2018 / 9 . pp. 1 - 11.
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title = "Co-expression of Nisin Z and Leucocin C as a Basis for Effective Protection Against Listeria monocytogenes in Pasteurized Milk",
abstract = "Nisin, an important bacteriocin from Lactococcus lactis subsp., is primarily active against various Gram-positive bacteria. Leucocin C, produced by Leuconostoc carnosum 4010, is a class IIa bacteriocin used to inhibit the growth of Listeria monocytogenes. Because two bacteriocins have different modes of action, the combined use of them could be a potential strategy for effective inhibition of foodborne pathogens. In this study, L. lactis N8-r-lecCI (N8 harboring lecCI gene) coexpressing nisin–leucocin C was constructed based on the food-grade carrier L. lactis N8. Production of both bacteriocins was stably maintained. Antimicrobial measurements showed that the recombinant strain is effectively against Listeria monocytogenes and Staphylococcus aureus and moderately against Salmonella enterica serovar Enteritidis and Escherichia coli because of its stronger antibacterial activity than the parental strain, this result first demonstrated that the co-expression of nisin and leucocin C results in highly efficient antimicrobial activity. The checkerboard assay showed that the antibacterial activity of L. lactis N8-r-lecCI supernatant was enhanced in the presence of low concentration of EDTA. Analysis of the scanning electron microscope image showed the biggest cellular morphology change in L. monocytogenes treated with a mixture of EDTA and L. lactis N8-r-lecCI supernatant. The practical effect was verified in pasteurized milk through time-kill assay. The L. lactis N8-r-lecCI strain expressing both nisin and leucocin C has a promising application prospect in pasteurized milk processing and preservation because of its strong antibacterial activity.",
keywords = "416 Food Science, 1183 Plant biology, microbiology, virology, nisin, Leucocin C, Lactococcus lactis, Lactic acid bacteria, food, safety, risk assessment, Escherichia coli",
author = "Y Fu and W Qiao and D Zhu and X Wang and F Liu and H Xu and Saris, {Per Erik Joakim} and Osacar Kuipers and Mingqiang Qiao",
year = "2018",
month = "3",
day = "23",
doi = "10.3389/fmicb.2018.00547",
language = "English",
volume = "2018 / 9",
pages = "1 -- 11",
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Co-expression of Nisin Z and Leucocin C as a Basis for Effective Protection Against Listeria monocytogenes in Pasteurized Milk. / Fu, Y; Qiao, W; Zhu, D; Wang, X; Liu, F; Xu, H; Saris, Per Erik Joakim; Kuipers, Osacar; Qiao, Mingqiang.

In: Frontiers in Microbiology, Vol. 2018 / 9 , 547, 23.03.2018, p. 1 - 11.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Co-expression of Nisin Z and Leucocin C as a Basis for Effective Protection Against Listeria monocytogenes in Pasteurized Milk

AU - Fu, Y

AU - Qiao, W

AU - Zhu, D

AU - Wang, X

AU - Liu, F

AU - Xu, H

AU - Saris, Per Erik Joakim

AU - Kuipers, Osacar

AU - Qiao, Mingqiang

PY - 2018/3/23

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N2 - Nisin, an important bacteriocin from Lactococcus lactis subsp., is primarily active against various Gram-positive bacteria. Leucocin C, produced by Leuconostoc carnosum 4010, is a class IIa bacteriocin used to inhibit the growth of Listeria monocytogenes. Because two bacteriocins have different modes of action, the combined use of them could be a potential strategy for effective inhibition of foodborne pathogens. In this study, L. lactis N8-r-lecCI (N8 harboring lecCI gene) coexpressing nisin–leucocin C was constructed based on the food-grade carrier L. lactis N8. Production of both bacteriocins was stably maintained. Antimicrobial measurements showed that the recombinant strain is effectively against Listeria monocytogenes and Staphylococcus aureus and moderately against Salmonella enterica serovar Enteritidis and Escherichia coli because of its stronger antibacterial activity than the parental strain, this result first demonstrated that the co-expression of nisin and leucocin C results in highly efficient antimicrobial activity. The checkerboard assay showed that the antibacterial activity of L. lactis N8-r-lecCI supernatant was enhanced in the presence of low concentration of EDTA. Analysis of the scanning electron microscope image showed the biggest cellular morphology change in L. monocytogenes treated with a mixture of EDTA and L. lactis N8-r-lecCI supernatant. The practical effect was verified in pasteurized milk through time-kill assay. The L. lactis N8-r-lecCI strain expressing both nisin and leucocin C has a promising application prospect in pasteurized milk processing and preservation because of its strong antibacterial activity.

AB - Nisin, an important bacteriocin from Lactococcus lactis subsp., is primarily active against various Gram-positive bacteria. Leucocin C, produced by Leuconostoc carnosum 4010, is a class IIa bacteriocin used to inhibit the growth of Listeria monocytogenes. Because two bacteriocins have different modes of action, the combined use of them could be a potential strategy for effective inhibition of foodborne pathogens. In this study, L. lactis N8-r-lecCI (N8 harboring lecCI gene) coexpressing nisin–leucocin C was constructed based on the food-grade carrier L. lactis N8. Production of both bacteriocins was stably maintained. Antimicrobial measurements showed that the recombinant strain is effectively against Listeria monocytogenes and Staphylococcus aureus and moderately against Salmonella enterica serovar Enteritidis and Escherichia coli because of its stronger antibacterial activity than the parental strain, this result first demonstrated that the co-expression of nisin and leucocin C results in highly efficient antimicrobial activity. The checkerboard assay showed that the antibacterial activity of L. lactis N8-r-lecCI supernatant was enhanced in the presence of low concentration of EDTA. Analysis of the scanning electron microscope image showed the biggest cellular morphology change in L. monocytogenes treated with a mixture of EDTA and L. lactis N8-r-lecCI supernatant. The practical effect was verified in pasteurized milk through time-kill assay. The L. lactis N8-r-lecCI strain expressing both nisin and leucocin C has a promising application prospect in pasteurized milk processing and preservation because of its strong antibacterial activity.

KW - 416 Food Science

KW - 1183 Plant biology, microbiology, virology

KW - nisin

KW - Leucocin C

KW - Lactococcus lactis

KW - Lactic acid bacteria

KW - food

KW - safety

KW - risk assessment

KW - Escherichia coli

U2 - 10.3389/fmicb.2018.00547

DO - 10.3389/fmicb.2018.00547

M3 - Article

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JO - Frontiers in Microbiology

JF - Frontiers in Microbiology

SN - 1664-302X

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ER -