Development of IRAP- and REMAP-derived SCAR markers for marker-assisted selection of the strip rust resistance gene Yr15 derived from wild emmer wheat

Babak Abdollahi Mandoulakani, Elitsur Yaniv, Ruslan Kalendar, Dina Raats, Harbans S. Bariana, Mohammad Reza Bihamta, Alan Schulman

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Stripe rust (Pucinia striformis f.sp. tritici) is one of the most important fungal diseases of wheat, found on all continents and in over 60 countries. Wild emmer wheat, Triticum dicoccoides, which is the tetraploid progenitor of durum wheat, is a valuable source of novel stripe rust resistance genes for wheat breeding. T. dicoccoides G25 accession carries Yr15, a gene on chromosome arm 1BS. Yr15 confers resistance to all known stripe rust isolates; it is also effective in introgressed durum and bread wheat. Retrotransposons generate polymorphic insertions, which can be scored as Mendelian markers with techniques including REMAP and IRAP. Six REMAP and IRAP-derived SCAR markers were developed using 1256 F2 plants derived from crosses of the susceptible T. durum accession D447 with its resistant BC3F9 and BC3F10 (B9 and B10) near isogenic lines, which carried Yr15 introgressed from G25. The nearest markers segregated 0.1 cM proximally and 1.1 cM distally to Yr15. These markers were also mapped and validated at the same position in another independent 500 F2 plants derived from crosses of B9 and B10 with the susceptible cultivar Langdon. SCAR270 and SCAR790, surrounding Yr15 at an interval of 1.2 cM, were found to be reliable and robust co-dominant markers in a wide range of wheat lines and cultivars with and without Yr15. These markers are useful tags in marker-assisted wheat breeding programs aiming to incorporate Yr15 into elite wheat lines and cultivars for durable and broad-spectrum resistance against stripe rust.
Original languageEnglish
JournalTheoretical and Applied Genetics
Volume128
Issue number2
Pages (from-to)211-219
Number of pages9
ISSN0040-5752
DOIs
Publication statusPublished - 1 Feb 2015
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 4111 Agronomy
  • 1183 Plant biology, microbiology, virology

Cite this

Abdollahi Mandoulakani, Babak ; Yaniv, Elitsur ; Kalendar, Ruslan ; Raats, Dina ; Bariana, Harbans S. ; Reza Bihamta, Mohammad ; Schulman, Alan. / Development of IRAP- and REMAP-derived SCAR markers for marker-assisted selection of the strip rust resistance gene Yr15 derived from wild emmer wheat. In: Theoretical and Applied Genetics. 2015 ; Vol. 128, No. 2. pp. 211-219.
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title = "Development of IRAP- and REMAP-derived SCAR markers for marker-assisted selection of the strip rust resistance gene Yr15 derived from wild emmer wheat",
abstract = "Stripe rust (Pucinia striformis f.sp. tritici) is one of the most important fungal diseases of wheat, found on all continents and in over 60 countries. Wild emmer wheat, Triticum dicoccoides, which is the tetraploid progenitor of durum wheat, is a valuable source of novel stripe rust resistance genes for wheat breeding. T. dicoccoides G25 accession carries Yr15, a gene on chromosome arm 1BS. Yr15 confers resistance to all known stripe rust isolates; it is also effective in introgressed durum and bread wheat. Retrotransposons generate polymorphic insertions, which can be scored as Mendelian markers with techniques including REMAP and IRAP. Six REMAP and IRAP-derived SCAR markers were developed using 1256 F2 plants derived from crosses of the susceptible T. durum accession D447 with its resistant BC3F9 and BC3F10 (B9 and B10) near isogenic lines, which carried Yr15 introgressed from G25. The nearest markers segregated 0.1 cM proximally and 1.1 cM distally to Yr15. These markers were also mapped and validated at the same position in another independent 500 F2 plants derived from crosses of B9 and B10 with the susceptible cultivar Langdon. SCAR270 and SCAR790, surrounding Yr15 at an interval of 1.2 cM, were found to be reliable and robust co-dominant markers in a wide range of wheat lines and cultivars with and without Yr15. These markers are useful tags in marker-assisted wheat breeding programs aiming to incorporate Yr15 into elite wheat lines and cultivars for durable and broad-spectrum resistance against stripe rust.",
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author = "{Abdollahi Mandoulakani}, Babak and Elitsur Yaniv and Ruslan Kalendar and Dina Raats and Bariana, {Harbans S.} and {Reza Bihamta}, Mohammad and Alan Schulman",
year = "2015",
month = "2",
day = "1",
doi = "10.1007/s00122-014-2422-8",
language = "English",
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Development of IRAP- and REMAP-derived SCAR markers for marker-assisted selection of the strip rust resistance gene Yr15 derived from wild emmer wheat. / Abdollahi Mandoulakani, Babak; Yaniv, Elitsur; Kalendar, Ruslan; Raats, Dina; Bariana, Harbans S.; Reza Bihamta, Mohammad; Schulman, Alan.

