Construction and characterization of synthetic bacterial community for experimental ecology and evolution

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Experimental microbial ecology and evolution have yielded foundational insights into ecological and evolutionary processes using simple microcosm setups and phenotypic assays with one- or two-species model systems. The fields are now increasingly incorporating more complex systems and exploration of the molecular basis of observations. For this purpose, simplified, manageable and well-defined multispecies model systems are required that can be easily investigated using culturing and high-throughput sequencing approaches, bridging the gap between simpler and more complex synthetic or natural systems. Here we address this need by constructing a completely synthetic 33 bacterial strain community that can be cultured in simple laboratory conditions. We provide whole-genome data for all the strains as well as metadata about genomic features and phenotypic traits that allow resolving individual strains by amplicon sequencing and facilitate a variety of envisioned mechanistic studies. We further show that a large proportion of the strains exhibit coexistence in co-culture over serial transfer for 48 days in the absence of any experimental manipulation to maintain diversity. The constructed bacterial community can be a valuable resource in future experimental work.

Original languageEnglish
Article number312
JournalFrontiers in Genetics
Volume9
Number of pages12
ISSN1664-8021
DOIs
Publication statusPublished - 14 Aug 2018
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1181 Ecology, evolutionary biology
  • microbial community
  • model system
  • synthetic ecology
  • experimental evolution
  • whole-genome sequencing
  • MICROCOSM EXPERIMENTS
  • SPECIES INTERACTIONS
  • LIMITED RELEVANCE
  • ECOSYSTEM ECOLOGY
  • ESCHERICHIA-COLI
  • READ ALIGNMENT
  • SEARCH TOOL
  • IN-SITU
  • SYSTEM
  • COOPERATION

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