Comparative analysis of enzyme production patterns of lignocellulose degradation of two white rot fungi: Obba rivulosa and Gelatoporia subvermispora

Mila Marinovic, Marcos Di Falco, Maria Victoria Aguilar Pontes, Andras Gorzsas, Adrian Tsang , Ronald de Vries, Miia Mäkelä, Kristiina Hilden

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The unique ability of basidiomycete white rot fungi to degrade all components of plant cell walls makes them indispensable organisms in the global carbon cycle. In this study, we analyzed the proteomes of two closely related white rot fungi, Obba rivulosa and Gelatoporia subvermispora, during eight-week cultivation on solid spruce wood. Plant cell wall degrading carbohydrate-active enzymes (CAZymes) represented approximately 5% of the total proteins in both species. A core set of orthologous plant cell wall degrading CAZymes was shared between these species on spruce suggesting a conserved plant biomass degradation approach in this clade of basidiomycete fungi. However, differences in time-dependent production of plant cell wall degrading enzymes may be due to differences among initial growth rates of these species on solid spruce wood. The obtained results provide insight into specific enzymes and enzyme sets that are produced during the degradation of solid spruce wood in these fungi. These findings expand the knowledge on enzyme production in nature-mimicking conditions and may contribute to the exploitation of white rot fungi and their enzymes for biotechnological applications.

Original languageEnglish
Article number1017
JournalBiomolecules
Volume12
Issue number8
Number of pages14
ISSN2218-273X
DOIs
Publication statusPublished - 22 Jul 2022
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 11832 Microbiology and virology
  • Obba rivulosa
  • Gelatoporia subvermispora
  • proteome
  • CAZymes
  • lignin biodegradation
  • LC-MS/MS
  • white rot fungi
  • CERIPORIOPSIS-SUBVERMISPORA
  • PHANEROCHAETE-CHRYSOSPORIUM
  • MANGANESE PEROXIDASES
  • COMPARATIVE GENOMICS
  • DICHOMITUS-SQUALENS
  • GENE-EXPRESSION
  • PLANT BIOMASS
  • OXALIC-ACID
  • WOOD-DECAY
  • BIODEGRADATION

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