Complete Genomic Sequence of the Lytic Bacteriophage φYeO3-12 of Yersinia enterocolitica Serotype O:3

Research output: Contribution to journalArticleScientificpeer-review

Abstract

φYeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3. The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6%, and 54 putative genes, which are all transcribed from the same DNA strand. Functions were assigned to 30 genes based on the similarity of the predicted products to known proteins. A striking feature of the φYeO3-12 genome is its extensive similarity to the coliphage T3 and T7 genomes; most of the predicted φYeO3-12 gene products were >70% identical to those of T3, and the overall organizations of the genomes were similar. In addition to an identical promoter specificity, φYeO3-12 shares several common features with T3, nonsubjectibility to F exclusion and growth on Shigella sonneiD2371-48 (M. Pajunen, S. Kiljunen, and M. Skurnik, J. Bacteriol. 182:5114–5120, 2000). These findings indicate that φYeO3-12 is a T3-like phage that has adapted to Y. enterocolitica O:3 or vice versa. This is the first dsDNA yersiniophage genome sequence to be reported.
Original languageEnglish
JournalJournal of Bacteriology
Volume183
Issue number6
Pages (from-to)1928-1937
Number of pages10
ISSN0021-9193
DOIs
Publication statusPublished - 2001
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1183 Plant biology, microbiology, virology

Cite this

@article{86de80c7e6554d029a6b2cbcf2e82008,
title = "Complete Genomic Sequence of the Lytic Bacteriophage φYeO3-12 of Yersinia enterocolitica Serotype O:3",
abstract = "φYeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3. The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6{\%}, and 54 putative genes, which are all transcribed from the same DNA strand. Functions were assigned to 30 genes based on the similarity of the predicted products to known proteins. A striking feature of the φYeO3-12 genome is its extensive similarity to the coliphage T3 and T7 genomes; most of the predicted φYeO3-12 gene products were >70{\%} identical to those of T3, and the overall organizations of the genomes were similar. In addition to an identical promoter specificity, φYeO3-12 shares several common features with T3, nonsubjectibility to F exclusion and growth on Shigella sonneiD2371-48 (M. Pajunen, S. Kiljunen, and M. Skurnik, J. Bacteriol. 182:5114–5120, 2000). These findings indicate that φYeO3-12 is a T3-like phage that has adapted to Y. enterocolitica O:3 or vice versa. This is the first dsDNA yersiniophage genome sequence to be reported.",
keywords = "1183 Plant biology, microbiology, virology",
author = "Pajunen, {Maria I.} and Kiljunen, {Saija J.} and S{\"o}derholm, {M. E.-Lotta} and Mikael Skurnik",
year = "2001",
doi = "10.1128/JB.183.6.1928-1937.2001",
language = "English",
volume = "183",
pages = "1928--1937",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "6",

}

Complete Genomic Sequence of the Lytic Bacteriophage φYeO3-12 of Yersinia enterocolitica Serotype O:3. / Pajunen, Maria I.; Kiljunen, Saija J.; Söderholm, M. E.-Lotta; Skurnik, Mikael.

In: Journal of Bacteriology, Vol. 183, No. 6, 2001, p. 1928-1937.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Complete Genomic Sequence of the Lytic Bacteriophage φYeO3-12 of Yersinia enterocolitica Serotype O:3

AU - Pajunen, Maria I.

AU - Kiljunen, Saija J.

AU - Söderholm, M. E.-Lotta

AU - Skurnik, Mikael

PY - 2001

Y1 - 2001

N2 - φYeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3. The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6%, and 54 putative genes, which are all transcribed from the same DNA strand. Functions were assigned to 30 genes based on the similarity of the predicted products to known proteins. A striking feature of the φYeO3-12 genome is its extensive similarity to the coliphage T3 and T7 genomes; most of the predicted φYeO3-12 gene products were >70% identical to those of T3, and the overall organizations of the genomes were similar. In addition to an identical promoter specificity, φYeO3-12 shares several common features with T3, nonsubjectibility to F exclusion and growth on Shigella sonneiD2371-48 (M. Pajunen, S. Kiljunen, and M. Skurnik, J. Bacteriol. 182:5114–5120, 2000). These findings indicate that φYeO3-12 is a T3-like phage that has adapted to Y. enterocolitica O:3 or vice versa. This is the first dsDNA yersiniophage genome sequence to be reported.

AB - φYeO3-12 is a T3-related lytic bacteriophage of Yersinia enterocolitica serotype O:3. The nucleotide sequence of the 39,600-bp linear double-stranded DNA (dsDNA) genome was determined. The phage genome has direct terminal repeats of 232 bp, a GC content of 50.6%, and 54 putative genes, which are all transcribed from the same DNA strand. Functions were assigned to 30 genes based on the similarity of the predicted products to known proteins. A striking feature of the φYeO3-12 genome is its extensive similarity to the coliphage T3 and T7 genomes; most of the predicted φYeO3-12 gene products were >70% identical to those of T3, and the overall organizations of the genomes were similar. In addition to an identical promoter specificity, φYeO3-12 shares several common features with T3, nonsubjectibility to F exclusion and growth on Shigella sonneiD2371-48 (M. Pajunen, S. Kiljunen, and M. Skurnik, J. Bacteriol. 182:5114–5120, 2000). These findings indicate that φYeO3-12 is a T3-like phage that has adapted to Y. enterocolitica O:3 or vice versa. This is the first dsDNA yersiniophage genome sequence to be reported.

KW - 1183 Plant biology, microbiology, virology

U2 - 10.1128/JB.183.6.1928-1937.2001

DO - 10.1128/JB.183.6.1928-1937.2001

M3 - Article

VL - 183

SP - 1928

EP - 1937

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 6

ER -