Desorption electrospray ionization mass spectrometry for the analysis of pharmaceuticals and metabolites

Tiina Kauppila, Justin Wiseman, Raimo A Ketola, Tapio Kotiaho, R. Graham Cooks, Risto Kostiainen

    Research output: Contribution to journalArticleScientificpeer-review

    Abstract

    "The performance of desorption electrospray ionization (DESI) in the analysis of a group of pharmaceuticals and their glucuronic acid conjugates is reported. The suitability of different sprayer solvents and different surfaces was examined. In the positive ion mode, water/methanol/trifluoroacetic acid performed best, whereas, in the negative ion mode, water/methanol/ammonium hydroxide was found to be the most suitable spray solvent. Of the surfaces investigated, polymethylmethacrylate (PMMA) was found to give the best performance in terms of sensitivity. Spray solution flow rate and the distance of the sprayer tip from the surface were also found to have significant effects on the signal intensity. Analytes with basic groups efficiently formed the corresponding protonated molecules in the positive ion mode, whereas acidic analytes, such as the glucuronic acid conjugates, formed intense signals due to the deprotonated molecules in the negative ion mode. Ionization of neutral compounds was less efficient and in many cases it was achieved through adduct formation with simple anions or cations. Copyright (c) 2005 John Wiley & Sons, Ltd."
    Original languageEnglish
    JournalRapid Communications in Mass Spectrometry
    Volume20
    Issue number3
    Pages (from-to)387-392
    ISSN0951-4198
    DOIs
    Publication statusPublished - 2006
    MoE publication typeA1 Journal article-refereed

    Cite this

    @article{f76e3df2413c40a29696815f0679a606,
    title = "Desorption electrospray ionization mass spectrometry for the analysis of pharmaceuticals and metabolites",
    abstract = "{"}The performance of desorption electrospray ionization (DESI) in the analysis of a group of pharmaceuticals and their glucuronic acid conjugates is reported. The suitability of different sprayer solvents and different surfaces was examined. In the positive ion mode, water/methanol/trifluoroacetic acid performed best, whereas, in the negative ion mode, water/methanol/ammonium hydroxide was found to be the most suitable spray solvent. Of the surfaces investigated, polymethylmethacrylate (PMMA) was found to give the best performance in terms of sensitivity. Spray solution flow rate and the distance of the sprayer tip from the surface were also found to have significant effects on the signal intensity. Analytes with basic groups efficiently formed the corresponding protonated molecules in the positive ion mode, whereas acidic analytes, such as the glucuronic acid conjugates, formed intense signals due to the deprotonated molecules in the negative ion mode. Ionization of neutral compounds was less efficient and in many cases it was achieved through adduct formation with simple anions or cations. Copyright (c) 2005 John Wiley & Sons, Ltd.{"}",
    author = "Tiina Kauppila and Justin Wiseman and Ketola, {Raimo A} and Tapio Kotiaho and Cooks, {R. Graham} and Risto Kostiainen",
    year = "2006",
    doi = "10.1002/rcm.2304",
    language = "English",
    volume = "20",
    pages = "387--392",
    journal = "Rapid Communications in Mass Spectrometry",
    issn = "0951-4198",
    publisher = "John Wiley & Sons Ltd.",
    number = "3",

    }

    Desorption electrospray ionization mass spectrometry for the analysis of pharmaceuticals and metabolites. / Kauppila, Tiina; Wiseman, Justin; Ketola, Raimo A; Kotiaho, Tapio; Cooks, R. Graham; Kostiainen, Risto.

    In: Rapid Communications in Mass Spectrometry, Vol. 20, No. 3, 2006, p. 387-392.

    Research output: Contribution to journalArticleScientificpeer-review

    TY - JOUR

    T1 - Desorption electrospray ionization mass spectrometry for the analysis of pharmaceuticals and metabolites

    AU - Kauppila, Tiina

    AU - Wiseman, Justin

    AU - Ketola, Raimo A

    AU - Kotiaho, Tapio

    AU - Cooks, R. Graham

    AU - Kostiainen, Risto

    PY - 2006

    Y1 - 2006

    N2 - "The performance of desorption electrospray ionization (DESI) in the analysis of a group of pharmaceuticals and their glucuronic acid conjugates is reported. The suitability of different sprayer solvents and different surfaces was examined. In the positive ion mode, water/methanol/trifluoroacetic acid performed best, whereas, in the negative ion mode, water/methanol/ammonium hydroxide was found to be the most suitable spray solvent. Of the surfaces investigated, polymethylmethacrylate (PMMA) was found to give the best performance in terms of sensitivity. Spray solution flow rate and the distance of the sprayer tip from the surface were also found to have significant effects on the signal intensity. Analytes with basic groups efficiently formed the corresponding protonated molecules in the positive ion mode, whereas acidic analytes, such as the glucuronic acid conjugates, formed intense signals due to the deprotonated molecules in the negative ion mode. Ionization of neutral compounds was less efficient and in many cases it was achieved through adduct formation with simple anions or cations. Copyright (c) 2005 John Wiley & Sons, Ltd."

    AB - "The performance of desorption electrospray ionization (DESI) in the analysis of a group of pharmaceuticals and their glucuronic acid conjugates is reported. The suitability of different sprayer solvents and different surfaces was examined. In the positive ion mode, water/methanol/trifluoroacetic acid performed best, whereas, in the negative ion mode, water/methanol/ammonium hydroxide was found to be the most suitable spray solvent. Of the surfaces investigated, polymethylmethacrylate (PMMA) was found to give the best performance in terms of sensitivity. Spray solution flow rate and the distance of the sprayer tip from the surface were also found to have significant effects on the signal intensity. Analytes with basic groups efficiently formed the corresponding protonated molecules in the positive ion mode, whereas acidic analytes, such as the glucuronic acid conjugates, formed intense signals due to the deprotonated molecules in the negative ion mode. Ionization of neutral compounds was less efficient and in many cases it was achieved through adduct formation with simple anions or cations. Copyright (c) 2005 John Wiley & Sons, Ltd."

    U2 - 10.1002/rcm.2304

    DO - 10.1002/rcm.2304

    M3 - Article

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    SP - 387

    EP - 392

    JO - Rapid Communications in Mass Spectrometry

    JF - Rapid Communications in Mass Spectrometry

    SN - 0951-4198

    IS - 3

    ER -