Detection and quantitation of colored deposit-forming Meiothermus spp. in paper industry processes and end products

Jaakko Ekman, Mirva Kosonen, Sanna Jokela, Marko Kolari, Päivi Korhonen, Mirja Salkinoja-Salonen

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"Colored biofilms cause problems in paper industry. In this work we used real-time PCR to detect and to quantitate members of the genus Meiothermus from the process samples and end products from 24 machines manufacturing pulp, paper and board in four countries. The results obtained from 200 samples showed the importance of members of the genus Meiothermus as ubiquitous biofoulers in paper machines. This genus was the dominant biofouler in some mills. From <= 10(4) to 10(11) copies of Meiothermus 16S rRNA genes were found per gram of process deposit (wet weight). Meiothermus spp. were found in paper and board products with colored defects and connection between deposit-forming microbes and end-product spots was shown. 16S rRNA gene sequences of 29 biofilm producing bacterial isolates from different mills were determined. Based on sequence data, 25 of the isolates were assigned to the genus Meiothermus, with Meiothermus silvanus and M. ruber as the most frequent species."
Original languageEnglish
JournalJournal of Industrial Microbiology and Biotechnology
Issue number3
Pages (from-to)203-211
Number of pages9
Publication statusPublished - 2007
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 119 Other natural sciences

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