In: Theoretical and Applied Genetics, Vol. 128, No. 2, 01.02.2015, p. 211-219.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Development of IRAP- and REMAP-derived SCAR markers for marker-assisted selection of the strip rust resistance gene Yr15 derived from wild emmer wheat

AU - Abdollahi Mandoulakani, Babak

AU - Yaniv, Elitsur

AU - Kalendar, Ruslan

AU - Raats, Dina

AU - Bariana, Harbans S.

AU - Reza Bihamta, Mohammad

AU - Schulman, Alan

PY - 2015/2/1

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N2 - Stripe rust (Pucinia striformis f.sp. tritici) is one of the most important fungal diseases of wheat, found on all continents and in over 60 countries. Wild emmer wheat, Triticum dicoccoides, which is the tetraploid progenitor of durum wheat, is a valuable source of novel stripe rust resistance genes for wheat breeding. T. dicoccoides G25 accession carries Yr15, a gene on chromosome arm 1BS. Yr15 confers resistance to all known stripe rust isolates; it is also effective in introgressed durum and bread wheat. Retrotransposons generate polymorphic insertions, which can be scored as Mendelian markers with techniques including REMAP and IRAP. Six REMAP and IRAP-derived SCAR markers were developed using 1256 F2 plants derived from crosses of the susceptible T. durum accession D447 with its resistant BC3F9 and BC3F10 (B9 and B10) near isogenic lines, which carried Yr15 introgressed from G25. The nearest markers segregated 0.1 cM proximally and 1.1 cM distally to Yr15. These markers were also mapped and validated at the same position in another independent 500 F2 plants derived from crosses of B9 and B10 with the susceptible cultivar Langdon. SCAR270 and SCAR790, surrounding Yr15 at an interval of 1.2 cM, were found to be reliable and robust co-dominant markers in a wide range of wheat lines and cultivars with and without Yr15. These markers are useful tags in marker-assisted wheat breeding programs aiming to incorporate Yr15 into elite wheat lines and cultivars for durable and broad-spectrum resistance against stripe rust.

AB - Stripe rust (Pucinia striformis f.sp. tritici) is one of the most important fungal diseases of wheat, found on all continents and in over 60 countries. Wild emmer wheat, Triticum dicoccoides, which is the tetraploid progenitor of durum wheat, is a valuable source of novel stripe rust resistance genes for wheat breeding. T. dicoccoides G25 accession carries Yr15, a gene on chromosome arm 1BS. Yr15 confers resistance to all known stripe rust isolates; it is also effective in introgressed durum and bread wheat. Retrotransposons generate polymorphic insertions, which can be scored as Mendelian markers with techniques including REMAP and IRAP. Six REMAP and IRAP-derived SCAR markers were developed using 1256 F2 plants derived from crosses of the susceptible T. durum accession D447 with its resistant BC3F9 and BC3F10 (B9 and B10) near isogenic lines, which carried Yr15 introgressed from G25. The nearest markers segregated 0.1 cM proximally and 1.1 cM distally to Yr15. These markers were also mapped and validated at the same position in another independent 500 F2 plants derived from crosses of B9 and B10 with the susceptible cultivar Langdon. SCAR270 and SCAR790, surrounding Yr15 at an interval of 1.2 cM, were found to be reliable and robust co-dominant markers in a wide range of wheat lines and cultivars with and without Yr15. These markers are useful tags in marker-assisted wheat breeding programs aiming to incorporate Yr15 into elite wheat lines and cultivars for durable and broad-spectrum resistance against stripe rust.

KW - 4111 Agronomy

KW - 1183 Plant biology, microbiology, virology

U2 - 10.1007/s00122-014-2422-8

DO - 10.1007/s00122-014-2422-8

M3 - Article

VL - 128

SP - 211

EP - 219

JO - Theoretical and Applied Genetics

JF - Theoretical and Applied Genetics

SN - 0040-5752

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ER -