Dietary supplement of conjugated linoleic acid and polyunsaturated fatty acid suppressed the mobilization of body fat reserves from subcutaneous adipose tissue in dairy cows at early lactation through different pathways

Nanbing Qin, Ali-Reza Bayat, Piia Kairenius, Sirja Viitala, Mervi Mutikainen, Heidi Leskinen, Kari Tapani Elo, Tuomo Juhani Kokkonen, Johanna Vilkki

Research output: Conference materialsPosterResearchpeer-review

Abstract

Milk fat depression (MFD) is an effective way to reduce the metabolic stress of dairy cows during lactation. Dietary supplement of
fatty acids can induce MFD by inhibiting mammary lipid synthesis. To investigate the impacts of MFD on the metabolism in the
adipose tissue (AT), 30 cows were randomly assigned to a control diet, a conjugated linoleic acid supplemented diet (as CLA diet),
or a high-starch diet supplemented with polyunsaturated fatty acids (sunflower oil: fish oil = 2:1; as HSO diet). Measurements of
animal performance, milk yield, milk composition, energy balance, and blood metabolites were conducted at intervals during
lactation. The expression of candidate genes in AT was analyzed by quantitative PCR at wk 3 and 15 of lactation. Milk fat yield and
content were effectively depressed by the CLA and HSO diets at wk 3 and wk 15 of lactation. Surprisingly, milk yield was also
considerably decreased in the HSO group. The CLA and HSO diets improved energy balance at early lactation, supported by the
reduced body weight loss and decreased plasma non-esterified fatty acid and β-hydroxybutyrate concentrations in these two
groups. Lipolysis in AT was suppressed by the CLA and HSO diets at early lactation through the downregulation of hormonesensitive
lipase and fatty acid binding protein 4 and the upregulation of perilipin 2. Lipogenesis in AT was promoted only by the
HSO diet at wk 15 through the upregulation of 1-acylglycerol-3-phosphate O-acyltransferase 2, mitochondrial glycerol-3-phosphate
acyltransferase, perilipin 2, and peroxisome proliferator-activated receptor γ. In addition, the CLA diet led to increasing
transcription of various genes involved in insulin signaling, inflammatory responses, and ceramide metabolism, including protein
kinase B2, nuclear factor κ B1, toll-like receptor 4, caveolin 1, serine palmitoyltransferase long chain base subunit 1, and Nacylsphingosine
amidohydrolase 1. The changes in these insulin-related pathways suggest likely altered insulin sensitivity in AT of
the CLA group. In contrast, the HSO diet resulted in little or no change in these insulin-related pathways. In conclusion, the CLA and
HSO diets shifted energy partitioning towards AT instead of mammary gland at early lactation through the regulation of different
pathways.
Original languageEnglish
PagesP31
Number of pages1
Publication statusPublished - Mar 2018
MoE publication typeNot Eligible
EventFifth DairyCare Conference -
Duration: 19 Mar 201820 Mar 2018

