Conversion of biowaste leachate to valuable biomass and lipids in mixed cultures of Euglena gracilis and chlorophytes

Marika Tossavainen, Neha Katyal, Kostia Silja, Kalle Valkonen, Anil K. Sharma, Suvigya Sharma, Anne Ojala, Martin Romantschuk

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Microalgae are a sustainable alternative for production of valuable omega -3 fatty acids (FAs), but high production costs limit commercialization. Utilization of waste as a nutrient source increases the economics of the cultivation process. Additionally, using mixed algal cultures instead of monocultures makes the cultivation process more flexible and can increase biomass and lipid production. Here, the growth and lipid production of microalgae Euglena gracilis, Selenastrum sp. and, Chlorella sorokiniana were studied in mono- and mixed cultures in small and pilot scale experiments in biowaste leachate. In pilot scale, also nutrient reduction and the number of bacteria were analyzed. Biomass production in the most productive mixed cultures was similar, but not higher than in most productive monocultures. The lipid production was highest in the small-scale monoculture of Selenastrum (10.4% DW) and in the pilot scale culture of Selenastrum with E. gracilis (11.1% DW). The content of alpha-linolenic acid (ALA) increased and eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) remained stable during the cultivation period in all pilot scale cultures. However, increases in biomass and lipid production toward the end of the cultivation resulted in higher EPA and DHA yields in the well growing monoculture of E. gracilis and in the mixed culture of E. gracilis with Selenastrum. Co-cultivation of E. gracilis and Selenastrum also had a positive influence on nutrient uptake and resistance against bacteria. This type of mixed culture may be a good option for commercialization. However, as shown here, minor changes in cultivation conditions can rapidly result in dominance of a subdominant strain, and thus the stability of strain performance and production of desired FAs needs further investigation.
Original languageEnglish
JournalAlgal Research
Volume35
Pages (from-to)76-84
Number of pages9
ISSN2211-9264
DOIs
Publication statusPublished - Nov 2018
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1172 Environmental sciences
  • 1183 Plant biology, microbiology, virology
  • LC-PUFA
  • EPA
  • DHA
  • Nutrient
  • Bacteria
  • Overyielding
  • N-3 FATTY-ACIDS
  • WASTE-WATER
  • MICROALGAL CULTIVATION
  • CHLORELLA-SOROKINIANA
  • EFFLUENT
  • GROWTH
  • CARBON
  • TRIACYLGLYCEROLS
  • BIOFUEL

Cite this

@article{53c5a8098a534225856c27f82358d7b2,
title = "Conversion of biowaste leachate to valuable biomass and lipids in mixed cultures of Euglena gracilis and chlorophytes",
abstract = "Microalgae are a sustainable alternative for production of valuable omega -3 fatty acids (FAs), but high production costs limit commercialization. Utilization of waste as a nutrient source increases the economics of the cultivation process. Additionally, using mixed algal cultures instead of monocultures makes the cultivation process more flexible and can increase biomass and lipid production. Here, the growth and lipid production of microalgae Euglena gracilis, Selenastrum sp. and, Chlorella sorokiniana were studied in mono- and mixed cultures in small and pilot scale experiments in biowaste leachate. In pilot scale, also nutrient reduction and the number of bacteria were analyzed. Biomass production in the most productive mixed cultures was similar, but not higher than in most productive monocultures. The lipid production was highest in the small-scale monoculture of Selenastrum (10.4{\%} DW) and in the pilot scale culture of Selenastrum with E. gracilis (11.1{\%} DW). The content of alpha-linolenic acid (ALA) increased and eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) remained stable during the cultivation period in all pilot scale cultures. However, increases in biomass and lipid production toward the end of the cultivation resulted in higher EPA and DHA yields in the well growing monoculture of E. gracilis and in the mixed culture of E. gracilis with Selenastrum. Co-cultivation of E. gracilis and Selenastrum also had a positive influence on nutrient uptake and resistance against bacteria. This type of mixed culture may be a good option for commercialization. However, as shown here, minor changes in cultivation conditions can rapidly result in dominance of a subdominant strain, and thus the stability of strain performance and production of desired FAs needs further investigation.",
keywords = "1172 Environmental sciences, 1183 Plant biology, microbiology, virology, LC-PUFA, EPA, DHA, Nutrient, Bacteria, Overyielding, N-3 FATTY-ACIDS, WASTE-WATER, MICROALGAL CULTIVATION, CHLORELLA-SOROKINIANA, EFFLUENT, GROWTH, CARBON, TRIACYLGLYCEROLS, BIOFUEL",
author = "Marika Tossavainen and Neha Katyal and Kostia Silja and Kalle Valkonen and Sharma, {Anil K.} and Suvigya Sharma and Anne Ojala and Martin Romantschuk",
year = "2018",
month = "11",
doi = "10.1016/j.algal.2018.08.007",
language = "English",
volume = "35",
pages = "76--84",
journal = "Algal Research",
issn = "2211-9264",
publisher = "Elsevier Scientific Publ. Co",

}

Conversion of biowaste leachate to valuable biomass and lipids in mixed cultures of Euglena gracilis and chlorophytes. / Tossavainen, Marika; Katyal, Neha; Silja, Kostia; Valkonen, Kalle; Sharma, Anil K.; Sharma, Suvigya; Ojala, Anne; Romantschuk, Martin.

