Continuous nisin production with bioengineered Lactococcus lactis strains

O. Simsek, N. Akkoc, A. H. Con, F. Ozcelik, P. E. J. Saris, Mustafa Akcelik

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Nisin production in continuous cultures of bioengineered Lactococcus lactis strains that incorporate additional immunity and regulation genes was studied. Highest nisin activities were observed at 0.2 h(-1) dilution rate and 12.5 g l(-1) fructose concentration for all strains. Recombinant strains were able to produce greater amounts of nisin at dilution rates below 0.3 h(-1) compared to the control strain. However, this significant difference disappeared at dilution rates of 0.4 and 0.5 h(-1). For the strains LL27, LAC338, LAC339, and LAC340, optimum conditions for nisin production were determined to be at 0.29, 0.26, 0.27, and 0.27 h(-1) dilution rates and 11.95, 12.01, 11.63, and 12.50 g l(-1) fructose concentrations, respectively. The highest nisin productivity, 496 IU ml(-1) h(-1), was achieved with LAC339. The results of this study suggest that low dilution rates stabilize the high specific nisin productivity of the bioengineered strains in continuous fermentation. Moreover, response surface methodology analysis showed that regulation genes yielded high nisin productivity at wide ranges of dilution rates and fructose concentrations.
Original languageEnglish
JournalJournal of Industrial Microbiology and Biotechnology
Volume36
Issue number6
Pages (from-to)863-871
Number of pages9
ISSN1367-5435
DOIs
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Fields of Science

  • Lactococcus lactis
  • Nisin
  • Regulation genes
  • Immunity genes
  • Continuous fermentation
  • SUBSP LACTIS
  • PLASMID STABILITY
  • BACTERIOCIN PRODUCTION
  • BATCH FERMENTATIONS
  • CONTINUOUS CULTURES
  • DILUTION RATE
  • BIOSYNTHESIS
  • GROWTH
  • CELLS
  • IMMOBILIZATION
  • 118 Biological sciences

Cite this

Simsek, O. ; Akkoc, N. ; Con, A. H. ; Ozcelik, F. ; Saris, P. E. J. ; Akcelik, Mustafa. / Continuous nisin production with bioengineered Lactococcus lactis strains. In: Journal of Industrial Microbiology and Biotechnology. 2009 ; Vol. 36, No. 6. pp. 863-871.
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abstract = "Nisin production in continuous cultures of bioengineered Lactococcus lactis strains that incorporate additional immunity and regulation genes was studied. Highest nisin activities were observed at 0.2 h(-1) dilution rate and 12.5 g l(-1) fructose concentration for all strains. Recombinant strains were able to produce greater amounts of nisin at dilution rates below 0.3 h(-1) compared to the control strain. However, this significant difference disappeared at dilution rates of 0.4 and 0.5 h(-1). For the strains LL27, LAC338, LAC339, and LAC340, optimum conditions for nisin production were determined to be at 0.29, 0.26, 0.27, and 0.27 h(-1) dilution rates and 11.95, 12.01, 11.63, and 12.50 g l(-1) fructose concentrations, respectively. The highest nisin productivity, 496 IU ml(-1) h(-1), was achieved with LAC339. The results of this study suggest that low dilution rates stabilize the high specific nisin productivity of the bioengineered strains in continuous fermentation. Moreover, response surface methodology analysis showed that regulation genes yielded high nisin productivity at wide ranges of dilution rates and fructose concentrations.",
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author = "O. Simsek and N. Akkoc and Con, {A. H.} and F. Ozcelik and Saris, {P. E. J.} and Mustafa Akcelik",
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Continuous nisin production with bioengineered Lactococcus lactis strains. / Simsek, O.; Akkoc, N.; Con, A. H.; Ozcelik, F.; Saris, P. E. J.; Akcelik, Mustafa.

In: Journal of Industrial Microbiology and Biotechnology, Vol. 36, No. 6, 2009, p. 863-871.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Continuous nisin production with bioengineered Lactococcus lactis strains

AU - Simsek, O.

AU - Akkoc, N.

AU - Con, A. H.

AU - Ozcelik, F.

AU - Saris, P. E. J.

AU - Akcelik, Mustafa

PY - 2009

Y1 - 2009

N2 - Nisin production in continuous cultures of bioengineered Lactococcus lactis strains that incorporate additional immunity and regulation genes was studied. Highest nisin activities were observed at 0.2 h(-1) dilution rate and 12.5 g l(-1) fructose concentration for all strains. Recombinant strains were able to produce greater amounts of nisin at dilution rates below 0.3 h(-1) compared to the control strain. However, this significant difference disappeared at dilution rates of 0.4 and 0.5 h(-1). For the strains LL27, LAC338, LAC339, and LAC340, optimum conditions for nisin production were determined to be at 0.29, 0.26, 0.27, and 0.27 h(-1) dilution rates and 11.95, 12.01, 11.63, and 12.50 g l(-1) fructose concentrations, respectively. The highest nisin productivity, 496 IU ml(-1) h(-1), was achieved with LAC339. The results of this study suggest that low dilution rates stabilize the high specific nisin productivity of the bioengineered strains in continuous fermentation. Moreover, response surface methodology analysis showed that regulation genes yielded high nisin productivity at wide ranges of dilution rates and fructose concentrations.

AB - Nisin production in continuous cultures of bioengineered Lactococcus lactis strains that incorporate additional immunity and regulation genes was studied. Highest nisin activities were observed at 0.2 h(-1) dilution rate and 12.5 g l(-1) fructose concentration for all strains. Recombinant strains were able to produce greater amounts of nisin at dilution rates below 0.3 h(-1) compared to the control strain. However, this significant difference disappeared at dilution rates of 0.4 and 0.5 h(-1). For the strains LL27, LAC338, LAC339, and LAC340, optimum conditions for nisin production were determined to be at 0.29, 0.26, 0.27, and 0.27 h(-1) dilution rates and 11.95, 12.01, 11.63, and 12.50 g l(-1) fructose concentrations, respectively. The highest nisin productivity, 496 IU ml(-1) h(-1), was achieved with LAC339. The results of this study suggest that low dilution rates stabilize the high specific nisin productivity of the bioengineered strains in continuous fermentation. Moreover, response surface methodology analysis showed that regulation genes yielded high nisin productivity at wide ranges of dilution rates and fructose concentrations.

KW - Lactococcus lactis

KW - Nisin

KW - Regulation genes

KW - Immunity genes

KW - Continuous fermentation

KW - SUBSP LACTIS

KW - PLASMID STABILITY

KW - BACTERIOCIN PRODUCTION

KW - BATCH FERMENTATIONS

KW - CONTINUOUS CULTURES

KW - DILUTION RATE

KW - BIOSYNTHESIS

KW - GROWTH

KW - CELLS

KW - IMMOBILIZATION

KW - 118 Biological sciences

U2 - 10.1007/s10295-009-0563-6

DO - 10.1007/s10295-009-0563-6

M3 - Article

VL - 36

SP - 863

EP - 871

JO - Journal of Industrial Microbiology and Biotechnology

JF - Journal of Industrial Microbiology and Biotechnology

SN - 1367-5435

IS - 6

ER -