Differentiation of Human Pluripotent Stem Cells Into Definitive Endoderm Cells in Various Flexible Three-Dimensional Cell Culture Systems: Possibilities and Limitations

Mariia S. Bogacheva, Riina Harjumäki, Emilia Flander, Ara Taalas, Margarita A. Bystriakova, Marjo Yliperttula, Xiaoqiang Xiang, Alan W. Leung, Yan Ru Lou

Research output: Contribution to journalArticleScientificpeer-review

Abstract

The generation of human stem cell-derived spheroids and organoids represents a major step in solving numerous medical, pharmacological, and biological challenges. Due to the advantages of three-dimensional (3D) cell culture systems and the diverse applications of human pluripotent stem cell (iPSC)-derived definitive endoderm (DE), we studied the influence of spheroid size and 3D cell culture systems on spheroid morphology and the effectiveness of DE differentiation as assessed by quantitative PCR (qPCR), flow cytometry, immunofluorescence, and computational modeling. Among the tested hydrogel-based 3D systems, we found that basement membrane extract (BME) hydrogel could not retain spheroid morphology due to dominant cell–matrix interactions. On the other hand, we found that nanofibrillar cellulose (NFC) hydrogel could maintain spheroid morphology but impeded growth factor diffusion, thereby negatively affecting cell differentiation. In contrast, suspension culture provided sufficient mass transfer and was demonstrated by protein expression assays, morphological analyses, and mathematical modeling to be superior to the hydrogel-based systems. In addition, we found that spheroid size was reversely correlated with the effectiveness of DE formation. However, spheroids of insufficient sizes failed to retain 3D morphology during differentiation in all the studied culture conditions. We hereby demonstrate how the properties of a chosen biomaterial influence the differentiation process and the importance of spheroid size control for successful human iPSC differentiation. Our study provides critical parametric information for the generation of human DE-derived, tissue-specific organoids in future studies.

Original languageEnglish
Article number726499
JournalFrontiers in Cell and Developmental Biology
Volume9
Number of pages20
ISSN2296-634X
DOIs
Publication statusPublished - 9 Sep 2021
MoE publication typeA1 Journal article-refereed

Fields of Science

  • 317 Pharmacy
  • 1182 Biochemistry, cell and molecular biology
  • 318 Medical biotechnology

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