Comparison of Clostridium botulinum genomes shows the absence of cold shock protein coding genes in type E neurotoxin producing strains

Research output: Contribution to journalArticleScientificpeer-review

Abstract

To collect specific information about the genetic mechanisms that Clostridium botulinum strains utilise when adapting to changing environments, 16 C. botulinum genomes were analysed with comparative genome sequence analysis. Particular attention was paid to low temperature adaptation and the presence of cold shock protein coding genes in these genomes was evaluated. Surprisingly, unlike any other studied strains, the type E neurotoxin-producing strains lacked these extremely conserved genes. This finding suggests unique mechanisms for the cold tolerance of these strains and offers a new perspective into the investigations concerning this subject. The sizes of the pangenome and core genome of a certain bacterial species are considered to reflect the versatility of the species. While the pangenome of C. botulinum was very large, the core genome appeared strikingly small, both findings highlighting the great diversity of C. botulinum strains.

Original languageEnglish
JournalBotulinum Journal
Volume2
Issue number3-4
Pages (from-to)189-207
Number of pages19
ISSN1754-7318
DOIs
Publication statusPublished - 2013
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 413 Veterinary science
  • CLOSTRIDIUM BOTULINUM
  • NEUROTOXIN
  • PANGENOME
  • CORE GENOME
  • GENOMIC COMPARISON
  • COMPARATIVE GENOMICS
  • FUNCTIONAL STUDIES
  • ORTHOLOGOUS GENES
  • COLD SHOCK PROTEIN
  • CSP
  • COLD TOLERANCE MECHANISM
  • FOOD SAFETY
  • 1183 Plant biology, microbiology, virology
  • 3111 Biomedicine

Cite this

@article{7e61951cbdd744aabf7ba7bd128fb946,
title = "Comparison of Clostridium botulinum genomes shows the absence of cold shock protein coding genes in type E neurotoxin producing strains",
abstract = "To collect specific information about the genetic mechanisms that Clostridium botulinum strains utilise when adapting to changing environments, 16 C. botulinum genomes were analysed with comparative genome sequence analysis. Particular attention was paid to low temperature adaptation and the presence of cold shock protein coding genes in these genomes was evaluated. Surprisingly, unlike any other studied strains, the type E neurotoxin-producing strains lacked these extremely conserved genes. This finding suggests unique mechanisms for the cold tolerance of these strains and offers a new perspective into the investigations concerning this subject. The sizes of the pangenome and core genome of a certain bacterial species are considered to reflect the versatility of the species. While the pangenome of C. botulinum was very large, the core genome appeared strikingly small, both findings highlighting the great diversity of C. botulinum strains.",
keywords = "413 Veterinary science, CLOSTRIDIUM BOTULINUM, NEUROTOXIN, PANGENOME, CORE GENOME, GENOMIC COMPARISON, COMPARATIVE GENOMICS, FUNCTIONAL STUDIES, ORTHOLOGOUS GENES, COLD SHOCK PROTEIN, CSP, COLD TOLERANCE MECHANISM, FOOD SAFETY, 1183 Plant biology, microbiology, virology, 3111 Biomedicine",
author = "Henna S{\"o}derholm and Kaisa Jaakkola and Panu Somervuo and Pia Laine and Petri Auvinen and Lars Paulin and Miia Lindstr{\"o}m and Hannu Korkeala",
year = "2013",
doi = "10.1504/TBJ.2013.055662",
language = "English",
volume = "2",
pages = "189--207",
journal = "Botulinum Journal",
issn = "1754-7318",
publisher = "INDERSCIENCE PUBLISHERS",
number = "3-4",

}

TY - JOUR

T1 - Comparison of Clostridium botulinum genomes shows the absence of cold shock protein coding genes in type E neurotoxin producing strains

AU - Söderholm, Henna

AU - Jaakkola, Kaisa

AU - Somervuo, Panu

AU - Laine, Pia

AU - Auvinen, Petri

AU - Paulin, Lars

AU - Lindström, Miia

AU - Korkeala, Hannu

PY - 2013

Y1 - 2013

N2 - To collect specific information about the genetic mechanisms that Clostridium botulinum strains utilise when adapting to changing environments, 16 C. botulinum genomes were analysed with comparative genome sequence analysis. Particular attention was paid to low temperature adaptation and the presence of cold shock protein coding genes in these genomes was evaluated. Surprisingly, unlike any other studied strains, the type E neurotoxin-producing strains lacked these extremely conserved genes. This finding suggests unique mechanisms for the cold tolerance of these strains and offers a new perspective into the investigations concerning this subject. The sizes of the pangenome and core genome of a certain bacterial species are considered to reflect the versatility of the species. While the pangenome of C. botulinum was very large, the core genome appeared strikingly small, both findings highlighting the great diversity of C. botulinum strains.

AB - To collect specific information about the genetic mechanisms that Clostridium botulinum strains utilise when adapting to changing environments, 16 C. botulinum genomes were analysed with comparative genome sequence analysis. Particular attention was paid to low temperature adaptation and the presence of cold shock protein coding genes in these genomes was evaluated. Surprisingly, unlike any other studied strains, the type E neurotoxin-producing strains lacked these extremely conserved genes. This finding suggests unique mechanisms for the cold tolerance of these strains and offers a new perspective into the investigations concerning this subject. The sizes of the pangenome and core genome of a certain bacterial species are considered to reflect the versatility of the species. While the pangenome of C. botulinum was very large, the core genome appeared strikingly small, both findings highlighting the great diversity of C. botulinum strains.

KW - 413 Veterinary science

KW - CLOSTRIDIUM BOTULINUM

KW - NEUROTOXIN

KW - PANGENOME

KW - CORE GENOME

KW - GENOMIC COMPARISON

KW - COMPARATIVE GENOMICS

KW - FUNCTIONAL STUDIES

KW - ORTHOLOGOUS GENES

KW - COLD SHOCK PROTEIN

KW - CSP

KW - COLD TOLERANCE MECHANISM

KW - FOOD SAFETY

KW - 1183 Plant biology, microbiology, virology

KW - 3111 Biomedicine

U2 - 10.1504/TBJ.2013.055662

DO - 10.1504/TBJ.2013.055662

M3 - Article

VL - 2

SP - 189

EP - 207

JO - Botulinum Journal

JF - Botulinum Journal

SN - 1754-7318

IS - 3-4

ER -