In: Algal Research, Vol. 35, 11.2018, p. 76-84.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Conversion of biowaste leachate to valuable biomass and lipids in mixed cultures of Euglena gracilis and chlorophytes

AU - Tossavainen, Marika

AU - Katyal, Neha

AU - Silja, Kostia

AU - Valkonen, Kalle

AU - Sharma, Anil K.

AU - Sharma, Suvigya

AU - Ojala, Anne

AU - Romantschuk, Martin

PY - 2018/11

Y1 - 2018/11

N2 - Microalgae are a sustainable alternative for production of valuable omega -3 fatty acids (FAs), but high production costs limit commercialization. Utilization of waste as a nutrient source increases the economics of the cultivation process. Additionally, using mixed algal cultures instead of monocultures makes the cultivation process more flexible and can increase biomass and lipid production. Here, the growth and lipid production of microalgae Euglena gracilis, Selenastrum sp. and, Chlorella sorokiniana were studied in mono- and mixed cultures in small and pilot scale experiments in biowaste leachate. In pilot scale, also nutrient reduction and the number of bacteria were analyzed. Biomass production in the most productive mixed cultures was similar, but not higher than in most productive monocultures. The lipid production was highest in the small-scale monoculture of Selenastrum (10.4% DW) and in the pilot scale culture of Selenastrum with E. gracilis (11.1% DW). The content of alpha-linolenic acid (ALA) increased and eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) remained stable during the cultivation period in all pilot scale cultures. However, increases in biomass and lipid production toward the end of the cultivation resulted in higher EPA and DHA yields in the well growing monoculture of E. gracilis and in the mixed culture of E. gracilis with Selenastrum. Co-cultivation of E. gracilis and Selenastrum also had a positive influence on nutrient uptake and resistance against bacteria. This type of mixed culture may be a good option for commercialization. However, as shown here, minor changes in cultivation conditions can rapidly result in dominance of a subdominant strain, and thus the stability of strain performance and production of desired FAs needs further investigation.

AB - Microalgae are a sustainable alternative for production of valuable omega -3 fatty acids (FAs), but high production costs limit commercialization. Utilization of waste as a nutrient source increases the economics of the cultivation process. Additionally, using mixed algal cultures instead of monocultures makes the cultivation process more flexible and can increase biomass and lipid production. Here, the growth and lipid production of microalgae Euglena gracilis, Selenastrum sp. and, Chlorella sorokiniana were studied in mono- and mixed cultures in small and pilot scale experiments in biowaste leachate. In pilot scale, also nutrient reduction and the number of bacteria were analyzed. Biomass production in the most productive mixed cultures was similar, but not higher than in most productive monocultures. The lipid production was highest in the small-scale monoculture of Selenastrum (10.4% DW) and in the pilot scale culture of Selenastrum with E. gracilis (11.1% DW). The content of alpha-linolenic acid (ALA) increased and eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) remained stable during the cultivation period in all pilot scale cultures. However, increases in biomass and lipid production toward the end of the cultivation resulted in higher EPA and DHA yields in the well growing monoculture of E. gracilis and in the mixed culture of E. gracilis with Selenastrum. Co-cultivation of E. gracilis and Selenastrum also had a positive influence on nutrient uptake and resistance against bacteria. This type of mixed culture may be a good option for commercialization. However, as shown here, minor changes in cultivation conditions can rapidly result in dominance of a subdominant strain, and thus the stability of strain performance and production of desired FAs needs further investigation.

KW - 1172 Environmental sciences

KW - 1183 Plant biology, microbiology, virology

KW - LC-PUFA

KW - EPA

KW - DHA

KW - Nutrient

KW - Bacteria

KW - Overyielding

KW - N-3 FATTY-ACIDS

KW - WASTE-WATER

KW - MICROALGAL CULTIVATION

KW - CHLORELLA-SOROKINIANA

KW - EFFLUENT

KW - GROWTH

KW - CARBON

KW - TRIACYLGLYCEROLS

KW - BIOFUEL

U2 - 10.1016/j.algal.2018.08.007

DO - 10.1016/j.algal.2018.08.007

M3 - Article

VL - 35

SP - 76

EP - 84

JO - Algal Research

JF - Algal Research

SN - 2211-9264

ER -