Comparative Genome Analysis and Spore heat Resistance Assay Reveal a New Component to Population Structure and Genome Epidemiology Within Clostridium perfringens Enterotoxin-Carrying Isolates

Kaisa Jaakkola, Kira Virtanen, Päivi Lahti, Riikka Keto-Timonen, Miia Lindström, Hannu Korkeala

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Clostridium perfringens causes a variety of human and animal enteric diseases including food poisoning, antibiotic-associated diarrhea, and necrotic enteritis. Yet, the reservoirs of enteropathogenic enterotoxin-producing strains remain unknown. We conducted a genomic comparison of 290 strains and a heat resistance phenotyping of 30 C. perfringens strains to elucidate the population structure and ecology of this pathogen. C. perfringens genomes shared a conserved genetic backbone with more than half of the genes of an average genome conserved in >95% of strains. The cpe-carrying isolates were found to share genetic context: the cpe-carrying plasmids had different distribution patterns within the genetic lineages and the estimated pan genome of cpe-carrying isolates had a larger core genome and a smaller accessory genome compared to that of 290 strains. We characterize cpe-negative strains related to chromosomal cpe-carrying strains elucidating the origin of these strains and disclose two distinct groups of chromosomal cpe-carrying strains with different virulence characteristics, spore heat resistance properties, and, presumably, ecological niche. Finally, an antibiotic-associated diarrhea isolate carrying two copies of the enterotoxin cpe gene and the associated genetic lineage with the potential for the emergence of similar strains are outlined. With C. perfringens as an example, implications of input genome quality for pan genome analysis are discussed. Our study furthers the understanding of genome epidemiology and population structure of enteropathogenic C. perfringens and brings new insight into this important pathogen and its reservoirs.
Original languageEnglish
Article number717176
JournalFrontiers in Microbiology
Volume12
Number of pages16
ISSN1664-302X
DOIs
Publication statusPublished - 8 Sep 2021
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 11832 Microbiology and virology
  • 416 Food Science
  • 413 Veterinary science
  • Clostridium perfringens
  • core genome
  • enterotoxin (CPE)
  • spore heat resistance
  • gastrointestinal disease
  • comparative genome analysis

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