Conference

ConferenceFifth DairyCare Conference
Period19/03/201820/03/2018

Fields of Science

  • 412 Animal science, dairy science

Cite this

Qin, N., Bayat, A-R., Kairenius, P., Viitala, S., Mutikainen, M., Leskinen, H., ... Vilkki, J. (2018). Dietary supplement of conjugated linoleic acid and polyunsaturated fatty acid suppressed the mobilization of body fat reserves from subcutaneous adipose tissue in dairy cows at early lactation through different pathways. P31. Poster session presented at Fifth DairyCare Conference, .
@conference{8db9c53f82ec4341b9f0438f15802283,
title = "Dietary supplement of conjugated linoleic acid and polyunsaturated fatty acid suppressed the mobilization of body fat reserves from subcutaneous adipose tissue in dairy cows at early lactation through different pathways",
abstract = "Milk fat depression (MFD) is an effective way to reduce the metabolic stress of dairy cows during lactation. Dietary supplement offatty acids can induce MFD by inhibiting mammary lipid synthesis. To investigate the impacts of MFD on the metabolism in theadipose tissue (AT), 30 cows were randomly assigned to a control diet, a conjugated linoleic acid supplemented diet (as CLA diet),or a high-starch diet supplemented with polyunsaturated fatty acids (sunflower oil: fish oil = 2:1; as HSO diet). Measurements ofanimal performance, milk yield, milk composition, energy balance, and blood metabolites were conducted at intervals duringlactation. The expression of candidate genes in AT was analyzed by quantitative PCR at wk 3 and 15 of lactation. Milk fat yield andcontent were effectively depressed by the CLA and HSO diets at wk 3 and wk 15 of lactation. Surprisingly, milk yield was alsoconsiderably decreased in the HSO group. The CLA and HSO diets improved energy balance at early lactation, supported by thereduced body weight loss and decreased plasma non-esterified fatty acid and β-hydroxybutyrate concentrations in these twogroups. Lipolysis in AT was suppressed by the CLA and HSO diets at early lactation through the downregulation of hormonesensitivelipase and fatty acid binding protein 4 and the upregulation of perilipin 2. Lipogenesis in AT was promoted only by theHSO diet at wk 15 through the upregulation of 1-acylglycerol-3-phosphate O-acyltransferase 2, mitochondrial glycerol-3-phosphateacyltransferase, perilipin 2, and peroxisome proliferator-activated receptor γ. In addition, the CLA diet led to increasingtranscription of various genes involved in insulin signaling, inflammatory responses, and ceramide metabolism, including proteinkinase B2, nuclear factor κ B1, toll-like receptor 4, caveolin 1, serine palmitoyltransferase long chain base subunit 1, and Nacylsphingosineamidohydrolase 1. The changes in these insulin-related pathways suggest likely altered insulin sensitivity in AT ofthe CLA group. In contrast, the HSO diet resulted in little or no change in these insulin-related pathways. In conclusion, the CLA andHSO diets shifted energy partitioning towards AT instead of mammary gland at early lactation through the regulation of differentpathways.",
keywords = "412 Animal science, dairy science",
author = "Nanbing Qin and Ali-Reza Bayat and Piia Kairenius and Sirja Viitala and Mervi Mutikainen and Heidi Leskinen and Elo, {Kari Tapani} and Kokkonen, {Tuomo Juhani} and Johanna Vilkki",
year = "2018",
month = "3",
language = "English",
pages = "P31",
note = "Fifth DairyCare Conference ; Conference date: 19-03-2018 Through 20-03-2018",

}

Dietary supplement of conjugated linoleic acid and polyunsaturated fatty acid suppressed the mobilization of body fat reserves from subcutaneous adipose tissue in dairy cows at early lactation through different pathways. / Qin, Nanbing; Bayat, Ali-Reza ; Kairenius, Piia; Viitala, Sirja; Mutikainen, Mervi; Leskinen, Heidi; Elo, Kari Tapani; Kokkonen, Tuomo Juhani; Vilkki, Johanna.

2018. P31 Poster session presented at Fifth DairyCare Conference, .

Research output: Conference materialsPosterResearchpeer-review

TY - CONF

T1 - Dietary supplement of conjugated linoleic acid and polyunsaturated fatty acid suppressed the mobilization of body fat reserves from subcutaneous adipose tissue in dairy cows at early lactation through different pathways

