Abstract

Effect of three wood-decaying fungi on decomposition of spruce wood was studied in solid-state cultivation conditions for a period of three months. Two white rot species (Trichaptum abietinum and Phlebia radiata) were challenged by a brown rot species (Fomitopsis pinicola) in varying combinations. Wood decomposition patterns as determined by mass loss, carbon to nitrogen ratio, accumulation of dissolved sugars, and release of volatile organic compounds (VOCs) were observed to depend on both fungal combinations and growth time. Similar dependence of fungal species combination, either white or brown rot dominated, was observed for secreted enzyme activities on spruce wood. Fenton chemistry suggesting reduction of Fe3+ to Fe2+ was detected in the presence of F. pinicola, even in co-cultures, together with substantial degradation of wood carbohydrates and accumulation of oxalic acid. Significant correlation was perceived with two enzyme activity patterns (oxidoreductases produced by white rot fungi; hydrolytic enzymes produced by the brown rot fungus) and wood degradation efficiency. Moreover, emission of four signature VOCs clearly grouped the fungal combinations. Our results indicate that fungal decay type, either brown or white rot, determines the loss of wood mass and decomposition of polysaccharides as well as the pattern of VOCs released upon fungal growth on spruce wood.
Original languageEnglish
Article number135
JournalFEMS Microbiology Ecology
Volume95
Issue number9
Number of pages13
ISSN0168-6496
DOIs
Publication statusPublished - Sep 2019
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 1181 Ecology, evolutionary biology
  • Fungal ecology
  • 1172 Environmental sciences
  • Carbon cycle
  • Volatile organic compounds (VOC)
  • 4112 Forestry
  • Forest ecology
  • Wood biomass
  • Wood decay fungi
  • 1183 Plant biology, microbiology, virology
  • Fungal biology
  • Mycology
  • Environmental microbiology
  • wood-decaying fungi
  • wood biodegradation
  • white rot
  • brown rot
  • VOCs
  • CAZymes
  • oxalic acid
  • iron reduction
  • Agaricomycetes
  • Fomitopsis pinicola
  • Phlebia radiata
  • Trichaptum abietinum
  • BROWN-ROT FUNGI
  • LIGNIN MODEL COMPOUNDS
  • CONIOPHORA-PUTEANA
  • SERPULA-LACRYMANS
  • PHLEBIA-RADIATA
  • SINGLE-STEP
  • DEGRADATION
  • EMISSIONS
  • CELLULOSE
  • SOIL

Cite this

@article{e104520e2a7e4d59bb560dfa3ea8f28d,
title = "Decomposition of spruce wood and release of volatile organic compounds depend on decay type, fungal interactions and enzyme production patterns",
abstract = "Effect of three wood-decaying fungi on decomposition of spruce wood was studied in solid-state cultivation conditions for a period of three months. Two white rot species (Trichaptum abietinum and Phlebia radiata) were challenged by a brown rot species (Fomitopsis pinicola) in varying combinations. Wood decomposition patterns as determined by mass loss, carbon to nitrogen ratio, accumulation of dissolved sugars, and release of volatile organic compounds (VOCs) were observed to depend on both fungal combinations and growth time. Similar dependence of fungal species combination, either white or brown rot dominated, was observed for secreted enzyme activities on spruce wood. Fenton chemistry suggesting reduction of Fe3+ to Fe2+ was detected in the presence of F. pinicola, even in co-cultures, together with substantial degradation of wood carbohydrates and accumulation of oxalic acid. Significant correlation was perceived with two enzyme activity patterns (oxidoreductases produced by white rot fungi; hydrolytic enzymes produced by the brown rot fungus) and wood degradation efficiency. Moreover, emission of four signature VOCs clearly grouped the fungal combinations. Our results indicate that fungal decay type, either brown or white rot, determines the loss of wood mass and decomposition of polysaccharides as well as the pattern of VOCs released upon fungal growth on spruce wood.",
keywords = "1181 Ecology, evolutionary biology, Fungal ecology, 1172 Environmental sciences, Carbon cycle, Volatile organic compounds (VOC), 4112 Forestry, Forest ecology, Wood biomass, Wood decay fungi, 1183 Plant biology, microbiology, virology, Fungal biology, Mycology, Environmental microbiology, wood-decaying fungi, wood biodegradation, white rot, brown rot, VOCs, CAZymes, oxalic acid, iron reduction, Agaricomycetes, Fomitopsis pinicola, Phlebia radiata, Trichaptum abietinum, BROWN-ROT FUNGI, LIGNIN MODEL COMPOUNDS, CONIOPHORA-PUTEANA, SERPULA-LACRYMANS, PHLEBIA-RADIATA, SINGLE-STEP, DEGRADATION, EMISSIONS, CELLULOSE, SOIL",
author = "Tuulia Mali and Mari M{\"a}ki and Heidi Hell{\'e}n and Jussi Heinonsalo and Jaana B{\"a}ck and Taina Lundell",
year = "2019",
month = "9",
doi = "10.1093/femsec/fiz135",
language = "English",
volume = "95",
journal = "FEMS Microbiology Ecology",
issn = "0168-6496",
publisher = "Oxford University Press",
number = "9",

