Dietary supplement of conjugated linoleic acids or polyunsaturated fatty acids suppressed the mobilization of body fat reserves in dairy cows at early lactation through different pathways

Nanbing Qin, Ali-Reza Bayat, Erminio Trevisi, Andrea Minuti, Piia Kairenius, Sirja Viitala, Mervi Mutikainen, Heidi Leskinen, Kari Tapani Elo, Tuomo Juhani Kokkonen, Johanna Vilkki

Research output: Contribution to journalArticleScientificpeer-review

Abstract

To investigate the metabolic (.11, !I:2.es in the adipose tissue (AT) of dairy cows under milk fat depression (MFD), 30 cows were randomly allocated to a control diet, a conjugated linoleic acid (CLA)-supplemented diet, or a high-starch diet supplemented with a mixture of sunflower and fish oil (2:1; as HSO diet) from 1 to 112 d in milk. Performance of animals, milk yield, milk composition, energy balance, and blood metabolites were measured during lactation. Quantitative PCR analyses were conducted on the AT samples collected at wk 3 and 15 of lactation. The CLA and HSO diets considerably depressed milk fat yield and milk fat content at both wk 3 and 15 in the absence of significant changes in milk protein and lactose contents. In addition, the HSO diet lowered milk yield at wk 15 and decreased dry matter intake of cows from wk 3 to 15. Compared with the control, both CLA and HSO groups showed reduced body weight loss, improved energy balance, and decreased plasma concentrations of nonesterified fatty acids and beta-hydroxybutyrate at early lactation. The gene expression analyses reflected suppressed lipolysis in AT of the CLA and HSO groups compared with the control at wk 3, as suggested by the downregulation of hormone-sensitive lipase and fatty acid binding protein 4 and the upregulation of perilipin 2. In addition, the HSO diet promoted lipogenesis in AT at wk 15 through the upregulation of 1-acylglycerol-3-phosphate O-acyltransferase 2, mitochondria' glycerol-3-phosphate acyltransferase, perilipin 2, and peroxisome proliferator-activated receptor gamma. The CLA diet likely regulated insulin sensitivity in AT as it upregulated the transcription of various genes involved in insulin signaling, inflammatory responses, and ceramide metabolism, including protein kinase B2, nuclear factor kappa B1, toll-like receptor 4, caveolin 1, serine palmitoyltransferase long chain base subunit 1, and N-acylsphingosine amidohydrolase 1. In contrast, the HSO diet resulted in little or no change in the pathways relevant to insulin sensitivity. In conclusion, the CLA and HSO diets induced a shift in energy partitioning toward AT instead of mammary gland during lactation through the regulation of different pathways.
Original languageEnglish
JournalJournal of Dairy Science
Volume101
Issue number9
Pages (from-to)7954-7970
Number of pages17
ISSN0022-0302
DOIs
Publication statusPublished - Sep 2018
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 412 Animal science, dairy science
  • milk fat depression
  • lipolysis
  • insulin resistance
  • gene expression
  • ceramide
  • SUBCUTANEOUS ADIPOSE-TISSUE
  • NF-KAPPA-B
  • MILK-FAT
  • TRANSITION PERIOD
  • FISH-OIL
  • INSULIN-RESISTANCE
  • LIPID-METABOLISM
  • GENE-EXPRESSION
  • LATE PREGNANCY
  • TRANSCRIPTIONAL ADAPTATIONS
  • milk fat depression
  • lipolysis
  • insulin resistance
  • gene expression
  • ceramide
  • SUBCUTANEOUS ADIPOSE-TISSUE
  • NF-KAPPA-B
  • MILK-FAT
  • TRANSITION PERIOD
  • FISH-OIL
  • INSULIN-RESISTANCE
  • LIPID-METABOLISM
  • GENE-EXPRESSION
  • LATE PREGNANCY
  • TRANSCRIPTIONAL ADAPTATIONS

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