AU - Qin, Nanbing

AU - Bayat, Ali-Reza

AU - Kairenius, Piia

AU - Viitala, Sirja

AU - Mutikainen, Mervi

AU - Leskinen, Heidi

AU - Elo, Kari Tapani

AU - Kokkonen, Tuomo Juhani

AU - Vilkki, Johanna

PY - 2018/3

Y1 - 2018/3

N2 - Milk fat depression (MFD) is an effective way to reduce the metabolic stress of dairy cows during lactation. Dietary supplement offatty acids can induce MFD by inhibiting mammary lipid synthesis. To investigate the impacts of MFD on the metabolism in theadipose tissue (AT), 30 cows were randomly assigned to a control diet, a conjugated linoleic acid supplemented diet (as CLA diet),or a high-starch diet supplemented with polyunsaturated fatty acids (sunflower oil: fish oil = 2:1; as HSO diet). Measurements ofanimal performance, milk yield, milk composition, energy balance, and blood metabolites were conducted at intervals duringlactation. The expression of candidate genes in AT was analyzed by quantitative PCR at wk 3 and 15 of lactation. Milk fat yield andcontent were effectively depressed by the CLA and HSO diets at wk 3 and wk 15 of lactation. Surprisingly, milk yield was alsoconsiderably decreased in the HSO group. The CLA and HSO diets improved energy balance at early lactation, supported by thereduced body weight loss and decreased plasma non-esterified fatty acid and β-hydroxybutyrate concentrations in these twogroups. Lipolysis in AT was suppressed by the CLA and HSO diets at early lactation through the downregulation of hormonesensitivelipase and fatty acid binding protein 4 and the upregulation of perilipin 2. Lipogenesis in AT was promoted only by theHSO diet at wk 15 through the upregulation of 1-acylglycerol-3-phosphate O-acyltransferase 2, mitochondrial glycerol-3-phosphateacyltransferase, perilipin 2, and peroxisome proliferator-activated receptor γ. In addition, the CLA diet led to increasingtranscription of various genes involved in insulin signaling, inflammatory responses, and ceramide metabolism, including proteinkinase B2, nuclear factor κ B1, toll-like receptor 4, caveolin 1, serine palmitoyltransferase long chain base subunit 1, and Nacylsphingosineamidohydrolase 1. The changes in these insulin-related pathways suggest likely altered insulin sensitivity in AT ofthe CLA group. In contrast, the HSO diet resulted in little or no change in these insulin-related pathways. In conclusion, the CLA andHSO diets shifted energy partitioning towards AT instead of mammary gland at early lactation through the regulation of differentpathways.

AB - Milk fat depression (MFD) is an effective way to reduce the metabolic stress of dairy cows during lactation. Dietary supplement offatty acids can induce MFD by inhibiting mammary lipid synthesis. To investigate the impacts of MFD on the metabolism in theadipose tissue (AT), 30 cows were randomly assigned to a control diet, a conjugated linoleic acid supplemented diet (as CLA diet),or a high-starch diet supplemented with polyunsaturated fatty acids (sunflower oil: fish oil = 2:1; as HSO diet). Measurements ofanimal performance, milk yield, milk composition, energy balance, and blood metabolites were conducted at intervals duringlactation. The expression of candidate genes in AT was analyzed by quantitative PCR at wk 3 and 15 of lactation. Milk fat yield andcontent were effectively depressed by the CLA and HSO diets at wk 3 and wk 15 of lactation. Surprisingly, milk yield was alsoconsiderably decreased in the HSO group. The CLA and HSO diets improved energy balance at early lactation, supported by thereduced body weight loss and decreased plasma non-esterified fatty acid and β-hydroxybutyrate concentrations in these twogroups. Lipolysis in AT was suppressed by the CLA and HSO diets at early lactation through the downregulation of hormonesensitivelipase and fatty acid binding protein 4 and the upregulation of perilipin 2. Lipogenesis in AT was promoted only by theHSO diet at wk 15 through the upregulation of 1-acylglycerol-3-phosphate O-acyltransferase 2, mitochondrial glycerol-3-phosphateacyltransferase, perilipin 2, and peroxisome proliferator-activated receptor γ. In addition, the CLA diet led to increasingtranscription of various genes involved in insulin signaling, inflammatory responses, and ceramide metabolism, including proteinkinase B2, nuclear factor κ B1, toll-like receptor 4, caveolin 1, serine palmitoyltransferase long chain base subunit 1, and Nacylsphingosineamidohydrolase 1. The changes in these insulin-related pathways suggest likely altered insulin sensitivity in AT ofthe CLA group. In contrast, the HSO diet resulted in little or no change in these insulin-related pathways. In conclusion, the CLA andHSO diets shifted energy partitioning towards AT instead of mammary gland at early lactation through the regulation of differentpathways.

KW - 412 Animal science, dairy science

M3 - Poster

SP - P31

ER -