Discovering protein kinase C active plants growing in Finland utilizing automated bioassay combined to LC/MS

Research output: Contribution to journalArticleScientificpeer-review

Abstract

Protein kinase C (PKC) modulating activity of 81 plant extracts of Finnish origin was investigated with an automated bioassay method combined to LC/MS. Twenty-one extracts from different parts of the plants inhibited PKC significantly. Fractionation of the active extract of Filipendula ulmaria showed that this method was able to identify a PKC inhibiting compound from the extract as quercetin. Our results indicate that this method is suitable for PKC screening of complex matrices and provides a quick and low volume, non-radioactive, alternative method for PKC experiments.
Original languageEnglish
JournalNatural Product Communications
Volume4
Issue number1
Pages (from-to)139-142
Number of pages4
ISSN1934-578X
Publication statusPublished - 2009
MoE publication typeA1 Journal article-refereed

Cite this

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title = "Discovering protein kinase C active plants growing in Finland utilizing automated bioassay combined to LC/MS",
abstract = "Protein kinase C (PKC) modulating activity of 81 plant extracts of Finnish origin was investigated with an automated bioassay method combined to LC/MS. Twenty-one extracts from different parts of the plants inhibited PKC significantly. Fractionation of the active extract of Filipendula ulmaria showed that this method was able to identify a PKC inhibiting compound from the extract as quercetin. Our results indicate that this method is suitable for PKC screening of complex matrices and provides a quick and low volume, non-radioactive, alternative method for PKC experiments.",
author = "Anna Galkin and Jouni Jokela and Matti Wahlsten and P{\"a}ivi Tammela and Kaarina Sivonen and Pia Vuorela",
year = "2009",
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volume = "4",
pages = "139--142",
journal = "Natural Product Communications",
issn = "1934-578X",
publisher = "Natural Product Communications",
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Discovering protein kinase C active plants growing in Finland utilizing automated bioassay combined to LC/MS. / Galkin, Anna; Jokela, Jouni; Wahlsten, Matti; Tammela, Päivi; Sivonen, Kaarina; Vuorela, Pia.

In: Natural Product Communications, Vol. 4, No. 1, 2009, p. 139-142.

Research output: Contribution to journalArticleScientificpeer-review

TY - JOUR

T1 - Discovering protein kinase C active plants growing in Finland utilizing automated bioassay combined to LC/MS

AU - Galkin, Anna

AU - Jokela, Jouni

AU - Wahlsten, Matti

AU - Tammela, Päivi

AU - Sivonen, Kaarina

AU - Vuorela, Pia

PY - 2009

Y1 - 2009

N2 - Protein kinase C (PKC) modulating activity of 81 plant extracts of Finnish origin was investigated with an automated bioassay method combined to LC/MS. Twenty-one extracts from different parts of the plants inhibited PKC significantly. Fractionation of the active extract of Filipendula ulmaria showed that this method was able to identify a PKC inhibiting compound from the extract as quercetin. Our results indicate that this method is suitable for PKC screening of complex matrices and provides a quick and low volume, non-radioactive, alternative method for PKC experiments.

AB - Protein kinase C (PKC) modulating activity of 81 plant extracts of Finnish origin was investigated with an automated bioassay method combined to LC/MS. Twenty-one extracts from different parts of the plants inhibited PKC significantly. Fractionation of the active extract of Filipendula ulmaria showed that this method was able to identify a PKC inhibiting compound from the extract as quercetin. Our results indicate that this method is suitable for PKC screening of complex matrices and provides a quick and low volume, non-radioactive, alternative method for PKC experiments.

M3 - Article

VL - 4

SP - 139

EP - 142

JO - Natural Product Communications

JF - Natural Product Communications

SN - 1934-578X

IS - 1

ER -