}

TY - JOUR

T1 - Decomposition of spruce wood and release of volatile organic compounds depend on decay type, fungal interactions and enzyme production patterns

AU - Mali, Tuulia

AU - Mäki, Mari

AU - Hellén, Heidi

AU - Heinonsalo, Jussi

AU - Bäck, Jaana

AU - Lundell, Taina

PY - 2019/9

Y1 - 2019/9

N2 - Effect of three wood-decaying fungi on decomposition of spruce wood was studied in solid-state cultivation conditions for a period of three months. Two white rot species (Trichaptum abietinum and Phlebia radiata) were challenged by a brown rot species (Fomitopsis pinicola) in varying combinations. Wood decomposition patterns as determined by mass loss, carbon to nitrogen ratio, accumulation of dissolved sugars, and release of volatile organic compounds (VOCs) were observed to depend on both fungal combinations and growth time. Similar dependence of fungal species combination, either white or brown rot dominated, was observed for secreted enzyme activities on spruce wood. Fenton chemistry suggesting reduction of Fe3+ to Fe2+ was detected in the presence of F. pinicola, even in co-cultures, together with substantial degradation of wood carbohydrates and accumulation of oxalic acid. Significant correlation was perceived with two enzyme activity patterns (oxidoreductases produced by white rot fungi; hydrolytic enzymes produced by the brown rot fungus) and wood degradation efficiency. Moreover, emission of four signature VOCs clearly grouped the fungal combinations. Our results indicate that fungal decay type, either brown or white rot, determines the loss of wood mass and decomposition of polysaccharides as well as the pattern of VOCs released upon fungal growth on spruce wood.

AB - Effect of three wood-decaying fungi on decomposition of spruce wood was studied in solid-state cultivation conditions for a period of three months. Two white rot species (Trichaptum abietinum and Phlebia radiata) were challenged by a brown rot species (Fomitopsis pinicola) in varying combinations. Wood decomposition patterns as determined by mass loss, carbon to nitrogen ratio, accumulation of dissolved sugars, and release of volatile organic compounds (VOCs) were observed to depend on both fungal combinations and growth time. Similar dependence of fungal species combination, either white or brown rot dominated, was observed for secreted enzyme activities on spruce wood. Fenton chemistry suggesting reduction of Fe3+ to Fe2+ was detected in the presence of F. pinicola, even in co-cultures, together with substantial degradation of wood carbohydrates and accumulation of oxalic acid. Significant correlation was perceived with two enzyme activity patterns (oxidoreductases produced by white rot fungi; hydrolytic enzymes produced by the brown rot fungus) and wood degradation efficiency. Moreover, emission of four signature VOCs clearly grouped the fungal combinations. Our results indicate that fungal decay type, either brown or white rot, determines the loss of wood mass and decomposition of polysaccharides as well as the pattern of VOCs released upon fungal growth on spruce wood.

KW - 1181 Ecology, evolutionary biology

KW - Fungal ecology

KW - 1172 Environmental sciences

KW - Carbon cycle

KW - Volatile organic compounds (VOC)

KW - 4112 Forestry

KW - Forest ecology

KW - Wood biomass

KW - Wood decay fungi

KW - 1183 Plant biology, microbiology, virology

KW - Fungal biology

KW - Mycology

KW - Environmental microbiology

KW - wood-decaying fungi

KW - wood biodegradation

KW - white rot

KW - brown rot

KW - VOCs

KW - CAZymes

KW - oxalic acid

KW - iron reduction

KW - Agaricomycetes

KW - Fomitopsis pinicola

KW - Phlebia radiata

KW - Trichaptum abietinum

KW - BROWN-ROT FUNGI

KW - LIGNIN MODEL COMPOUNDS

KW - CONIOPHORA-PUTEANA

KW - SERPULA-LACRYMANS

KW - PHLEBIA-RADIATA

KW - SINGLE-STEP

KW - DEGRADATION

KW - EMISSIONS

KW - CELLULOSE

KW - SOIL

U2 - 10.1093/femsec/fiz135

DO - 10.1093/femsec/fiz135

M3 - Article

VL - 95

JO - FEMS Microbiology Ecology

JF - FEMS Microbiology Ecology

SN - 0168-6496

IS - 9

M1 - 135

ER -